IDENTIFICATION AND EFFECTS OF MIXED INFECTION OF  Potyvirus ISOLATES WITH  Cucumber mosaic virus IN CUCURBITS

Barbosa, G. da S.; Lima, José Albérsio de Araújo; Queiróz, M. A. de; Dias, R. de C. S.; Lima, Cristiano Souza

doi:10.1590/1983-21252016v29n429rc

Cited by 14 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Oklahoma, 18.9% of samples were found to be infected with at least two viruses among 869 cucurbit samples [ 6 ]. A high incidence of mixed infections involving PRSV-W, WMV, and ZYMV has also been reported previously in different studies around the world: in the USA [ 6 , 20 , 22 ], Iran [ 12 ], Spain [ 13 ], Brazil [ 42 ], Panama [ 14 ], Venezuela [ 43 ], South Africa [ 44 ], Ivory Coast [ 45 ], Argentina [ 18 ], India [ 16 ], and France [ 19 ]. However, the proportion of mixed infections involving these three viruses in this study was higher than that in previous studies.…”

Section: Discussionsupporting

confidence: 74%

“…They may also lead to virus–virus interactions within a host, resulting in new variants with novel features [ 47 ]. Mixed virus infections can be antagonistic [ 47 , 48 , 49 , 50 ], synergistic [ 42 , 51 , 52 , 53 ], or neutral [ 53 , 54 ]. Additionally, the suppression of post-transcriptional expression by one virus helps in the replication of another virus, thereby forming a synergistic association [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

2021

View full text Add to dashboard Cite

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

show abstract

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

2021

View full text Add to dashboard Cite

show abstract

“…Similar phenomenon was also reported by Barbosa et al (2016) in Brazil, in which mixed infection of CMV, WMV, ZYMV and PRSV on cucurbits showed a variety of symptoms in the field. In Java and Central Java, there have also been reported mix infections of viruses in Cucurbits.…”

Section: Presence Of CMV In Sample Crops Based On Tas Elisasupporting

confidence: 87%

“…The second possibility is that the crops were infected by other distinct cucurbits viruses. Many authors reported that cucurbits could be infected by many viruses, commonly CMV, PRSV, ZYMV, WMV and others both in single or mix infection that induce a variety of symptoms including symptoms of CMV infection such as mosaic, vein clearing, vein banding and malformation (Zitter and Murphy, 2009;Romay et al, 2014;Barbosa et al, 2016;Sydanmetsa and Mbanzibwa, 2016).…”

Section: Presence Of CMV In Sample Crops Based On Tas Elisamentioning

confidence: 99%

Occurrence and distribution of cucumber mosaic virus in cucurbits in Karanganyar, Central Java, Indonesia

Supyani¹,

Chandra²,

Rochman³

et al. 2017

Afr. J. Agric. Res.

View full text Add to dashboard Cite

Cucumber mosaic virus (CMV) is an important pathogen in agricultural crops which is spread throughout the world with a wide host range. In Indonesia, CMV is also an important pathogen that infect various high economic value crops. Karanganyar regency is a horticultural production centers in Central Java. Its foremost products are cucurbits incuding cucumber (Cucumis sativus), melon (Cucumis melo), watermelon (Citrullus lanatus), pumpkin (Cucurbita moschata), chayote (Sechium edule), angled luffa (Luffa acutangula) and bitter melon (Momordica charantia). Recently, cucurbits crops in the area suffered from disease with symptoms of virus infection, especially CMV, that is, mosaic, vein clearing, vein banding, malformation, etc. The disease is detrimental to farmers. This research aimed to determine whether the causal agent of disease in the crops is CMV. For that, the authors conducted a survey in the field, picked up sample crops showing symptoms, brought the sample crops to the laboratory, and checked for the presence of CMV by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA). The results showed that of the 50 sample crops including 7 species of cucurbits showing symptoms of virus infection, 12 were detected to be infected by CMV. CMV was distributed evenly in cucurbits in Karanganyar regency.

show abstract

“…The fruits are stunted and deformed, resulting in reduced yield and making them non-marketable (Spadotti et al, 2015). ZYMV is markedly present in the producing regions of Brazil; Barbosa et al (2016), in a survey in the Vale do Rio São Francisco, obtained 44% symptomatic plants with the presence of this virus.…”

mentioning

confidence: 99%

Zucchini lineages with levels of resistance to ZYMV and SqMV viruses

Beloti

Maciel

Alves

et al. 2021

Rev. Colomb. Cienc. Hortic.

View full text Add to dashboard Cite

Zucchini (Cucurbita pepo L.) is a horticultural plant species of great socioeconomic value in tropical countries such as Colombia and Brazil. The production of zucchini is qualitatively and quantitatively affected by many diseases, especially viruses belonging to the Potyvirus (Zucchini yellow mosaic virus - ZYMV) and Comovirus (Squash mosaic virus - SqMV) groups. The primary strategy to reduce the spread of potentially damaging plant viruses is the development of genotypes with genetic tolerance; however, there are not many zucchini genotypes with multiple tolerance. Therefore, this study evaluated 66 zucchini genotypes to find sources of tolerance to the ZYMV and SqMV viruses. This experiment was conducted in a completely randomized design using genotypes from the germplasm bank of the Federal University of Uberlândia, including the genotypes: Emanuela (common commercial genotype) ‘Tronco Caserta’ (susceptible genotype) and PX 13067051 (resistant genotype). Leaf extracts containing viral particles were used as inoculant, and the distribution of grades of tolerance was recorded at the seedling stage. The lineages UFU-C×UFU-A#18#3;1, UFU-C×UFU-F#19#11;3, UFU-F#4#9;1, and UFU-D×UFU-F#7#21;1 and the Emanuela cultivar are alternatives for the production of new zucchini genotypes or hybrids with tolerance to the viruses ZYMV and SqMV. More severe symptoms were observed, as well as a larger number of susceptible genotypes for the ZYMV virus, indicating that this virus has great potential for causing damage and losses to zucchini crops.

show abstract

IDENTIFICATION AND EFFECTS OF MIXED INFECTION OF Potyvirus ISOLATES WITH Cucumber mosaic virus IN CUCURBITS

Cited by 14 publications

References 17 publications

High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Occurrence and distribution of cucumber mosaic virus in cucurbits in Karanganyar, Central Java, Indonesia

Zucchini lineages with levels of resistance to ZYMV and SqMV viruses

Contact Info

Product

Resources

About