Identification and characterization of a novel cathelicidin from ayu, Plecoglossus altivelis

Lu, Xin-Jiang; Chen, J.; Huang, Zuo-An; Shi, Yu; Lu, Jianying

doi:10.1016/j.fsi.2011.03.005

Cited by 50 publications

(29 citation statements)

References 25 publications

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“…Overnight cultures of L. anguillarum were diluted 1:50 in nutrient broth, grown at 28 C with shaking and harvested in the log phase. Cells were resuspended in sterile phosphatebuffered saline (PBS) and adjusted to a final concentration of 1.0 Â 10 6 CFU/mL [15,17]. Forty fish were intraperitoneally injected with 100 mL of L. anguillarum per fish [15]; the 20 remaining fish were injected with 100 mL of PBS per fish as a negative control.…”

Section: A1-antitrypsinmentioning

confidence: 99%

“…The partial sequence of ayu AAT cDNA was obtained from a previously established cDNA library [17]. Based on this, the fulllength AAT cDNA sequence was determined by using the Rapid Amplification of cDNA Ends (RACE) method [18].…”

Section: A1-antitrypsinmentioning

confidence: 99%

“…RNA extraction, firststrand cDNA synthesis and real-time quantitative PCR were carried out as described previously [17]. Four biological replicates each and three technical replicates for each individual were performed.…”

Section: A1-antitrypsinmentioning

confidence: 99%

“…Primers aAATF: 5 0 -AGTTCGCCTTCCGCCTCTAC-3 0 and aAATR: 5 0 -GTTTGGCCTCTTGCACC TAC-3 0 were designed to amplify a 127-base pair (bp) fragment from ayu AAT cDNA. As an internal PCR control, primers pActin2(þ): 5 0 -TCGTGCGTGACATCAAGGAG-3 0 and pActin2(À): 5 0 -CGCACTTCATGATGCTGTTG-3 0 were used to amplify a 231-bp fragment of the housekeeping b-actin gene [17]. The ayu AAT mRNA expression was normalised against b-actin expression.…”

Section: A1-antitrypsinmentioning

confidence: 99%

See 3 more Smart Citations

Identification of α1-antitrypsin as a positive acute phase protein in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Lu¹,

Chen²,

Lü³

et al. 2012

Fish & Shellfish Immunology

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Section: A1-antitrypsinmentioning

confidence: 99%

Section: A1-antitrypsinmentioning

confidence: 99%

Section: A1-antitrypsinmentioning

confidence: 99%

Section: A1-antitrypsinmentioning

confidence: 99%

See 2 more Smart Citations

Identification of α1-antitrypsin as a positive acute phase protein in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Lu¹,

Chen²,

Lü³

et al. 2012

Fish & Shellfish Immunology

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“…Four individuals per treatment were used for gene expression analysis. RNA extraction, first-strand cDNA synthesis and RT-qPCR were carried out as described previously [12]. All data were expressed as means AE SEM, and analyzed by one-way analysis of variance (ANOVA) with SPSS version 13.0 (SPSS Inc, Chicago, USA).…”

Section: Introductionmentioning

confidence: 99%

Use of suppressive subtractive hybridization to identify differentially expressed genes in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Chen

Shi

et al. 2011

Fish & Shellfish Immunology

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Comparison of vertebrate skin structure at class level: A review

Akat

Yenmiş

Pombal

et al. 2022

The Anatomical Record

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The skin is a barrier between the internal and external environment of an organism. Depending on the species, it participates in multiple functions. The skin is the organ that holds the body together, covers and protects it, and provides communication with its environment. It is also the body's primary line of defense, especially for anamniotes. All vertebrates have multilayered skin composed of three main layers: the epidermis, the dermis, and the hypodermis. The vital mission of the integument in aquatic vertebrates is mucus secretion. Cornification began in apmhibians, improved in reptilians, and endured in avian and mammalian epidermis. The feather, the most ostentatious and functional structure of avian skin, evolved in the Mesozoic period. After the extinction of the dinosaurs, birds continued to diversify, followed by the enlargement, expansion, and diversification of mammals, which brings us to the most complicated skin organization of mammals with differing glands, cells, physiological pathways, and the evolution of hair. Throughout these radical changes, some features were preserved among classes such as basic dermal structure, pigment cell types, basic coloration genetics, and similar sensory features, which enable us to track the evolutionary path. The structural and physiological properties of the skin in all classes of vertebrates are presented. The purpose of this review is to go all the way back to the agnathans and follow the path step by step up to mammals to provide a comparative large and updated survey about vertebrate skin in terms of morphology, physiology, genetics, ecology, and immunology.

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Identification and characterization of a novel cathelicidin from ayu, Plecoglossus altivelis

Cited by 50 publications

References 25 publications

Identification of α1-antitrypsin as a positive acute phase protein in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Identification of α1-antitrypsin as a positive acute phase protein in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Use of suppressive subtractive hybridization to identify differentially expressed genes in ayu (Plecoglossus altivelis) associated with Listonella anguillarum infection

Comparison of vertebrate skin structure at class level: A review

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