Identification and Expression Profiling of Circulating MicroRNAs in Serum of Cysticercus pisiformis-Infected Rabbits

Chen, Guoliang; Wang, Liqun; Liu, Tingli; Li, Yanping; Zhang, Shaohua; Li, Hong; Luo, Xuenong

doi:10.3390/genes12101591

Cited by 6 publications

(4 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Domestic rabbits belonging to the species Oryctolagus cuniculus are social animals and are descended from European animals (Owuor et al, 2019). It is well known that domestic rabbits are intended for meat consumption and are a valuable source of a number of nutrients, but may contain many different endoparasites, including Passalurus ambiguus (Abdel-Gaber et al, 2019), Trichostrongylus retortaeformis (Cattadori et al, 2019), Nematodirus leporis (Tizzani et al, 2020), Cysticercus pisiformis (Chen et al, 2021) and Eimeria spp. (Ladron de Guevara et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Endoparasites of rabbits (Oryctolagus cuniculus domesticus) in Southern Ukraine

et al. 2022

View full text Add to dashboard Cite

One of the main tasks of ecological parasitology at the present stage is to establish patterns of spread of invasive diseases of animals in the environment through a thorough epidemiological examination, as well as to determine the main directions and factors of spread of parasites in Ukraine. Among the many pathogens, endoparasites play a major role in a number of diseases in rabbits with increased morbidity and mortality. In the body of both wild and domestic rabbits several species of parasites that form a parasitocenosis can be localized. The latter have a pathogenic effect on organs and tissues, leading to reduced weight gain, premature slaughter and even mortality. The prevalence of endoparasitoses was studied in weaned rabbits 60 days of age, 120 days of age at fattening, 180 days of age – mating age and adults of 320 days of age to determine the extensiveness and intensity of the dominant invasion. A total of 720 head was studied, 180 animals from each age group. Three species of nematodes were recorded in the rabbits: Trichostrongylus retortaeformis (Zeder, 1800), Nematodirus leporis (Ransom, 1907) and Passalurus ambiguus (Rudolphi, 1819); the cestode Taenia pisiformis (Bloch, 1780); three species of Emeria: Eimeria stiedae (Lindermann, 1865), which parasitizes in the bile ducts of the liver and gallbladder, E. magna (Perard, 1925) and E. media (Kessel, 1929) – in the epithelial cells of the intestine. The prevalence of the infestation depends on the age of the animals. According to the data obtained, eimeriosis was one of the main parasitic diseases of the rabbits. Dominant invasions of Eimeria in the intestines of 60-day-old rabbits were found in the studied animals, their extensiveness reached 19.4%, while the spread of hepatic Eimeria was registered in 13.3% of the examined rabbits. The total infestation of weaned rabbits with helminths was 22.2%, in rabbits for fattening – 26.7%, and in rabbits of mating age and adults – 20.0% and 20.6%, respectively. Global climate change will change the distribution and dynamics of soil-borne helminthiases, but host immunity may also affect host-parasite interactions. Subsequent studies will be aimed at elucidating the effect of mono and mixed invasions on the body of rabbits. Updated data on helminthiasis will expand the screening strategy to maintain rabbit health and reduce economic losses.

show abstract

Section: Discussionmentioning

confidence: 99%

Endoparasites of rabbits (Oryctolagus cuniculus domesticus) in Southern Ukraine

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Additionally, research has identified egr‐miR‐2a‐3p as a potential diagnostic biomarker for cystic echinococcosis (CE) 17 . Previous studies have revealed that cestodes share the same miRNA let‐7 (let‐7‐5p), 18 which has been shown to be one of the highly expressed miRNAs in cestodes 19‐21 . In addition, the expression level of let‐7‐5p was significantly upregulated in the serum and liver of metacestodes‐infected animals and humans, 22‐24 suggesting its potential as a target molecule for diagnosing metacestodiasis.…”

