Identification and Functional Analysis of Tomato TPR Gene Family

Abstract

Tomato (Solanum lycopersicum) as an important vegetable grown around the world is threatened by many diseases, which seriously affects its yield. Therefore, studying the interaction between tomato and pathogenic bacteria is biologically and economically important. The TPR (Tetratricopeptide repeat) gene family is a class of genes containing TPR conserved motifs, which are widely involved in cell cycle regulation, gene expression, protein degradation and other biological processes. The functions of TPR gene in Arabidopsis and wheat plants have been well studied, but the research on TPR genes in tomato is not well studied. In this study, 26 TPR gene families were identified using bioinformatics based on tomato genome data, and they were analyzed for subcellular localization, phylogenetic evolution, conserved motifs, tissue expression, and GO (Gene Ontology) analysis. The qRT-PCR was used to detect the expression levels of each member of the tomato TPR gene family (SlTPRs) under biological stress (Botrytis cinerea) and abiotic stress such as drought and abscisic acid (ABA). The results showed that members of the tomato TPR family responded to various abiotic stresses and Botrytis cinerea stress, and the SlTPR2 and SlTPR4 genes changed significantly under different stresses. Using VIGS (Virus-induced gene silencing) technology to silence these two genes, the silenced plants showed reduced disease resistance. It was also shown that TPR4 can interact with atpA which encodes a chloroplast ATP synthase CF1 α subunit. The above results provide a theoretical basis for further exploring the molecular mechanism of TPR-mediated resistance in disease defense, and also provide a foundation for tomato disease resistance breeding.

Transcriptomic analysis of the response of Avena sativa to Bacillus amyloliquefaciens DGL1

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A Combination of a Genome-Wide Association Study and a Transcriptome Analysis Reveals circRNAs as New Regulators Involved in the Response to Salt Stress in Maize

“…This gene encodes a tetratricopeptide repeat (TPR)-like superfamily protein, which is involved in the regulation of different cellular functions [ 32 , 33 ]. In tomatoes, TPR family genes were previously reported to respond to various biotic and abiotic stresses [ 34 ].…”

Association mapping for image‐based root traits in tropical maize under water stress in semi‐arid regions

“…These TPR domain‐containing proteins are part of multisubunit protein complexes that regulate transcription, transport, photosynthesis of the photosynthetic system, and protein folding and perform diverse functions during plant development and drought stress (Wei & Han, 2017). In tomatoes, for example, TPR family genes have been reported to respond to various biotic and abiotic stresses (Zhou et al., 2021).…”