Identification and functional analysis of the Mandarin fish (Siniperca chuatsi) hypoxia-inducible factor-1α involved in the immune response

Yu, Yang; Qin, Xiao-Wei; Zeng, Ruoyun; Wang, Yuan‐Yuan; Li, Zhimin; Mi, Shu; Weng, Shaoping; Guo, Chang-Jun; He, Jianguo

doi:10.1016/j.fsi.2019.04.298

Cited by 32 publications

(13 citation statements)

References 34 publications

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“…Mandarin fish ( Siniperca chuatsi ) is an economically important species that is widely distributed in China; its industry is threatened by different aquatic viruses, especially the Infectious spleen and kidney necrosis virus (ISKNV) [ 11 ]. Iridoviruses are large dsDNA viruses that can infect both invertebrates (particularly insects and crustacean), and poikilothermic vertebrates (fish, amphibians, and reptiles), leading to systematic diseases [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Chen

Wang

et al. 2021

IJMS

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Exosomes are associated with cancer progression, pregnancy, cardiovascular diseases, central nervous system-related diseases, immune responses and viral pathogenicity. However, study on the role of exosomes in the immune response of teleost fish, especially antiviral immunity, is limited. Herein, serum-derived exosomes from mandarin fish were used to investigate the antiviral effect on the exosomes of teleost fish. Exosomes isolated from mandarin fish serum by ultra-centrifugation were internalized by mandarin fish fry cells and were able to inhibit Infectious spleen and kidney necrosis virus (ISKNV) infection. To further investigate the underlying mechanisms of exosomes in inhibiting ISKNV infection, the protein composition of serum-derived exosomes was analyzed by mass spectrometry. It was found that myxovirus resistance 1 (Mx1) was incorporated by exosomes. Furthermore, the mandarin fish Mx1 protein was proven to be transferred into the recipient cells though exosomes. Our results showed that the serum-derived exosomes from mandarin fish could inhibit ISKNV replication, which suggested an underlying mechanism of the exosome antivirus in that it incorporates Mx1 protein and delivery into recipient cells. This study provided evidence for the important antiviral role of exosomes in the immune system of teleost fish.

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Section: Introductionmentioning

confidence: 99%

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Chen

Wang

et al. 2021

IJMS

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“…Hypoxia-inducible factors (HIFs) are recognized as master regulators of the cellular response to hypoxia stress by binding HRE (hypoxiaresponsive element) (Schofield and Ratcliffe, 2004;Choudhry and Harris, 2018). The role of scHIF-1α in the regulation of the HIF signaling pathway and the immune response was confirmed in mandarin fish (Siniperca chuatsi) (He et al, 2019). Cancer magister responds to hypoxia by increasing Hc concentrations and regulating subunit compositions (Head, 2010).…”

Section: Introductionmentioning

confidence: 99%

Air Exposure Affects Physiological Responses, Innate Immunity, Apoptosis and DNA Methylation of Kuruma Shrimp, Marsupenaeus japonicus

Wang

et al. 2020

Front. Physiol.

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Air exposure stress is a common phenomenon for commercial crustacean species in aquaculture and during waterless transportation. However, the antioxidant responses to air exposure discussed in previous studies may be insufficient to present the complexities involved in this process. The comprehensive immune responses, especially considering the immune genes, cell apoptosis, and epigenetic changes, are still unknown. Accordingly, we investigated the multifaceted responses of Marsupenaeus japonicus to air exposure. The results showed that the expression profiles of the apoptosis genes (e.g., IAP, TXNIP, caspase, and caspase-3) and the hypoxiarelated genes (e.g., hsp70, hif-1α, and HcY) were all dramatically induced in the hepatopancreas and gills of M. japonicus. Heart rates, T-AOC (total antioxidant capacity) and lactate contents showed time-dependent changes upon air exposure. Air exposure significantly induced apoptosis in hepatopancreas and gills. Compared with the control group, the apoptosis index (AI) of the 12.5 h experimental group increased significantly (p < 0.05) in the hepatopancreas and gills. Most individuals in the experimental group (EG, 12.5 h) had lower methylation ratios than the control group (CG). Air exposure markedly reduced the full-methylation and total-methylation ratios (31.39% for the CG and 26.46% for the EG). This study provided a comprehensive understanding of the antioxidant responses of M. japonicus considering its physiology, innate immunity, apoptosis, and DNA methylation levels, and provided theoretical guidance for waterless transportation.

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“…In addition, there was a notable increase in the expression of MTOR, HIF1A, IL1B, MYC, and SLC2A1 on day 45 after bathing in water containing Poly I:C. The dependence of the mechanistic target of rapamycin (MTOR) on TLR3-induced cell activation has been shown in human keratinocytes [98]. Moreover, HIF1A has been found to be highly expressed during Poly I:C stimulation in mandarin fish (Siniperca chuatsi) [99]. MYC (and HIF1A) has previously been found to be highly expressed in human breast cancer cells treated with Poly I:C [100].…”

Section: Metabolic Genesmentioning

confidence: 98%

Immunostimulant Bathing Influences the Expression of Immune- and Metabolic-Related Genes in Atlantic Salmon Alevins

Figueiredo

Kristoffersen

Bhat

et al. 2021

Biology

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Disease resistance of fish larvae may be improved by bath treatment in water containing immunostimulants. Pattern recognition receptors, such as TLR3, TLR7, and MDA5, work as an “early warning” to induce intracellular signaling and facilitate an antiviral response. A single bath of newly hatched larvae, with Astragalus, upregulated the expression of IFNα, IFNc, ISG15, MDA5, PKR, STAT1, TLR3, and TLR7 immune genes, on day 4 post treatment. Similar patterns were observed for Hyaluronic acid and Poly I:C. Increased expression was observed for ISG15, MDA5, MX, STAT1, TLR3, TLR7, and RSAD2, on day 9 for Imiquimod. Metabolic gene expression was stimulated on day 1 after immunostimulant bath in ULK1, MYC, SLC2A1, HIF1A, MTOR, and SIX1, in Astragalus, Hyaluronic acid, and Imiquimod. Expression of NOS2 in Poly I:C was an average fourfold above that of control at the same timepoint. Throughout the remaining sampling days (2, 4, 9, 16, 32, and 45 days post immunostimulant bath), NOS2 and IL1B were consistently overexpressed. In conclusion, the immunostimulants induced antiviral gene responses, indicating that a single bath at an early life stage could enable a more robust antiviral defense in fish. Additionally, it was demonstrated, based on gene expression data, that cell metabolism was perturbed, where several metabolic genes were co-regulated with innate antiviral genes.

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Identification and functional analysis of the Mandarin fish (Siniperca chuatsi) hypoxia-inducible factor-1α involved in the immune response

Cited by 32 publications

References 34 publications

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein

Air Exposure Affects Physiological Responses, Innate Immunity, Apoptosis and DNA Methylation of Kuruma Shrimp, Marsupenaeus japonicus

Immunostimulant Bathing Influences the Expression of Immune- and Metabolic-Related Genes in Atlantic Salmon Alevins

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