Identification and degradation of structural extracellular polymeric substances in waste activated sludge via a polygalacturonate-degrading consortium

Hu, Zhi-Yi; Lin, Yi-Peng; Wang, Qing-Ting; Zhang, Yixin; Tang, Jie; Hong, Sidi; Keino, Dai; Wang, Shuai; Lu, Yong-Ze; Loosdrecht, Mark C.M. van; Wu, Jianrong; Zeng, Raymond J.; Zhang, Fang

doi:10.1016/j.watres.2023.119800

Cited by 14 publications

(3 citation statements)

References 36 publications

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“…Simultaneously dosing alginate and nitrate should enrich a PCAN with higher nitrate removal rates over the long-term, and this will be examined in a further study. Hu et al demonstrated that a highly active polygalacturonate-degrading consortium (GDC) can promote EPS degradation in WAS fermentation via pectate lyases (EC 4.2.2.2) and exopectate lyase (4.2.2.9) . Thus, as shown in Figure , developing multiple hydrolyzing bacteria for WAS utilization could provide an alternative to achieving the goals of carbon neutrality and energy efficiency in WWTPs.…”

Section: Resultsmentioning

confidence: 99%

“…The final COD balance calculation was based on the COD of each metabolite concentration . The typical characteristics of the WAS, such as pH and total COD, were determined according to former works. , Three EPS layers (S-, LB-, and TB-EPS) were extracted from WAS and were filtered with a 0.45 μm microfiltration membrane. The activities of NAR and NIR of enriched PCAN were determined by NAR and NIR Assay kits (Solarbio, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

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Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation

Wang,

Tang,

Yan

et al. 2024

ACS EST Water

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Wastewater treatment plants (WWTPs) are being challenged to operate as carbon-neutral and energy-efficient systems. The structural extracellular polymeric substances (St-EPS) in waste-activated sludge (WAS), such as alginate, were revealed to form an architectural gel matrix, hindering the psychrophilic hydrolysis and acidogenesis and its application in nitrate removal. Consequently, volatile fatty acid (VFAs) production for nitrate removal at 15 °C is demonstrated by a novel, enriched psychrophilic consortium for alginate-degrading and nitrate-removing. Both alginate lyase (EC 4.2.2.3) and oligoalginate lyase (EC 4.2.2.26) are identified for alginate utilization by the metaproteomics analysis. A high nitrate removal rate of 7.6 (WAS as the substrate) to 13.0 (alginate as the substrate) mgN/(gVSS h) was achieved. By combining high-throughput and PacBio sequencing and metagenomics analysis, two genera of Bacteroides (69.4%) and Dysgonomons (12.8%) are revealed to enhance psychrophilic WAS fermentation. The VFAs can be utilized by denitrifiers for nitrate removal in an indirect pathway. Moreover, other genera of denitrifiers (Pseudomonas, 0.11%; and Massilla, 0.003%) can excrete alginate lyase, indicating the dual merits of St-EPS degradation and nitrate removal in the direct pathway. This work extends the application of WAS for denitrifiers, providing a new carbon-neutral and energy-efficient system in WWTPs, especially under psychrophilic conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation

Wang,

Tang,

Yan

et al. 2024

ACS EST Water

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“…Lü et al found that dosing with polygalacturonase (EC 3.2.1.15) increased the release of total polysaccharides in EPS by 7-fold. Recent results have shown that dosing with ADC or GDB can promote methane production. , Thus, these findings support the assumption that a higher content of genus Bacteroides in WAS may promote St-EPS hydrolysis and the release of organics, which is beneficial for subsequent acidogenesis and methanogenesis. Moreover, WAS treated with ABF exhibited a lower final St-EPS content than the control group, leading to higher methane production during WAS fermentation.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Dual Roles of an Alginate-Based Biodegradable Flocculant in Anaerobic Fermentation of Waste Activated Sludge: Dewaterability and Degradability

Wang,

Tang,

Ran

et al. 2024

Environ. Sci. Technol.

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Biodegradable flocculants are rarely used in waste activated sludge (WAS) fermentation. This study introduces an alginate-based biodegradable flocculant (ABF) to enhance both the dewatering and degradation of WAS during its fermentation. Alginate was identified in structural extracellular polymeric substances (St-EPS) of WAS, with alginate-producing bacteria comprising ∼4.2% of the total bacterial population in WAS. Owing to its larger floc size, higher contact angle, and lower free energy resulting from the Lewis acid-base interaction, the addition of the prepared ABF with a network structure significantly improved the dewaterability of WAS and reduced capillary suction time (CST) by 72%. The utilization of ABF by an enriched alginate-degrading consortium (ADC) resulted in a 35.5% increase in the WAS methane yield owing to its higher hydrolytic activity on both ABF and St-EPS. Additionally, after a 30 day fermentation, CST decreased by 62% owing to the enhanced degradation of St-EPS (74.4%) and lower viscosity in the WAS + ABF + ADC group. The genus Bacteroides, comprising 12% of ADC, used alginate lyase (EC 4.2.2.3) and pectate lyase (EC 4.2.2.2 and EC 4.2.2.9) to degrade alginate and polygalacturonate in St-EPS, respectively. Therefore, this study introduces a new flocculant and elucidates its dual roles in enhancing both the dewaterability and degradability of WAS. These advancements improve WAS fermentation, resulting in higher methane production and lower CSTs.

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Combining sulfite and freezing/thawing pretreatment to simultaneously improves methanogenesis, dewaterability, and pathogen inactivation of waste activated sludge: Depicting sulfite migration during ice crystal growth

Hou,

Qin,

Zuo

et al. 2024

Chemical Engineering Journal

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Identification and degradation of structural extracellular polymeric substances in waste activated sludge via a polygalacturonate-degrading consortium

Cited by 14 publications

References 36 publications

Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation

Uncovering the Nonnegligible Mechanism of Degrading Structural Extracellular Polymeric Substances for Nitrate Removal in Psychrophilic (15 °C) Waste-Activated Sludge Fermentation

Deciphering the Dual Roles of an Alginate-Based Biodegradable Flocculant in Anaerobic Fermentation of Waste Activated Sludge: Dewaterability and Degradability

Combining sulfite and freezing/thawing pretreatment to simultaneously improves methanogenesis, dewaterability, and pathogen inactivation of waste activated sludge: Depicting sulfite migration during ice crystal growth

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