Identification and classification of celery cultivars with RAPD markers

Abstract

Twenty-one celery (Apium graveolens L. var. dulce) cultivars, one celeriac (var. rapaceum) and one annual smallage (var. secalinum) cultivar were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with 28 arbitrary 10-mer primers. Among a total of 309 bands observed, 29 (9.3%) were polymorphic in the 23 cultivars screened, but only 19 (6.1%) markers were polymorphic within the 21 type dulce cultivars. These markers were sufficient to distinguish each of the cultivars used. The average marker difference was 6.4 between two celery cultivars, 16.7 between celery and annual smallage, 14.7 between celery and celeriac, and 12.0 between annual smallage and celeriac. The celery cultivars surveyed were classified into three groups based on the marker differences. The relationship among the dulce-type cultivars concluded from this research is basically consistent with the known lineage of the cultivars and the previous study using stem protein and isozyme markers. RAPD technology provides a new alternative for cultivar identification and classification in celery.

Morphological, Agronomical and Genetic Characterization of Egyptian Olive Clones Compared with the International Cultivars

“…The relationships among genotypes as revealed by dendrograms were done using SPSS windows (Version 10) program. DICE computer package was used to calculate the pair wise difference matrix and plot the phenogram among cultivars (Yang and Quiros, 1993).…”

Distinction between Cold-sensitive and -tolerant Jute by DNA Polymorphisms

“…These include the following: broccoli and cauliflower (Hu and Quiros 1991;Kresovich et al, 1992), cocoa (Wilde et al, 1992), banana (Kaemmer et al, 1992;Howell et al, 1994), papaya (Stiles et al, 1993), apple (Koller *To whom correspondence should be addressed. Tel: 88-2-966-1920-59 (6102);Fax: 88-2-861-5583 E-mail: haseena@bangla.net et al, 1993), celery (Yang and Quiros et al, 1993), onion (Wilkie et al, 1993), cranberry (Novy et al, 1994), sunflower (Lawson et al, 1994), rice (Takeuchi 1994), maize (Yazaki et al, 1994), Brassica juncea (Fujushiro et al, 1994), soybean (Lin, 1996;Powel, 1996), rose (Denbener et al, 1996), kenaf (Zhou et al, 1998;Cheng et al, 2000), roselle (Hanboonsong et al, 2000), and jute (Belayat et al, 2002).…”

DNA Fingerprinting of Jute Germplasm by RAPD

“…These include the following: broccoli and cauliflower (Hu and Quiros 1991;Kresovich et al, 1992), cocoa (Wilde et al, 1992), banana (Kaemmer et al, 1992;Howell et al, 1994), papaya (Stiles et al, 1993), apple (Koller et al, 1993), celery (Yang and Quiros et al, 1993), onion (Wilkie et al, 1993), cranberry (Novy et al, 1994), sunflower (Lawson et al, 1994), soybean (Powel 1996;Lin, 1996), rice (Takenchi, 1994), rose (Denbener et al, 1996), maize (Yazaki et al, 1994), and Brassica juncea (Fujushiro et al, 1994).…”