“…Moreover, previous studies have reported that phlorotannin also inhibits the activity of several other enzymes, such as hyaluronidase (Shibata et al, 2002), -glucosidase (Kellogg et al, 2014) and -amylase (Kellogg et al, 2014). Interestingly, Eisenia hydrolysis-enhancing protein (EHEP), which was recently identified in the digestive fluid of A. kurodai, protects akuBGLs from this inhibition by binding and then precipitating with phlorotannin, despite not possessing a catalytic (Tsuji et al, 2017). Furthermore, it has been shown that EHEP is a novel and unique protein, with no proteins with homologous amino-acid sequences occurring in any other organism, and it also binds to analogs of phlorotannin such as gallic acid and tannic acid (Tsuji et al, 2017).…”