Identification and expression analysis of nascent polypeptide-associated complex alpha gene in response to immune challenges in Japanese flounder Paralichthys olivaceus

Li, Shuo; Chen, Xiaoli; Geng, Xin; Zhan, Wenbin; Sun, Jinsheng

doi:10.1016/j.fsi.2015.06.033

Cited by 21 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro immune challenge experiments in Japanese flounder HKMs and PBLs with the pathogen-associated molecular pattern molecules LPS and poly(I:C) were detailed in a previous study [25]. Briefly, overnight-cultured Japanese flounder HKMs and PBLs (5 × 10 6 cells/well) were treated with 20 μg/ml LPS or poly(I:C) (final concentration, dissolved in cell culture medium, Sigma-Aldrich) for 4, 8, 12, 24, 36 or 48 h. Cells without PAMP treatment served as the controls.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus)

Peng

et al. 2019

BMC Vet Res

Self Cite

View full text Add to dashboard Cite

BackgroundCaspases are a family of conserved intracellular cysteine-dependent aspartate-specific cysteine proteases that play important roles in regulating cell death and inflammation. Our previous study revealed the importance of the inflammatory caspase 1 gene in extracellular ATP-mediated immune signaling in Japanese flounder, Paralichthys olivaceus. To explore the potential roles of other caspases in P. olivaceus innate immunity, we extended our study by characterizing of the responses of four additional P. olivaceus caspase genes, termed JfCaspase 2, 3, 6 and 8, to inflammatory challenge and extracellular ATP stimulation.ResultsSequence analysis revealed that the domain structures of all the Japanese flounder caspase proteins are evolutionarily conserved. Quantitative real-time PCR analysis showed that the JfCaspase 2, 3, 6 and 8 genes were expressed ubiquitously but at unequal levels in all examined Japanese flounder normal tissues. In addition, the basal gene expression levels of JfCaspase 2, 3, 6 and 8 were higher than those of JfCaspase 1 in both Japanese flounder head kidney macrophages (HKMs) and peripheral blood leukocytes (PBLs). Furthermore, immune challenge experiments showed that the inflammatory stimuli LPS and poly(I:C) significantly modulated the expression of the JfCaspase 2, 3, 6 and 8 genes in Japanese flounder immune cells. Finally, DNA fragmentation, associated with increased extracellular ATP-induced JfCaspase 2, 3, 6 and 8 gene expression and enzymatic activity, was inhibited by the caspase inhibitor Z-VAD-FMK in the HKMs.ConclusionOur findings demonstrate broad participation of multiple caspase genes in response to inflammatory stimulation in Japanese flounder immune cells and provide new evidence for the involvement of caspase(s) in extracellular ATP-induced apoptosis in fish.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus)

Peng

et al. 2019

BMC Vet Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous study also observed that transcription and secretion of IFN-g, IL-17, and IL-22 were increased in NACA-stimulated human peripheral blood mononuclear cells [11]. In addition, NACA has been identified as an immune response gene involved in innate immune regulation in Japanese flounder Paralichthys olivaceus [12]. Besides the important regulatory roles in innate immunity, NACA also is tightly linked to stress response.…”

Section: Introductionmentioning

confidence: 82%

“…Because NACA can interact and cooperate with several major chaperones to form functional networks to maintain proteostasis [21], the up-regulation of EsNACA upon environmental stress and immune challenges therefore indicated a potential role of EsNACA in promoting the proper folding of proteins and/or preventing the aggregation of nascent or damaged proteins under the stress and pathological conditions. Since NACA has been suggested a regulatory role in modulating innate immune-related gene expression in Japanese flounder [12], EsNACA may also function as a transcription factor in modulating stress adaptation and/or immune response involved gene expression. These hypotheses will be examined in the future study.…”

Section: Esnaca Expression In Response To Immune Challengesmentioning

confidence: 99%

Identification and characterization of nascent polypeptide-associated complex alpha from Chinese mitten crab (Eriocheir sinensis): A novel stress and immune response gene in crustaceans

Peng

Chen

et al. 2016

Fish & Shellfish Immunology

Self Cite

View full text Add to dashboard Cite

“…The relative gene expression levels of Trpm4 in Japanese flounder tissues, including blood, gill, head kidney, trunk kidney, liver, skin, muscle, intestine and spleen, were analysed by real‐time quantitative PCR (RT‐qPCR) as described in our previous study (Li, Wang, et al., 2020). The in vitro inflammatory challenge and in vivo bacterial infection experiments were performed as described previously (Li et al., 2015; Li, Wang, et al., 2020). The expression of Trpm4 in response to inflammatory stimulation and Edwardsiella tarda infection was assessed by RT‐qPCR as described below.…”

Section: Methodsmentioning

confidence: 99%

Identification and characterization of Trpm4 gene involved in regulating Japanese flounder (Paralichthys olivaceus) inflammatory response

Feng

Zhang

et al. 2021

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

The transient receptor potential (TRP) melastatin 4 (TRPM4) is a widely expressed Ca2+‐impermeable cation channel involved in modulating inflammatory and immune responses in mammals. However, the role of TRPM4 channel in fish immunity remains unclear. In this report, from a comparative immunological point of view, we identified and characterized a Trpm4 gene from Japanese flounder (Paralichthys olivaceus) and analysed its potential role in regulating the fish inflammatory response. The Japanese flounder Trpm4 gene is expressed in a wide range of tissues and encodes a 1264‐amino acid protein which expresses on the cell surface and shares several conserved domains with its mammalian counterparts. In vitro inflammatory challenge and in vivo bacterial infection experiments revealed that Japanese flounder Trpm4 expression was significantly modulated following different immune challenges, indicating the implication of Trpm4 in the fish immune response. Overexpression of TRPM4 significantly attenuated LPS‐ and poly(I:C)‐induced pro‐inflammatory cytokine expression in Japanese flounder FG‐9307 cells. In contrast, pharmacological inhibition of the endogenous TRPM4 channel activity in Japanese flounder head kidney macrophages resulted in increased pro‐inflammatory cytokine expression following LPS and poly(I:C) stimulations. Taken together, these findings indicate that TRPM4 channels may play a conserved role in regulating inflammatory response(s) in fish.

show abstract

Identification and expression analysis of nascent polypeptide-associated complex alpha gene in response to immune challenges in Japanese flounder Paralichthys olivaceus

Cited by 21 publications

References 33 publications

Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus)

Characterization of the responses of the caspase 2, 3, 6 and 8 genes to immune challenges and extracellular ATP stimulation in the Japanese flounder (Paralichthys olivaceus)

Identification and characterization of nascent polypeptide-associated complex alpha from Chinese mitten crab (Eriocheir sinensis): A novel stress and immune response gene in crustaceans

Identification and characterization of Trpm4 gene involved in regulating Japanese flounder (Paralichthys olivaceus) inflammatory response

Contact Info

Product

Resources

About