Identification and expression analysis of goose-type lysozyme in half-smooth tongue sole (Cynoglossus semilaevis)

Analysis of Two Lysozyme Genes and Antimicrobial Functions of Their Recombinant Proteins in Asian Seabass

“…The up-regulation of mRNA varied between the c-type and g-type lysozymes under the same treatment conditions and tissue types, suggesting different functions between these two genes. Similar up-regulation in the expression of these two genes after the bacterial challenge were reported in a number of fish species, such as orange-spotted grouper [11] , Japanese flounder [14] and half-smooth tongue sole [48]. The expression of the c-type lysozyme was up-regulated by different bacterial stimuli in the kidney of the orange-spotted grouper [49], however no significant change was observed in brill after injection of LPS or bacteria [2].…”

Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection

“…V. anguillarum is a type of Gram-negative bacteria that can cause severe epidemic vibriosis with symptoms of hemorrhage septicemia. Previous reports on immune analysis in C. semilaevis are concentrated on single gene, such as TLR9 [31], Interleukin-8 [32], chemokines such as CsCXCe1 [33] and CsCCK1 [34], MHC IIA and IIB [35] and goose-type lysozyme [36]. Although the whole genome reference [37] and some transcriptome information in C. semilaevis are now available [38], the understanding of the molecular mechanism against V. anguillarum infection is still deficient, thereby it is difficult to establish the measures to control the V. anguillarum disease.…”

Recombinant goose-type lysozyme in channel catfish: Lysozyme activity and efficacy as plasmid DNA immunostimulant against Aeromonas hydrophila infection

“…Ubiquitous expression of Lys-g in different tissues has been previously reported in various fish species, including Atlantic salmon (S. salar) [20], orange-spotted grouper (E. coioides) [27], half-smooth tongue sole (Cynoglossus semilaevis) [28], rock bream (Oplegnathus fasciatus) [29], and Atlantic cod (Gadus morhua) [30]. After exposure to A. hydrophila by bath immersion, CC-Lys-g was significantly up-regulated in all fish tissues tested.…”