Section: Introductionmentioning

confidence: 99%

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Wang,

Pu,

Liu

et al. 2024

The FASEB Journal

Self Cite

View full text Add to dashboard Cite

Metacestodiasis is an infectious disease caused by the larval stage of cestode parasites. This disease poses a serious health hazard to wildlife, livestock, and humans, and it incurs substantial economic losses by impacting the safety of the livestock industry, the quality of meat production, and public health security. Unfortunately, there is currently no available molecular diagnostic method capable of distinguishing cysticercus‐ and Echinococcus‐derived microRNAs (miRNAs) from other helminthes and hosts in the plasma of metacestode‐infected animals. This study aims to develop a specific, sensitive, and cost‐efficient molecular diagnostic method for cysticercosis and echinococcosis, particularly for early detection. The study developed a rolling circular amplification (RCA)‐assisted CRISPR/Cas9 detection method based on parasite‐derived miRNA let‐7‐5p. Using a series of dilutions of the let‐7 standard, the limit of detection (LOD) of the qPCR, RCA, and RCA‐assisted CRISPR/Cas9 methods was compared. The specificity of qPCR and CRISPR/Cas9 was evaluated using four artificially synthesized let‐7 standards from different species. A total of 151 plasma samples were used to evaluate the diagnostic performance. Additionally, the study also assessed the correlation between plasma levels of let‐7‐5p, the number of Taenia pisiformis cysticerci, and the weight of Echinococcus multilocularis cysts. The results demonstrated that the RCA‐assisted CRISPR/Cas9 assay could significantly distinguish let‐7 from cestodes and other species, achieving a LOD of 10 aM; the diagnostic sensitivity and specificity for rabbit cysticercosis and mouse E. multilocularis were 100% and 97.67%, and 100% and 100%, respectively. Notably, let‐7‐5p gradually increased in the plasma of T. pisiformis‐infected rabbits from 15 days post infection (dpi), peaked at 60 dpi, and persisted until 120 dpi. In E. multilocularis‐infected mice, let‐7‐5p gradually increased from 15 dpi and persisted until 90 dpi. Furthermore, the expression of let‐7‐5p positively correlated with the number of cysticerci and cyst weight. These results indicated that the let‐7‐5p‐based RCA‐assisted CRISPR/Cas9 assay is a sensitive and specific detection method that can be used as a universal diagnostic method for metacestodiasis, particularly for early diagnosis (15 dpi).

show abstract

“…Cysticercus pisiformis is a widely prevalent larval form of tapeworm that primarily parasitizes the liver, omentum, and mesenteries of lagomorphs and rodents, leading to the development of cysticercosis pisiformis ( 1 , 2 ). Among these hosts, rabbits are the most frequently affected and suffer significant consequences from cysticercosis pisiformis.…”

Section: Introductionmentioning

confidence: 99%

Cysticercus pisiformis-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits

Chen¹,

Pu²,

Wang³

et al. 2023

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Cysticercosis pisiformis, a highly prevalent parasitic disease worldwide, causes significant economic losses in the rabbit breeding industry. Previous investigations have identified a novel microRNA, designated as novel-miR1, within the serum of rabbit infected with Cysticercus pisiformis. In the present study, we found that C. pisiformis-derived novel-miR1 was released into the rabbit serum via exosomes. Through computational analysis using TargetScan, miRanda, and PITA, a total of 634 target genes of novel-miR1 were predicted. To elucidate the functional role of novel-miR1, a dual-luciferase reporter assay was utilized and demonstrated that novel-miR1 targets rabbit Toll-like receptor 2 (TLR2). Rabbit peripheral blood lymphocytes (PBLCs) were transfected with novel-miR1 mimic and mimic NC, and the in vitro experiments confirmed that novel-miR1 suppressed the expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 through the nuclear factor kappa B (NF-κB) pathway. In vivo experiments demonstrated that novel-miR1 was significantly upregulated during the 1–3 months following infection with C. pisiformis in rabbits. Notably, this upregulation coincided with a downregulation of TLR2, P65, pP65, TNF-α, IL-1β, and IL-6 in PBLCs. Collectively, these results indicate that the novel-miR1 derived from C. pisiformis inhibited the rabbits’ immune response by suppressing the NF-κB-mediated immune response. This immune modulation facilitates parasite invasion, survival, and establishment of a persistent infection.

show abstract

Identification and Expression Profiling of Circulating MicroRNAs in Serum of Cysticercus pisiformis-Infected Rabbits

Cited by 6 publications

References 45 publications

Endoparasites of rabbits (Oryctolagus cuniculus domesticus) in Southern Ukraine

Endoparasites of rabbits (Oryctolagus cuniculus domesticus) in Southern Ukraine

Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9

Cysticercus pisiformis-derived novel-miR1 targets TLR2 to inhibit the immune response in rabbits

Contact Info

Product

Resources

About