Identification and Functional Analysis of a Novel Hydrophobic Protein VdHP1 from Verticillium dahliae

Zhang, Xiaojian; Zhao, Lihong; Liu, Shichao; Zhou, Jinglong; Yu, Wu; Feng, Zili; Zhang, Yalin; Zhu, Heqin; Wei, Feng; Feng, Hongjie

doi:10.1128/spectrum.02478-21

Cited by 15 publications

(21 citation statements)

References 82 publications

(102 reference statements)

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“…Concomitant with impaired dispersal, scanning electron microscopy analysis of the Δ sep and Δ hfbA mutants showed a morphological change in spores and conidiophore structure compared to the control ( Fig. 5 ), as has been reported in studies of other fungi ( 5 , 28 ). It is likely that structural changes in Δ sep and Δ hfbA spores contributed to the reduced dispersion.…”

Section: Discussionsupporting

confidence: 78%

“…Our greatest interest was assessing the fitness of the Δ sep mutant in host colonization because several hydrophobin mutants have been reported as being less virulent than other plant-pathogenic fungi, including F. graminearum , M. oryzae , Ophiostoma novo-ulmi , Verticillium dahliae , and Cryphonectria parasitica ( 7 , 8 , 14 , 28 , 29 ). The most powerful method for assessing the fitness of different fungal strains is through competition experiments ( 30 – 34 ).…”

Section: Discussionmentioning

confidence: 99%

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The Hydrophobin Gene Family Confers a Fitness Trade-off between Spore Dispersal and Host Colonization in Penicillium expansum

Luciano-Rosario

Eagan

Aryal

et al. 2022

mBio

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

The Hydrophobin Gene Family Confers a Fitness Trade-off between Spore Dispersal and Host Colonization in Penicillium expansum

Luciano-Rosario

Eagan

Aryal

et al. 2022

mBio

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“…Therefore, it is particularly important to study the molecular mechanism of V. dahliae and host plants. In previous studies, knockout of FgERG4 in F. graminearum led to a decline in conidial production and malformation of conidia (Liu, et al 2013), knockout of VdHP1 in V. dahliae reduces conidial smoothness (Zhang, et al 2022a). In this study, it was found that the knockout of VdERG2 led to the decrease of conidial yield and conidial germination rate, and abnormal conidial morphology (Figure 2).…”

Section: Discussionmentioning

confidence: 88%

“…For the determination of abiotic stress, 1 mol/L KCl, 1 mol/L NaCl, 1 mol/L Sorbitol, 0.004% SDS and 0.02% CR were added to PDA medium (Liu, et al 2021a, Zhang, et al 2022a). Normal PDA medium was used as a control.…”

Section: Stress Response and Carbon Utilization Detectionmentioning

confidence: 99%

“…In order to study the role of VdERG2 in the growth of V. dahliae during the absorption or utilization of speci c carbon sources, sucrose (30 g/L), cellolose (5 g/L), skim milk (18 g/L), pectin (10 g/L) and starch (17 g/L) were added to Czapek-Dox medium without sucrose, respectively. The colony diameter of all strains were measured after 14 days of culture (Guo, et al 2022, Liu, et al 2021c, Zhang, et al 2022a, Zhang, et al 2015. All experiments were repeated three times.…”

Section: Stress Response and Carbon Utilization Detectionmentioning

confidence: 99%

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VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae

Liu

Zhang

et al. 2022

Curr Genet

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The ergosterol biosynthesis pathway plays an important role in model pathogenic bacteria Saccharomyces cerevisiae, but little is known about the biosynthesis of ergosterol in pathogenic fungus Verticillium dahliae. In this study, we identi ed the VdERG2 gene encoding sterol C-8 isomerase from V. dahliae and investigated its function in virulence by generating gene deletion mutants (ΔVdEGR2) and complemented mutants (C-ΔVdEGR2). Deletion of VdERG2 reduced ergosterol content. The conidial germination rate and conidial yield of ΔVdERG2 decreased signi cantly, and abnormal conidia were produced. In spite of VdERG2 did not affect the utilization of carbon sources by V. dahliae, but ΔVdERG2 observed a decrease in melanin production when cellulose and pectin were used as sole carbon sources, respectively. The ability of mutants ΔVdERG2 to produce microsclerotia and melanin decreased and the knockout of VdERG2 led to a signi cant decrease in the expression of microsclerotia and melanin-related genes Va M, Vayg1, VDH1, VdLAC, VdSCD and VT4HR. In addition, mutants ΔVdEGR2-1 and ΔVdEGR2-2 were very sensitive to congo red (CR), sodium dodecyl sulfate (SDS) and hydrogen peroxide (H 2 O 2 ) stresses, indicating that VdEGR2 was involved in cell wall and oxidative stress response. The absence of VdERG2 weakened the penetration ability of mycelium on cellophane and affected the growth of mycelium on cellophane. Although ΔVdERG2 could infect cotton, its pathogenicity was signi cantly impaired. These phenotypic defects in ΔVdERG2 could be complemented by reintroduction of a fulllength VdERG2 gene. In summary, as a single conservative secretory protein, VdERG2 played a crucial role in ergosterol biosynthesis, nutritional differentiation and virulence in V. dahliae.

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Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China

Liu

Chu

et al. 2023

J Basic Microbiol

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Fusarium wilt has occurred in the main Piper nigrum cultivation regions, which seriously affects the yield and quality of P. nigrum. To identify the pathogen of this disease, the diseased roots were collected from a demonstration base in Hainan Province. The pathogen was obtained by tissue isolation method and confirmed by pathogenicity test. Based on the morphological observation, sequence analyses of TEF1‐α nuclear gene, Fusarium solani was identified as the pathogen causing P. nigrum Fusarium wilt and induced symptoms on inoculated plants, including chlorosis, necrotic spots, wilt, drying, and root rot. The experiments for the antifungal activity showed that all the 11 fungicides selected in this study showed certain inhibitory effects on the colony growth of F. solani, where 2% kasugamycin AS, 45% prochloraz EW, 25 g·L−1 fludioxonil SC and 430 g·L−1 tebuconazole SC exhibited relative higher inhibitory effects with EC50 as 0.065, 0.205, 0.395, and 0.483 mg·L−1, respectively, and were selected to perform SEM analysis and test in seeds in vitro. The SEM analysis showed that kasugamycin, prochloraz, fludioxonil, and tebuconazole might have exerted their antifungal effect by damaging F. solani mycelia or microconidia. These preparations were applied as a seed coating of P. nigrum Reyin‐1. The kasugamycin treatment was most effective in reducing the harmful impact of F. solani on the seed germination. These results presented herein provide useful guidance for the effective control of P. nigrum Fusarium wilt.

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Identification and Functional Analysis of a Novel Hydrophobic Protein VdHP1 from Verticillium dahliae

Cited by 15 publications

References 82 publications

The Hydrophobin Gene Family Confers a Fitness Trade-off between Spore Dispersal and Host Colonization in Penicillium expansum

The Hydrophobin Gene Family Confers a Fitness Trade-off between Spore Dispersal and Host Colonization in Penicillium expansum

VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae

Identification and screening of fungicides against Piper nigrum basal Fusarium wilt disease in Hainan, China

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