Identification and Functional Analysis of Cytokine-Like Protein CLEC-47 in Caenorhabditis elegans

Pan, Wen; Huang, Xiaowen; Guo, Zeyuan; Nagarajan, Rekha; Mylonakis, Eleftherios

doi:10.1128/mbio.02579-21

Cited by 5 publications

(3 citation statements)

References 56 publications

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“…The C-type lectin domain containing protein CLEC-47 enhances the transcription of genes encoding for several antimicrobial and detoxifying proteins . The abundance of the protein is decreased according to our data set, implying a downregulation of the immune system in favor of coping with heat stress.…”

Section: Resultssupporting

confidence: 84%

“…The C-type lectin domain containing protein CLEC-47 enhances the transcription of genes encoding for several antimicrobial and detoxifying proteins. 51 The abundance of the protein is decreased according to our data set, implying a downregulation of the immune system in favor of coping with heat stress. In accordance with this, the abundance of a potential immune effector protein, namely the saposine-like protein SPP-10, a member of a family of antimicrobial peptides, 52 is here also found to be lower after heat treatment.…”

Section: Heat Shock Response In C Elegans On Protein Levelmentioning

confidence: 70%

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Digital Microfluidics Supported Microproteomics for Quantitative Proteome Analysis of Single Caenorhabditis elegans Nematodes

et al. 2022

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Miniaturization of sample preparation, including omissible manual sample handling steps, is key for reproducible nanoproteomics, as material is often restricted to only hundreds of cells or single model organisms. Here, we demonstrate a highly sensitive digital microfluidics (DMF)-based sample preparation workflow making use of single-pot solid-phase enhanced sample preparation (SP3) in combination with high-field asymmetric-waveform ion mobility spectrometry (FAIMS), and fast and sensitive ion trap detection on an Orbitrap tribrid MS system. Compared to a manual in-tube SP3-supported sample preparation, the numbers of identified peptides and proteins were markedly increased, while lower standard deviations between replicates were observed. We repeatedly identified up to 5000 proteins from single nematodes. Moreover, label-free quantification of protein changes in single Caenorhabditis elegans treated with a heat stimulus yielded 45 differentially abundant proteins when compared to the untreated control, highlighting the potential of this technology for low-input proteomics studies. LC-MS data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD033143.

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Section: Resultssupporting

confidence: 84%

Section: Heat Shock Response In C Elegans On Protein Levelmentioning

confidence: 70%

Digital Microfluidics Supported Microproteomics for Quantitative Proteome Analysis of Single Caenorhabditis elegans Nematodes

et al. 2022

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“…KEGG pathway annotation for day 1-6 DEG was enriched for the lysosome pathway including three cpr genes ( Cbr-cpr-1 , Cbr-cpr-3 , Cbr-cpr-4 ). In C. elegans , cpr class genes, together with caenacins cnc-1 and cnc-2 , have been shown to increase expression under influence of CLEC-47 overexpression, a signaling protein that activates the immune response (Pan et al, 2021). The upregulation of immune and stress response genes during the reproductive phase and subsequent downregulation is consistent with evolutionary pressure to maximize survival during the reproductive period and reproductive success.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide temporal gene expression reveals a post-reproductive shift in the nematodeC. briggsae

Berg,

Gupta

2024

Preprint

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C. briggsaeoffers a robust system for comparative investigations of genetic pathways that affect physiological processes. One key process, reproduction, significantly impacts longevity due to its high energetic cost, which limits resources for somatic maintenance. Long-lived mutants often exhibit reproductive impairments, and studies inC. eleganshave demonstrated that germline mutations and complete germline removal can promote longevity, underscoring the link between reproduction and aging. We are interested in identifying genes and biological processes affected during the reproductive and post-reproductive periods inC. briggsae. To achieve this, we conducted whole-genome transcriptome profiling on animals at various adult stages. analysis of differentially expressed (DE) genes revealed that the majority were downregulated during the reproductive period. Interestingly, this trend reversed post-reproduction, with three-quarters of the genes upregulated—a phenomenon we termed the ‘reproductive shift’. A similar analysis inC. elegansalso showed a downregulation bias during the reproductive period, but the reproductive shift was absent. Further examination ofC. briggsaeDE genes showed enrichment in processes related to the matrisome, muscle development and function during the reproductive period. Post-reproductive downregulated genes were enriched in DNA damage repair, stress response, and immune response. Additionally, terms related to fatty acid metabolism, catabolism, and transcriptional regulation exhibited complex patterns, with different biological processes being up or downregulated between the reproductive and post-reproductive stages. Overall, our transcriptomic data provides a valuable resource for cross-sectional comparative studies of reproductive and post-reproductive changes in nematodes. Additionally, the findings prompt similar studies in other animal models thereby advancing our understanding of genetic pathways affecting reproduction and aging.

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Paired C-type lectin receptors mediate specific recognition of divergent oomycete pathogens in C. elegans

Liu,

Grover,

Trusch

et al. 2024

Cell Reports

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Identification and Functional Analysis of Cytokine-Like Protein CLEC-47 in Caenorhabditis elegans

Abstract: A variety of effector proteins contribute to host defense in the nematode Caenorhabditis elegans . However, little is known about the exact function of these infection-related effectors beyond lytic enzymes and antimicrobial peptides and proteins.

Cited by 5 publications

References 56 publications

Digital Microfluidics Supported Microproteomics for Quantitative Proteome Analysis of Single Caenorhabditis elegans Nematodes

Digital Microfluidics Supported Microproteomics for Quantitative Proteome Analysis of Single Caenorhabditis elegans Nematodes

Genome-wide temporal gene expression reveals a post-reproductive shift in the nematodeC. briggsae

Paired C-type lectin receptors mediate specific recognition of divergent oomycete pathogens in C. elegans

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