Identification and characterization of putative CIPK genes in maize

Chen, Xifeng; Gu, Zhenxin; Xin, Dedong; Hao, Linlin; Liu, Chengjie; Huang, Ji; Ma, Bo; Zhang, Hongsheng

doi:10.1016/j.jcg.2011.01.005

Cited by 104 publications

(98 citation statements)

References 48 publications

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“…Zea mays CIPK2 is upregulated by salt, PEG, cold, and heat; however, its expression differs between the leaves and the root (Chen et al, 2011). In the halophyte Hordeum brevisubulatum, HbCIPK2 is strongly induced in the root and shoot by high salinity, drought, and ABA treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequent studies have demonstrated the functional conservation of the CBL-CIPK pathway in Populus (Yu et al, 2007), grape (Weinl and Kudla, 2009), sorghum (Li et al, 2010), and maize (Chen et al, 2011). Enhanced tolerance to diverse stresses has also been regularly observed in transgenic Arabidopsis because of the overexpression of CIPKs (Zhao et al, 2009;Chen et al, 2012), rice (Xiang et al, 2007), tomato Huertas et al, 2012), tobacco (Tripathi et al, 2009), and apple (Hu et al, 2012).…”

Section: Introductionmentioning

confidence: 97%

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Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

et al. 2014

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ABSTRACT. CBL-interacting protein kinases (CIPKs) mediate many plant responses to abiotic stress. However, their functions are poorly understood in halophytes. In this study, we isolated a CIPK gene, NtCIPK2, from the halophyte Nitraria tangutorum. By sequence alignment and the construction of a phylogenetic tree, we found that NtCIPK2 is similar to CIPK2 proteins from other plants, and contains conserved domains and motifs. The promoter of NtCIPK2 harbors many cis-acting elements that might be recognized and bound by transcription factors that are related to hormones and stress responses. NtCIPK2 was ubiquitously and robustly expressed in all tested organs, and was induced by salinity, drought, heat, and cold stress. The overexpression of NtCIPK2 in Escherichia coli caused better growth against high salinity, alkalinity, and osmotic conditions, dehydration, and extreme temperatures (i.e., heat and cold) compared to the control. 4717©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 4716-4728 (2014) Cloning and characterization of the NtCIPK2 gene Thus, NtCIPK2 is a candidate gene that might improve the stress tolerance of crops and herbs through genetic manipulation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

et al. 2014

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“…Taking into account the functional divergence of the ATG6 family, ATG6 proteins are believed to be differently regulated under different stresses. Since the microarray data used in the analysis were obtained for a single time point and most of the changes induced by long-term stress stimuli cannot be detected by short time-course microarray (Chen et al, 2011), we further analyzed the expression of rice ATG6 genes under exposure to abiotic stresses for different time periods. We found that OsATG6 genes were expressed differentially under different stresses and that the expression pattern differed among the genes, suggesting a distinct stress-specific response.…”

Section: Discussionmentioning

confidence: 99%

Regulation of ATG6/Beclin-1 homologs by abiotic stresses and hormones in rice (Oryza sativa L.)

Rana¹,

Dong²,

Ali³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Autophagy, a complex and conserved mechanism, serving as a defense response in all eukaryotic organisms, is regulated by several proteins, among which ATG proteins are the most important due to their involvement in autophagosome formation. ATG6/Beclin-1 proteins, reported to be essential for autophagosome formation and assigned as a conserved domain, were subjected to database searches. We found three homologs in the rice (Oryza sativa) genome. A phylogeny tree was constructed to establish their across species relationship, which divided them into three distinct groups; two for plants, i.e., monocots and dicots, and one for animals. Evolutionary study of this family by critical amino acid conservation analysis revealed significant functional divergence. The finding of important stress-related cis-acting elements in the promoter region of rice ATG6 genes demonstrated their involvement in 3677 Regulation of ATG6 homologs by abiotic stresses and hormones ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 3676-3687 (2012) abiotic stress responses. Furthermore, expression profiling of rice ATG6 genes based on microarray data, as well as by semi-quantitative RT-PCR, showed differential expression when subjected to different stresses suggesting the involvement of OsATG6 genes in abiotic stresses (heat, cold and drought) and hormone (abscisic acid) responses. Analysis of co-expressed genes showed that most of them annotated to DNA repair pathways and proteolysis, etc. Collectively, these results suggest the involvement of OsATG6 genes in different stresses, and provide a basis for further functional studies to investigate the biological mechanism of action of these genes under abiotic stresses.

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“…The plant's transcriptional changes during drought stress have been extensively studied in a wide range of species, including Arabidopsis (Seki et al, , 2002Kilian et al, 2007;Huang et al, 2008;Matsui et al, 2008), oilseed rape (Brassica napus) , rice (Oryza sativa) (Lenka et al, 2011;, maize (Luo et al, 2010;Chen et al, 2011), loblolly pine (Pinus taeda) (Lorenz et al, 2011), and banana (Musa spp) (Davey et al, 2009). Analyses of gene expression, transcriptional regulation, and signal transduction in plants subjected to drought treatments have revealed pathways involved in plant response to water stress Abe et al, 2003;Tran et al, 2004).…”

Section: Classical Approaches For Tackling Drought Stressmentioning

confidence: 99%

“…Large numbers of signaling genes are regulated by drought stress in, for example, maize (Luo et al, 2010;Chen et al, 2011), but approaches to modify their activities in maize and other crops remain scarce. Furthermore, most of the genetically modified lines that have been developed to better withstand drought and other stress conditions have yet to be tested in the field (Tognetti et al, 2006;Nelson et al, 2007;Vanderauwera et al, 2007;Castiglioni et al, 2008).…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Tackling Drought Stress: RECEPTOR-LIKE KINASES Present New Approaches

et al. 2012

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Global climate change and a growing population require tackling the reduction in arable land and improving biomass production and seed yield per area under varying conditions. One of these conditions is suboptimal water availability. Here, we review some of the classical approaches to dealing with plant response to drought stress and we evaluate how research on RECEPTOR-LIKE KINASES (RLKs) can contribute to improving plant performance under drought stress. RLKs are considered as key regulators of plant architecture and growth behavior, but they also function in defense and stress responses. The available literature and analyses of available transcript profiling data indeed suggest that RLKs can play an important role in optimizing plant responses to drought stress. In addition, RLK pathways are ideal targets for nontransgenic approaches, such as synthetic molecules, providing a novel strategy to manipulate their activity and supporting translational studies from model species, such as Arabidopsis thaliana, to economically useful crops.

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Identification and characterization of putative CIPK genes in maize

Cited by 104 publications

References 48 publications

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

Molecular cloning and functional characterization of a novel CBL-interacting protein kinase NtCIPK2 in the halophyte Nitraria tangutorum

Regulation of ATG6/Beclin-1 homologs by abiotic stresses and hormones in rice (Oryza sativa L.)

Tackling Drought Stress: RECEPTOR-LIKE KINASES Present New Approaches

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