Identification and detection of Macrophomina phaseolina by using species-specific oligonucleotide primers and probe

Babu, Bandamaravuri Kishore; Saxena, Anil Kumar; Srivastava, Alok Kumar; Arora, Dilip K.

doi:10.3852/mycologia.99.6.797

Cited by 69 publications

(66 citation statements)

References 22 publications

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“…This was the first report of M. phaseolina from hemp, turnip, okra, mungbean, marigold and Sonchus in Iran. The speciesspecific primers designed by Babu et al (2007) amplified one 350 bp band in all isolates of Macrophomina, confirming that the isolates obtained belonged to the species M. phaseolina. No bands were present in the negative controls, confirming that the primers were specific to M. phaseolina.…”

Section: Resultsmentioning

confidence: 70%

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Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Mahdizadeh¹,

Safaie²,

Goltapeh³

2011

The Plant Pathology Journal

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Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at 30°C and 37°C, average size of microsclerotia, and time to microsclerotia formation. The feathery colony phenotype was the most common (63.7%) on the chlorate selective medium and represented the chlorate sensitive phenotype of the Iranian Macrophomina phaseolina population. Meantime, inter simple sequence repeats (ISSR) Markers were used to assess the genetic diversity of the fungus. Unweighted pair-group method using arithmetic means (UPGMA) clustering of data showed that isolates did not clearly differentiate to the specific group according to the host or geographical origins, however, usually the isolates from the same host or the same geographic origin tend to group nearly. Our results did not show a correlation between the genetic diversity based on the ISSR and phenotypic characteristics. Similar to the M. phaseolina populations in the other countries, the Iranian isolates were highly diverse based on the phenotypic and the genotypic characteristics investigated and needs more studies using neutral molecular tools to get a deeper insight into this complex species.

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Section: Resultsmentioning

confidence: 70%

“…Isolates were identified to species using species-specific primers (MpKFI/MpKRI) according to Babu et al (2007). Controls included a no DNA negative control, DNA of isolates of Trichoderma harzianum, and Cytospora schusleri, Sclerotinia sclerotiorum.…”

Section: Open Accessmentioning

confidence: 99%

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Mahdizadeh¹,

Safaie²,

Goltapeh³

2011

The Plant Pathology Journal

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“…It was selected in a pathogenicity test conducted previously, in which no differences were observed among 22 isolates obtained from strawberry plants grown in San Pedro, Metropolitan region, Chile. To ensure a correct identification, the isolate Mp21.A was molecularly identified using the primers MpkF1 and MpkR1 [40]. For the greenhouse trial, the inoculum consisted in oat seeds infected with the plant pathogen.…”

Section: Inoculum Preparationmentioning

confidence: 99%

Susceptibility of strawberry cultivars to root and crown rot caused by Macrophomina phaseolina

Montejo‐Sánchez

Henríquez

Urcola

et al. 2016

JBR

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Abstract. BACKGROUND:A high incidence of root and crown rot of strawberries (Macrophomina phaseolina) has been observed in major strawberry production regions. In Chile, the pathogen was reported in strawberry plants in 2013. A strategy for disease management is the use of resistant cultivars. OBJECTIVE: The aim of this study was to evaluate the susceptibility of eleven strawberry cultivars to the disease. METHODS: Two trials were conducted. A first trial was performed under greenhouse conditions, with plants propagated by runners and inoculated by oat seeds infected with M. phaseolina. The impact of the disease on the growth of the plants and incidence were assessed. The second trial was performed in a growth chamber. Plants were obtained by in-vitro multiplication and were inoculated with a suspension of sclerotia of the same isolate. Plant mortality was assessed. RESULTS: Significant differences in susceptibility were observed among the eleven strawberry cultivars, with 'Florida Festival', 'Amiga' and 'Naiad' as the least susceptible cultivars, while 'Florida Fortuna' was the most susceptible cultivar in both trials. CONCLUSIONS: Differences were detected in terms of susceptibility of cultivars to root and crown rot of strawberry caused by M. phaseolina. This work contributes to knowledge about the susceptibility of strawberry cultivars to this disease.

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“…Little is known about the genetic complexity of M. phaseolina population in India or about the origin and spread of the pathogen i.e. whether it arose as a single lineage in one location or arose independently in several differentiation of M. phaseolina has been achieved using polymerase chain reaction (PCR) and hybridization based techniques [4][5][6][7][8]. The lack of strong correlation between genotype and geographical origin suggests a high level of diversity within and among the population of M. phaseolina analysis has many advantages as a means of characterizing genetic variability such as speed, low cost and minimal requirement for DNA.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Macrophomina phaseolina isolates from certain agro-climatic regions of India by using RAPD markers

et al. 2010

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Genetic diversity analysis of Macrophomina phaseolina isolates obtained from different host range and diverse geographical locations in India was carried out using used, primer OPB-08 gave the maximum polymorphism and the UPGMA clustering could separate 50 isolates in to ten groups at more than 65% similarity level. The ten clusters correlated well with the geographical locations with exceptions for isolates obtained from Eastern and Western Ghats. There was a segregation of isolates from these two geographical locations in to two clusters thus, distributing 10 genotypes in to eight geographical locations. All the isolates M. phaseolina irrespective of their host and geographical origin, exhibited two representative monomorphic bands at 250 bp and 1 kb, presence of these bands suggests that isolates might have evolved from a common ancestor but due to geographical isolation fallowed by natural selection and genetic drift might have segregated in to subpopulations. dispersal of single lineage in all locations in India.

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Identification and detection of Macrophomina phaseolina by using species-specific oligonucleotide primers and probe

Cited by 69 publications

References 22 publications

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran

Susceptibility of strawberry cultivars to root and crown rot caused by Macrophomina phaseolina

Genetic diversity of Macrophomina phaseolina isolates from certain agro-climatic regions of India by using RAPD markers

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