Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta

Niu, Donghong; Wang, Lie; Bai, Zhiyi; Xie, Shumei; Zhao, Hongling; Li, Jiale

doi:10.1016/j.gene.2014.11.020

Cited by 28 publications

(12 citation statements)

References 33 publications

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“…Myostatin is a negative regulator of muscle growth in vertebrates, and Wang et al (2010) found that polymorphism of the myostatin gene was correlated with differential growth traits in mammals. In bivalves, myostatin have been suggested to have alternative functions that are related with cell development (Saina and Technau, 2009;Núñez-Acuña and Gallardo-Éscarate, 2014;Morelos et al 2015;Niu et al 2015). Insulin-like peptides have been reported to act as growth regulators of soft tissues and shell in bivalves (Taylor et al 1996;Gricourt et al 2003), and their roles in determining interindividual growth rate differences in bivalves have been recently suggested by Saavedra et al (2017), who found a significant overexpression of NOV-like protein in the gills of fastgrowing Ruditapes decussatus.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

et al. 2019

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The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast-and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas biosynthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system (leucine-rich repeat protein and galectin), along with genes involved in the response to cellular stress (Heat shock protein (HSP24) and metalloendopeptidase) as well as anaerobic metabolism (C4-dicarboxylate transporter). These results might suggest that S individuals would have a greater prevalence of pathogens/diseases or a higher susceptibility to the pathogens. Highlights ► Fast-and slow-growing mussel (Mytilus galloprovincialis) spat were found to differentially express 117 genes in gill tissue. ► Fast-growing mussels overexpressed genes involved in response to stimulus,growth and cell activity processes. ► Slow-growing mussels overexpressed genes involved in immune and defence processes. ► Feeding above or below the pseudofaeces production threshold did not exert a relevant effect on the gill transcriptome.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

et al. 2019

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“…However, despite recent fast developments in artificial breeding and aquaculture, new varieties of razor clams for artificial breeding are still severely lacking. At present, limited research has reported on growth-related genes in S. constricta , including IGFBP ( Xie et al, 2015 ), MSTN ( Niu et al, 2015 ), and GRB2 ( Zhao et al, 2018 ). There is thus a need to study growth-related genes and carry out molecular breeding of high-yield new varieties to support the sustainable development of the clam aquaculture industry.…”

Section: Introductionmentioning

confidence: 99%

A Potential Role of Bone Morphogenetic Protein 7 in Shell Formation and Growth in the Razor Clam Sinonovacula constricta

et al. 2020

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Bone morphogenetic proteins (BMPs) not only play essential roles in bone development but also are involved in embryonic growth, organogenesis cell proliferation and differentiation. However, the previous studies on the functions of shellfish BMPs genes are still very limited. To better understand its molecular structure and biological function, BMP7 of the razor clam Sinonovacula constricta ( Sc-BMP 7) was cloned and characterized in this study. The full length of Sc-BMP7 is 2252 bp, including an open reading frame (ORF) of 1257 bp encoding 418 amino acids. The protein sequence included a signal peptide (1–32 aa), a prodomain (38–270 aa) and a TGF-β domain (317–418 aa). The quantitative expression of eleven adult tissues showed that Sc-BMP 7 was significantly higher expressed in the gill, foot, and mantle ( P < 0.05), but lower in hemocytes and hepatopancreas. In the early development stages, low expression was detected in the stages of unfertilized mature eggs, fertilized eggs, 4-cell embryos, blastula, gastrulae, whereas it increased after the stage of trochophore and demonstrated the highest expression in umbo larvae ( P < 0.01). In shell repair experiment, Sc-BMP 7 showed increasing expression level after 12 h. The higher expression of Sc-BMP 7 was detected while Ca 2+ concentration was reduced in seawater. After inhibiting Sc-BMP 7 expression using RNA interference (RNAi) technology, expression of Sc-BMP 7 mRNA and protein were significantly down-regulated ( P < 0.05) in the central zone of mantle (nacre formation related tissue) and the pallial zone of mantle (prismatic layer formation related tissue). Association analysis identified two shared SNPs in exon of Sc-BMP 7 gene from 246 individuals of two groups. These results indicated that BMP 7 might be involved in shell formation and growth. These results would contribute to clarify the role of Sc-BMP 7 in the regulation of growth and shell formation, and provide growth-related markers for molecular marker assisted breeding of this species.

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“…In Boer goat, Chinese Xuhuai white goat and Chinese Haimen goat, one SNP locus was identified and related to body weight and back fat thickness in the 3′‐flanking region of the BMP 2 gene (Fang et al., 2010). Six SNP loci were found in the coding sequence of the MSTN gene of razor clam ( Sinonovacula constricta ), and two of which were significantly related to its shell length (Niu et al., 2015). However, researches about the relationship between smad4 gene‐related mutations and animal growth traits are lacking.…”

Section: Introductionmentioning

confidence: 99%

Identification of three SNPs insmad4gene by tetra‐primer ARMS PCR and analysis of their associations with growth traits in gynogenetic populations ofCulter alburnus

Chi

Jia

et al. 2020

Aquac Res

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Three single nucleotide polymorphisms, c.1462C > T, c.1663G > T and c.2023G > A, in smad4 gene were isolated and typed in a normal population (meio‐G0), a first‐generation gynogenetic population (meio‐G1) and a second‐generation gynogenetic population (meio‐G2) in topmouth culter (Culter alburnus) by using the tetra‐primer amplification refractory mutation system (ARMS) PCR. The homozygosity of different loci in the three populations was analysed to study the efficiency of gene purification in this fish. Correlation of the three populations to seven growth traits (body length, body weight, body thickness, body height, total length, caudal peduncle length and caudal peduncle height) was estimated. Results showed that homozygosity and homozygosity rates of three SNP loci from meio‐G0 to meio‐G2 increased obviously, and the average homozygosity of c.1462C > T and c.1663G > T loci was 0.930 and 0.880 in meio‐G2 population, which was significantly higher than that in meio‐G0 population. In the meio‐G1 and meio‐G2 populations, correlation of seven growth traits with the CC genotype was significantly higher than that with the TT and TC genotypes in the c.1462C > T locus (p < .05). These results suggested that the CC genotype in the c.1462C > T locus could be an advantage mutation that stably affects the growth of different generations of this fish. It may be used as a potential molecular marker for gene‐assisted selection to improve a range of growth traits in topmouth culter.

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Identification and expression characterization of the myostatin (MSTN) gene and association analysis with growth traits in the razor clam Sinonovacula constricta

Cited by 28 publications

References 33 publications

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

A Potential Role of Bone Morphogenetic Protein 7 in Shell Formation and Growth in the Razor Clam Sinonovacula constricta

Identification of three SNPs insmad4gene by tetra‐primer ARMS PCR and analysis of their associations with growth traits in gynogenetic populations ofCulter alburnus

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