Identification and functional analysis of a non-hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus.

Heldens, J.G.M.; Broer, R.; Zuidema, D.; Goldbach, Rob; Vlak, Just M.

doi:10.1099/0022-1317-78-6-1497

Cited by 36 publications

(51 citation statements)

References 44 publications

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“…5, lane 4), suggesting that the DNA replication is hr-specific. These results contrast with the observation that the SeMNPV non-hr ori was able to replicate to a significant level in the presence of AcMNPV as helper virus (Heldens et al, 1997).…”

Section: Specificity Of Sehrs In Dna Replicationcontrasting

confidence: 56%

“…Only one copy of such a non-hr ori was found in the AcMNPV genome (Kool et al, 1994 ;Lee & Krell, 1994). Non-hr oris have also been identified in the genomes of OpMNPV and Spodoptera exigua MNPV (SeMNPV) (Pearson et al, 1993 ;Heldens et al, 1997). Enhancing activity of non-hrs has not been demonstrated yet.…”

Section: Introductionmentioning

confidence: 99%

“…The assay to test the replicative ability of the Sehr and Achr plasmids was as previously described by Heldens et al (1997) for the SeMNPV non-hr. In short, 10' Sf-AE-21 cells were transfected with 1 µg SeMNPV or AcMNPV hr-containing plasmids and infected 16 h later with the respective MNPVs with a m.o.i.…”

Section: Methodsmentioning

confidence: 99%

“…Sf-AE-21 cells transfected with pSeEcoRI-2.2 (lane 1) and pUC19 (all lanes) alone were included in the assay in order to identify the background of input DNA for DpnI digestions. All hr-containing SeMNPV fragments (lanes 2-7) replicated at a lower level than the non-hr type ori present on pSeXbaI-F2 (lane 11) (Heldens et al, 1997). Based on the replicative ability of pSeCHK-5.7 (Sehr4, lane 4) and of pSeXbaI-H (Fig.…”

Section: Motifmentioning

confidence: 99%

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Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication.

Broer

Heldens

Strien

et al. 1998

Journal of General Virology

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Section: Specificity Of Sehrs In Dna Replicationcontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Motifmentioning

confidence: 99%

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Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication.

Broer

Heldens

Strien

et al. 1998

Journal of General Virology

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“…Although no single homologous repeat region is essential for DNA replication of AcMNPV (Carstens & Wu, 2007). A second type of replication origin, a non-hr ori, has been identified in several NPVs (Habib & Hasnain, 2000;Heldens et al, 1997;Kool et al, 1994). These complex structures are composed of multiple direct and inverted repeats within a region spanning up to 4000 bp and are present only once per genome.…”

Section: Homologous Regions (Hrs)mentioning

confidence: 99%

Genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, Adoxophyes orana

Hilton

Winstanley

2008

Journal of General Virology

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Adoxophyes orana nucleopolyhedrovirus (AdorNPV) was isolated from overwintering larvae from an orchard in the UK. The nucleotide sequence of the AdorNPV DNA genome was determined and analysed. The genome contains 111724 bp and has a G+C content of 35.0 mol%. The analysis predicted 121 ORFs of 150 nt or larger. Of these putative genes, 118 were homologous to genes identified previously in the Adoxophyes honmai nucleopolyhedrovirus (AdhoNPV) genome (83.3-100 % aa identity), and three AdorNPV ORFs were unique. There were four small homologous regions that consisted of a similar core sequence and at the same relative positions in the genome as AdhoNPV, but they differed in the number of repeats and orientation. Some genes that have been reported to have major roles in baculovirus biology were either absent or truncated in the AdorNPV genome. These included chitinase, which is involved in the liquefaction of the host, and the C-terminal of the ecdysteroid UDP-glucosyltransferase (egt) protein, which was truncated by 149 aa compared with AdhoNPV, with essential amino acids absent. The AdorNPV genome encoded two inhibitor of apoptosis (iap) genes compared with three in AdhoNPV and three bro genes compared with four in AdhoNPV. The susceptibility of A. orana larvae to AdorNPV was evaluated in laboratory bioassays using inoculation by microdroplet feeding and applied dose assays. LD 50 for neonates was 56 occlusion bodies rising to 2.3¾10 4 for fifth instar larvae. Median survival time values using an LD 80 dose were 8.8 days for neonates and 7.0 days for fifth instar larvae. INTRODUCTIONThe family Baculoviridae consists of invertebrate viruses with large circular, covalently closed, double-stranded DNA genomes with enveloped nucleocapsids and an occluded form of the virus embedded in proteinaceous occlusion bodies (OBs) . There are two genera of baculovirus, the Nucleopolyhedrovirus (NPV) and the Granulovirus (GV) based on OB morphology . The NPVs are further classed into group I and II NPVs (Bulach et al., 1999;Zanotto et al., 1993). The majority of the sequenced NPVs infect insects of the Lepidopteran order, but NPVs that infect dipteran or hymenopteran insect species like the mosquito-infecting Culex nigripalpus NPV, or the NPVs from three Neodiprion species, have been sequenced (Afonso et al., 2001;Garcia-Maruniak et al., 2004;Lauzon et al., 2004; Duffy et al., 2006). Baculoviruses are used worldwide as biological control agents of agricultural and forest pests. The summer fruit tortrix moth (Adoxophyes orana) is a pest of apples and pears in most of Europe and Japan.During the past 25 years, there has been increased interest in the use of a naturally occurring GV as a control agent for the summer fruit tortrix (Cross et al., 1999). Recently, a mixture of baculoviruses was recovered from larvae in the UK (Hilton & Winstanley, 2008). These were separated and found to comprise an NPV and a GV. The GV (Adoxophyes orana GV) has been characterized and completely sequenced (Wormleaton et al., 2003;Hilton & Winstanley, 2...

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Sequence and Organization of the Mamestra configurata Nucleopolyhedrovirus Genome

Donly

et al. 2002

Virology

110

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The nucleotide sequence of the genome of the nucleopolyhedrovirus (NPV) from Mamestra configurata (MacoNPV, isolate 90/2), a group II NPV, was determined and analyzed. The MacoNPV DNA genome consists of 155,060 bp and has an overall G+C content of 41.7%. Computer-assisted analysis predicted 169 open reading frames (ORFs) of 150 nucleotides or greater that showed minimal overlap. BLAST searches and comparisons with completely sequenced baculoviruses indicated that there were 66 ORFs conserved among the nine baculoviruses compared and an additional 17 ORFs were conserved among the NPVs. The gene content and gene arrangement in MacoNPV were most similar to those of SeMNPV, including two putative odv-e66 and p26 gene homologues. However, in contrast to SeMNPV, 8 ORFs with homology to baculovirus repeat ORFs (bro) and single copies of enhancin and conotoxin-like protein ORFs were found in MacoNPV. The MacoNPV genome contained four homologous regions, each with 10 to 17 repeated sequences. Each repeat was 60 to 86 nucleotides in length and contained an approximately 43-bp-long imperfect palindrome. There were 13 ORFs unique to MacoNPV, ranging from a small ORF of 196 bp to larger ORFs of up to 1047 bp, and many of these contained typical early and late baculovirus consensus promoters.

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Identification and functional analysis of a non-hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus.

Cited by 36 publications

References 44 publications

Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication.

Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication.

Genomic sequence and biological characterization of a nucleopolyhedrovirus isolated from the summer fruit tortrix, Adoxophyes orana

Sequence and Organization of the Mamestra configurata Nucleopolyhedrovirus Genome

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