Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions

Liu, Chenlin; Xiao, Zhao; Wang, Xiuliang

doi:10.1007/s10811-018-1492-4

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…IBPs originating from HGT were also discussed in the transcriptomic and genomic study of C. ICE-L ( Liu C. et al, 2016 ; Zhang et al, 2020 ), and in the fungus Antarctica psychrotrophicus ( Arai et al, 2019 ). In addition to IBPs, heat-shock proteins in C. ICE-L, and ATPases in the red alga Galdieria sulphuraria were identified as horizontally transferred genes that also play a role in cold acclimation ( Liu et al, 2018 ; Rossoni et al, 2019 ). SAM dependent carboxyl methyltransferases catalyze the transfer of a methyl group from SAM to a variety of substrates, indicating specific methylated derivatives in C. nivalis .…”

Section: Discussionmentioning

confidence: 99%

Cold Adaptation Mechanisms of a Snow Alga Chlamydomonas nivalis During Temperature Fluctuations

2021

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Cold environments, such as glaciers and alpine regions, constitute unique habitats for organisms living on Earth. In these harsh ecosystems, snow algae survive, florish, and even become primary producers for microbial communities. How the snow algae maintain physiological activity during violent ambient temperature changes remains unsolved. To explore the cold adaptation mechanisms of the unicellular snow alga Chlamydomonas nivalis, we compared its physiological responses to a model organism from the same genus, Chlamydomonas reinhardtii. When both cell types were exposed to a shift from 22°C to 4°C, C. nivalis exhibited an apparent advantage in cold tolerance over C. reinhardtii, as C. nivalis had both a higher growth rate and photosynthetic efficiency. To determine the cold tolerance mechanisms of C. nivalis, RNA sequencing was used to compare transcriptomes of both species after 1 h of cold treatment, mimicking temperature fluctuations in the polar region. Differential expression analysis showed that C. nivalis had fewer transcriptomic changes and was more stable during rapid temperature decrease relative to C. reinhardtii, especially for the expression of photosynthesis related genes. Additionally, we found that transcription in C. nivalis was precisely regulated by the cold response network, consisting of at least 12 transcription factors and 3 RNA-binding proteins. Moreover, genes participating in nitrogen metabolism, the pentose phosphate pathway, and polysaccharide biosynthesis were upregulated, indicating that increasing resource assimilation and remodeling of metabolisms were critical for cold adaptation in C. nivalis. Furthermore, we identified horizontally transferred genes differentially expressed in C. nivalis, which are critical for cold adaptation in other psychrophiles. Our results reveal that C. nivalis adapts rapid temperature decrease by efficiently regulating transcription of specific genes to optimize resource assimilation and metabolic pathways, providing critical insights into how snow algae survive and propagate in cold environments.

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Section: Discussionmentioning

confidence: 99%

Cold Adaptation Mechanisms of a Snow Alga Chlamydomonas nivalis During Temperature Fluctuations

2021

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“…The differences we report in strains’ tolerance to freezing may be related to differences in their capacities to form plasmalemma structures associated with freeze tolerance [ 34 ], or to differences in stress–response proteins, such asAsp-Glu-Ala-Asp (DEAD)-box RNA helicase proteins [ 35 ] and Hsp70, variants of which appear to underlie the psychrophilic habit of Antarctic sea-ice Chlamydomonas sp. ICE-L [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of clonal and polyclonal populations of Chlamydomonas reinhardtii

Boswell

Lindsey

Cook

et al. 2021

Biology Methods and Protocols

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Long-term preservation of laboratory strains of Chlamydomonas reinhardtii has historically involved either liquid nitrogen cryopreservation, which is expensive and labor intensive, or storage on agar plates, which requires frequent transfer to new plates, and which may leave samples susceptible to contamination as well as genetic drift and/or selection. The emergence of Chlamydomonas reinhardtii as a model organism for genetic analysis and experimental evolution has produced an increasing demand for an efficient method to cryopreserve C. reinhardtii populations. The GeneArt™ Cryopreservation Kit for Algae provides the first method for algal storage at - 80 °C; however, little is known about how this method affects recovery of different clones, much less polyclonal populations. Here, we compare post-freeze viability of clonal and genetically mixed samples frozen at -80 °C using GeneArt™ or cryopreserved using liquid nitrogen. We find that the GeneArt™ protocol yields similar percent recoveries for some but not all clonal cultures, when compared to archiving via liquid N2. We also find that relative frequency of different strains recovered from genetically mixed populations can be significantly altered by cryopreservation. Thus, while cryopreservation using GeneArt™ is an effective means for archiving certain clonal populations it is not universally so. Strain-specific differences in freeze-thaw tolerance complicate the storage of different clones, and may also bias the recovery of different genotypes from polyclonal populations.

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“…PR assists in cellular growth and survival during high environmental stress, such as salinity stresses similar to those in sea-ice brine channels [31]. YR1-1 T also contained proteins for the molecular chaperone DnaK (COG0484 and COG0576, UniProtKB/ PIR accession number: A0A6B3QYM3), this protein can prevent irreversible protein denaturation by chaperoning the unfolded polypeptides under extremely cold environments, and enhance cold and freezing tolerance of microorganisms [32]. The genome of YR1-1 T encoded a protein (COG1970, UniProtKB/PIR accession number: A0A6B3QYD9) that may participate in the regulation of osmotic pressure changes within the cell by opening channels in response to stretch forces.…”

Section: Psychroflexus Aurantiacus Yr1-1 T (Mn629180) Psychroflexus Smentioning

confidence: 99%

Psychroflexus aurantiacus sp. nov., isolated from soil in the Yellow River Delta wetlands

Wang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, strictly aerobic, non-motile, orange-coloured bacterium, designated YR1-1T, was isolated from a soil sample collected from the Yellow River Delta wetlands (PR China). Growth was observed at a salinity of 1.0–15.0 % NaCl, 4–45 °C and pH 6.0–9.0. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that YR1-1T represented a member of the genus Psychroflexus , with the highest sequence similarity to Psychroflexus sediminis YIM-C238T (97.9 %), followed by Psychroflexus aestuariivivens (97.1 %) and Psychroflexus torquis (96.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between YR1-1T and other closely related type strains of species of the genus Psychroflexus were 68.7–86.3% and 17.8–30.9 %. The genome of the strain was 2 899 374 bp in length with 39.8 % DNA G+C content. The predominant fatty acids (>10 %) were iso-C15 : 0 and anteiso-C15 : 0. The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, phospholipid, diphosphatidylglycerol, two unidentified aminolipids and four unidentified lipids. The combined genotypic and phenotypic data indicate that YR1-1T represents a novel species within the genus Psychroflexus , for which the name Psychroflexus aurantiacus sp. nov., is proposed. The type strain is YR1-1T (=KCTC 72794T=CGMCC 1.17458T).

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Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions

Cited by 8 publications

References 37 publications

Cold Adaptation Mechanisms of a Snow Alga Chlamydomonas nivalis During Temperature Fluctuations

Cold Adaptation Mechanisms of a Snow Alga Chlamydomonas nivalis During Temperature Fluctuations

Cryopreservation of clonal and polyclonal populations of Chlamydomonas reinhardtii

Psychroflexus aurantiacus sp. nov., isolated from soil in the Yellow River Delta wetlands

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