Identification and expression profiling of sugar transporter genes during sugar accumulation at different stages of fruit development in apricot

Iqbal, Shahid; Ni, Xiaopeng; Bilal, Muhammad Saqib; Shi, Ting; Khalil-Ur-Rehman, Muhammad; Pan, Zhijuan; Gao, Jie; Usman, Muhammad; Gao, Zhihong

doi:10.1016/j.gene.2020.144584

Cited by 27 publications

(27 citation statements)

References 50 publications

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“…RT‐qPCR was performed using the ABI‐7300 Real‐Time PCR system (Applied Biosystems, Foster City, CA, USA) and SYBR Green Master Mix (Toyobo, Osaka, Japan). The experiment was performed using the method described by (Guo et al., 2018a; Iqbal et al., 2020; Wu et al., 2019). Three technical repeats were performed for each biological repeat.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide analysis of PmTCP4 transcription factor binding sites by ChIP‐Seq during pistil abortion in Japanese apricot

Iqbal

Pan

et al. 2020

The Plant Genome

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The TCP4 transcription factor plays an important role in plant growth and development, especially in flower development. PmTCP4 is involved in the process of pistil abortion in Japanese apricot, but its molecular mechanism, particularly the DNA binding sites and co-regulatory genes, are quite unknown. Therefore, to identify the genome-wide binding sites of PmTCP4 transcription factors and their co-regulatory genes, chromatin immunoprecipitation sequencing (ChIP-Seq) was carried out. ChIP-Seq data produced the maximum enriched peaks in two Japanese apricot cultivars 'Daqiandi' (DQD) and 'Longyan' (LY), which showed that the majority of DNA-protein interactions are relevant and have a significant function in binding sites. Moreover, 720 and 251 peak-associated genes regulated by PmTCP4 were identified in DQD and LY, respectively, and most of them were involved in the flower and pistil development process. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that photosynthesis and oxidative phosphorylation were the most enriched pathways in both cultivars and all identified genes related to these pathways were downregulated. This study will provide a reference for a better understanding of the PmTCP4 regulatory mechanism during pistil abortion in Japanese apricot.

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Section: Methodsmentioning

confidence: 99%

Genome‐wide analysis of PmTCP4 transcription factor binding sites by ChIP‐Seq during pistil abortion in Japanese apricot

Iqbal

Pan

et al. 2020

The Plant Genome

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“…It worth mentioning that the rate of sorbitol translocation was faster in HH cultivar than KA which might be due to differences in source (leaves) to sink (fruit) ratios [16] or some other factors affecting phloem transport such as water status of the plant [17] and gene expression levels of sugar transporters [18] . Iqbal et al [18] . showed a strong positive correlation between ParSuSy5 , ParSuSy6 , ParSuSy7 , and ParFK1 genes and sugar accumulation during apricot fruit development and ripening.…”

Section: Discussionmentioning

confidence: 98%

“…In this essence, the lag phase observed in the sorbitol and sucrose contents of apricot fruits between 56 and 77 DAB in the present study might indicate that apricot leaves became fully mature only after 77 DAB and capable of exporting photosynthates to fruits. It worth mentioning that the rate of sorbitol translocation was faster in HH cultivar than KA which might be due to differences in source (leaves) to sink (fruit) ratios [16] or some other factors affecting phloem transport such as water status of the plant [17] and gene expression levels of sugar transporters [18] . Iqbal et al [18] .…”

Section: Discussionmentioning

confidence: 99%

Changes in Compositional Properties during Fruit Development and On‐Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars

Karabulut

Özdemir

Koc

et al. 2021

Chemistry & Biodiversity

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The objective of the present study was to investigate the variations in some major primary (sugars and organic acids) and secondary (phenolics, β‐carotene) metabolite contents during fruit development and ripening in two important apricot cultivars (Hacıhaliloğlu and Kabaaşı). The changes in the compositional properties of two apricot cultivars were monitored during fruit development with one‐week intervals from 56 to 119 days after blossom. During fruit development, the contents of organic acids and phenolics decreased whereas that of sucrose and sorbitol increased. p‐Coumaric acid was the only phenolic compound which increased in concentration during fruit development regardless of the cultivar. The content of the other phenolic compounds decreased in a cultivar‐dependent manner. The β‐carotene content of the cultivars showed distinct patterns of change such that 3 fold increase in β‐carotene content of Kabaaşı cultivar was observed whereas the β‐carotene content of the Hacıhaliloğlu cultivar did not show any significant change during fruit development.

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“…Gene sequences were extracted, and their primers were designed using Primer 3 software. RNA extraction and quality tests were performed as described above, and RT-qPCR was performed according to the method described by [30,31]. Using RPII as a reference internal gene, the expression levels of the genes were analyzed via the 2 − CT method [32].…”

Section: Quantitative Reverse Transcription Pcr For Data Validationmentioning

confidence: 99%

Analyzing Differentially Expressed Genes and Pathways Associated with Pistil Abortion in Japanese Apricot via RNA-Seq

Shi

Iqbal

Ayaz

et al. 2020

Genes

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Reproduction is a critical stage in the flower development process, and its failure causes serious problems affecting fruit quality and yield. Pistil abortion is one of the main factors in unsuccessful reproduction and occurs in many fruit plants. In Japanese apricot, the problem of pistil abortion is very common and affects fruit quality and plant yield; however, its molecular mechanism is not clearly understood. Therefore, in the current study, we used RNA-Seq to identify the differentially expressed genes (DEGs) and pathways actively involved in pistil abortion. A total of 3882 differentially expressed genes were found after cutoff and pairwise comparison analysis. According to KEGG pathway analysis, plant hormone signaling transduction and metabolic pathways were found most significantly enriched in this study. A total of 60 transcription factor families such as MADS-box, NAC and TCP showed their role in this process. RT-qPCR assays confirmed that the expression levels were consistent with RNA-Seq results. This study provides an alternative to be considered for further studies and understanding of pistil abortion processes in Japanese apricot, and it provides a reference related to this issue for other deciduous fruit crops.

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Identification and expression profiling of sugar transporter genes during sugar accumulation at different stages of fruit development in apricot

Cited by 27 publications

References 50 publications

Genome‐wide analysis of PmTCP4 transcription factor binding sites by ChIP‐Seq during pistil abortion in Japanese apricot

Genome‐wide analysis of PmTCP4 transcription factor binding sites by ChIP‐Seq during pistil abortion in Japanese apricot

Changes in Compositional Properties during Fruit Development and On‐Tree Ripening of Two Common Apricot (Prunus armeniaca L.) Cultivars

Analyzing Differentially Expressed Genes and Pathways Associated with Pistil Abortion in Japanese Apricot via RNA-Seq

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