Identification and characterization of multiple abiotic stress tolerance genes in wheat

Kumar, Rakesh; Masthigowda, Mamrutha Harohalli; Kaur, Amandeep; Bhusal, Nabin; Pandey, Ankita; Kumar, Satish; Mishra, C. N.; Singh, Gyanendra Pratap

doi:10.1007/s11033-020-05906-5

Cited by 16 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Wheat is an important source of carbohydrates, protein, vitamins, and minerals for humans [ 61 , 62 , 63 , 64 ]. However, wheat production is adversely affected by several biotic and abiotic stresses such as insects, fungal, bacterial, and viral diseases, heat, drought, cold, and salinity [ 63 , 65 , 66 ]. Therefore, several researchers have concentrated on improving productivity, quality, and stress tolerance in wheat.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L.

Kumar

Kherawat

Dey

et al. 2021

IJMS

View full text Add to dashboard Cite

PIN-FORMED (PIN) genes play a crucial role in regulating polar auxin distribution in diverse developmental processes, including tropic responses, embryogenesis, tissue differentiation, and organogenesis. However, the role of PIN-mediated auxin transport in various plant species is poorly understood. Currently, no information is available about this gene family in wheat (Triticum aestivum L.). In the present investigation, we identified the PIN gene family in wheat to understand the evolution of PIN-mediated auxin transport and its role in various developmental processes and under different biotic and abiotic stress conditions. In this study, we performed genome-wide analysis of the PIN gene family in common wheat and identified 44 TaPIN genes through a homology search, further characterizing them to understand their structure, function, and distribution across various tissues. Phylogenetic analyses led to the classification of TaPIN genes into seven different groups, providing evidence of an evolutionary relationship with Arabidopsis thaliana and Oryza sativa. A gene exon/intron structure analysis showed a distinct evolutionary path and predicted the possible gene duplication events. Further, the physical and biochemical properties, conserved motifs, chromosomal, subcellular localization, transmembrane domains, and three-dimensional (3D) structure were also examined using various computational approaches. Cis-elements analysis of TaPIN genes showed that TaPIN promoters consist of phytohormone, plant growth and development, and stress-related cis-elements. In addition, expression profile analysis also revealed that the expression patterns of the TaPIN genes were different in different tissues and developmental stages. Several members of the TaPIN family were induced during biotic and abiotic stress. Moreover, the expression patterns of TaPIN genes were verified by qRT-PCR. The qRT-PCR results also show a similar expression with slight variation. Therefore, the outcome of this study provides basic genomic information on the expression of the TaPIN gene family and will pave the way for dissecting the precise role of TaPINs in plant developmental processes and different stress conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L.

Kumar

Kherawat

Dey

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Wheat is an essential source of carbohydrates, protein, vitamins, and minerals for humans [51][52][53][54]. However, wheat production is adversely affected by several biotic and abiotic stresses such as insects, fungal, bacterial, viral diseases, heat, drought, cold, and salinity [55,56]. Several researchers have been concentrated on improving productivity, quality and stress tolerance in wheat.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the Brassinazole-resistant (BZR) Gene Family and Its Expression in the Various Developmental Stage and Stress Conditions in Wheat (Triticum aestivum L.)

Kesawat

Kherawat

Singh

et al. 2021

IJMS

View full text Add to dashboard Cite

Brassinosteroids (BRs) play crucial roles in various biological processes, including plant developmental processes and response to diverse biotic and abiotic stresses. However, no information is currently available about this gene family in wheat (Triticum aestivum L.). In the present investigation, we identified the BZR gene family in wheat to understand the evolution and their role in diverse developmental processes and under different stress conditions. In this study, we performed the genome-wide analysis of the BZR gene family in the bread wheat and identified 20 TaBZR genes through a homology search and further characterized them to understand their structure, function, and distribution across various tissues. Phylogenetic analyses lead to the classification of TaBZR genes into five different groups or subfamilies, providing evidence of evolutionary relationship with Arabidopsis thaliana, Zea mays, Glycine max, and Oryza sativa. A gene exon/intron structure analysis showed a distinct evolutionary path and predicted the possible gene duplication events. Further, the physical and biochemical properties, conserved motifs, chromosomal, subcellular localization, and cis-acting regulatory elements were also examined using various computational approaches. In addition, an analysis of public RNA-seq data also shows that TaBZR genes may be involved in diverse developmental processes and stress tolerance mechanisms. Moreover, qRT-PCR results also showed similar expression with slight variation. Collectively, these results suggest that TaBZR genes might play an important role in plant developmental processes and various stress conditions. Therefore, this work provides valuable information for further elucidate the precise role of BZR family members in wheat.

show abstract

“…Heat treatments (H, HD, SH, SHD) had upregulation of many genes associated with abiotic stress, heat shock factors and proteolysis (Figure S1). These responses have been widely documented for salt and heat stress (Abhinandan et al., 2018; Farooq et al., 2017; Kumar et al., 2020; Zandalinas et al., 2020). Interestingly, drought treatments (D, HD, SD, SHD) only shared six DEGs.…”

Section: Resultsmentioning

confidence: 91%

Multi‐omics atlas of combinatorial abiotic stress responses in wheat

Da Ros,

Bollina,

Soolanayakanahally

et al. 2023

The Plant Journal

View full text Add to dashboard Cite

SUMMARYField‐grown crops rarely experience growth conditions in which yield can be maximized. Environmental stresses occur in combination, with advancements in crop tolerance further complicated by its polygenic nature. Strategic targeting of causal genes is required to meet future crop production needs. Here, we employed a systems biology approach in wheat (Triticum aestivum L.) to investigate physio‐metabolic adjustments and transcriptome reprogramming involved in acclimations to heat, drought, salinity and all combinations therein. A significant shift in magnitude and complexity of plant response was evident across stress scenarios based on the agronomic losses, increased proline concentrations and 8.7‐fold increase in unique differentially expressed transcripts (DETs) observed under the triple stress condition. Transcriptome data from all stress treatments were assembled into an online, open access eFP browser for visualizing gene expression during abiotic stress. Weighted gene co‐expression network analysis revealed 152 hub genes of which 32% contained the ethylene‐responsive element binding factor‐associated amphiphilic repression (EAR) transcriptional repression motif. Cross‐referencing against the 31 DETs common to all stress treatments isolated TaWRKY33 as a leading candidate for greater plant tolerance to combinatorial stresses. Integration of our findings with available literature on gene functional characterization allowed us to further suggest flexible gene combinations for future adaptive gene stacking in wheat. Our approach demonstrates the strength of robust multi‐omics‐based data resources for gene discovery in complex environmental conditions. Accessibility of such datasets will promote cross‐validation of candidate genes across studies and aid in accelerating causal gene validation for crop resiliency.

show abstract

Identification and characterization of multiple abiotic stress tolerance genes in wheat

Cited by 16 publications

References 40 publications

Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L.

Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L.

Genome-Wide Identification and Characterization of the Brassinazole-resistant (BZR) Gene Family and Its Expression in the Various Developmental Stage and Stress Conditions in Wheat (Triticum aestivum L.)

Multi‐omics atlas of combinatorial abiotic stress responses in wheat

Contact Info

Product

Resources

About