Identification and expression analysis of cytochrome P450 genes in Plecoptera oculata (Lepidoptera: Noctuidae)

Abstract

Cytochrome P450 monooxygenases (CYPs) play an important role in the decomposition of insecticides. Plecoptera oculata Moore is a lepidopteran forestry pest that severely affects Dalbergia odorifera T. Chen. In this study, 27 putative CYP genes were identified in the transcriptome of larval P. oculata. Phylogenetic analysis showed that these genes are grouped into four clans, 15 families, and 22 subfamilies. The tissue‐specific expression profile for each gene was determined using real‐time reverse transcription‐quantitative PCR (RT‐qPCR), and the 27 genes were found to be ubiquitously expressed, mainly in the midgut, Malpighian tubules, and fat body. The expression profiles of all 27 PoCYP genes exposed to half the lethal concentration (LC50) of sendebao and lambda‐cyhalothrin were determined using RT‐qPCR. The results showed that six and twelve PoCYP genes were significantly down‐ and up‐regulated, respectively, by exposure to sendebao, and three and twelve PoCYP genes were significantly down‐ and up‐regulated, respectively, by exposure to lambda‐cyhalothrin. To the best of our knowledge, this study is the first to show the expression of CYP genes in P. oculata. Our results provide a basis for further investigation of the roles of PoCYP genes in insecticide detoxification and as potential targets for novel pesticides.

Functional roles of two novel P450 genes in the adaptability of Conogethes punctiferalis to three commonly used pesticides

“…Analyses of P450 gene expression patterns provide useful information on the potential roles of these genes in biological functions ( Lyu et al, 2020 ). Insect P450 genes have a diverse distribution.…”