Identification and characterization of a mannose-binding lectin from Nile tilapia (Oreochromis niloticus)

Mu, Liangliang; Yin, Xiaoxue; Liu, Jing; Wu, Liting; Bian, Xia; Wang, Yuhong; Ye, Jianmin

doi:10.1016/j.fsi.2017.06.016

Cited by 93 publications

(46 citation statements)

References 37 publications

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“…The spleen leucocytes were segregated from Nile Tilapia by Histopaque ® 1077 (Sigma, USA) as previous reports (Mu et al, ; Wu et al, ). Simply, spleen was grinded in a flask, stacked on top of 1077 and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…The leucocytes were counted and re‐suspended with/L15 medium, containing 1% penicillin/streptomycin (Sigma, USA) and 10% foetal bovine serum (FBS) (Gibco, USA). The cells were cultured in 96‐well plate (Corning, USA; 100 μl/well) with the final concentration of 1 × 10 6 cells/ml per well and then stimulated with poly (I:C) (50 μg/ml), S. agalactiae (1 × 10 7 CFU/ml) (Mu et al, ; Wang, Seymour, Sundberg, & Hogenesch, ) and A. hydrophila (1 × 10 7 CFU/ml) at 25℃. Bacteria of S. agalactiae and A. hydrophila (Tang et al, ; Yin et al, ) were cultured and inactivated and control group with the equal volume of 1 × PBS as same as our previous study.…”

Section: Methodsmentioning

confidence: 99%

“…The spleen leucocytes were segregated from Nile Tilapia by Histopaque ® 1077 (Sigma, USA) as previous reports (Mu et al, 2017;Wu et al, 2019).…”

Section: Isolation and Stimulation Of Leucocytes In Vitromentioning

confidence: 99%

“…agalactiae (1 × 10 7 CFU/ml) (Mu et al, 2017; and A. hydrophila (1 × 10 7 CFU/ml) at 25℃. Bacteria of S. agalactiae and A. hydrophila (Tang et al, 2014;Yin et al, 2018) were cultured and inactivated and control group with the equal volume of 1 × PBS as same as our previous study.…”

Section: Primersmentioning

confidence: 99%

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CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

Kong

et al. 2020

Aquac Res

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CD3 is an important membrane molecule of mature T cells. TCR‐CD3 complex plays important roles in the activation and signal transduction of T cells. In this study, the CD3ε (OnCD3ε) gene was cloned from Nile tilapia (Oreochromis niloticus), and its tissue distribution was detected by qPCR. The expression changes in CD3ε were analysed after three challenges (S. agalactiae, A. hydrophila and poly (I:C)) in vivo and in vitro. The open reading frame (ORF) of OnCD3ε contains 531 bp, encoding 176 amino acids, which found that OnCD3ε consists of conserved amino acid residues and motifs (cysteine residues, CXXC motif, immunoreceptor tyrosine‐based activation motifs and PxPDY). In addition, tissue distribution displayed that OnCD3ε has the highest expression in thymus and significant expression level in mucosal immune‐related tissues (intestine and gills). In vivo, all three challenges could significantly up‐regulate the expression of CD3ε in the head kidney and spleen. For further in vitro assays, after poly (I:C) stimulation, OnCD3ε appeared significant up‐regulation at 3 hr post stimulation, and two pathogenic bacteria also induce significant up‐regulation of OnCD3ε in spleen leucocytes (12 hr). These results suggested that OnCD3ε was likely to get involved in host immune response against pathogenic challenges.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The spleen leucocytes were segregated from Nile Tilapia by Histopaque ® 1077 (Sigma, USA) as previous reports (Mu et al, 2017;Wu et al, 2019).…”

Section: Isolation and Stimulation Of Leucocytes In Vitromentioning

confidence: 99%

Section: Primersmentioning

confidence: 99%

See 2 more Smart Citations

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

Kong

et al. 2020

Aquac Res

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“…Protein conformation is influenced by the ions present, temperature, and pH that specifically influences the agglutination capacity of the proteins. Therefore, heating can inhibit agglutination [ 27 ] and agglutination can be Ca ++ dependent [ 27 , 28 ]. Lectins that only have one carbohydrate recognising domain (CRD) must form dimers or higher-order structures to be able to agglutinate.…”

Section: The Role Of Mucosal Lectins In Agglutination and Hemagglumentioning

confidence: 99%

Functional Aspects of Fish Mucosal Lectins—Interaction with Non-Self

et al. 2018

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Mucosal surfaces are of key importance in protecting animals against external threats including pathogens. In the mucosal surfaces, host molecules interact with non-self to prevent infection and disease. Interestingly, both inhibition and stimulation of uptake hinder infection. In this review, the current knowledgebase on teleost mucosal lectins’ ability to interact with non-self is summarised with a focus on agglutination, growth inhibition, opsonisation, cell adhesion, and direct killing activities. Further research on lectins is essential, both to understand the immune system of fishes, since they rely more on the innate immune system than mammals, and also to explore these molecules’ antibiotic and antiparasitic activities against veterinary and human pathogens.

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Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

Lapitan,

Felisilda,

Tiangco

et al. 2024

Chemistry An Asian Journal

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Pseudomonas aeruginosa is an opportunistic pathogen that infects wounds and burns and causes severe infections in humans. The high virulence, the emergence of antibiotic‐resistant strains, and the easy transmissibility of P. aeruginosa necessitate its fast detection and control. The gold standard for detecting P. aeruginosa, the plate culture method, though reliable, takes several days to complete. Therefore, developing accurate, rapid, and easy‐to‐use diagnostic tools for P. aeruginosa is highly desirable. Nanomaterial‐based biosensors are at the forefront of detecting P. aeruginosa and its secondary metabolites. This review summarises the biorecognition elements, biomarkers, immobilisation strategies, and current state‐of‐the‐art biosensors for P. aeruginosa. The review highlights the underlying principles of bioreceptor layer engineering and the design of nanomaterial‐based optical, electrochemical, mass‐based, and thermal biosensors. The advantages and disadvantages of these biosensors and their future point‐of‐care applications are also discussed.

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Identification and characterization of a mannose-binding lectin from Nile tilapia (Oreochromis niloticus)

Cited by 93 publications

References 37 publications

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

CD3ε is involved in host immune response against challenges in Nile tilapia (Orechromis niloticus)

Functional Aspects of Fish Mucosal Lectins—Interaction with Non-Self

Advances in Bioreceptor Layer Engineering in Nanomaterial‐based Sensing of Pseudomonas Aeruginosa and its Metabolites

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