Identification and functional analysis of transforming growth factor-β type III receptor (TβR3) from Scylla paramamosain: The first evidence of TβR3 involved in development and innate immunity in invertebrates

Li, Bo; Zhou, Yi; Gu, Wen Bin; Wang, Lan Zhi; Xu, Ya Ping; Cheng, Yuan; Chen, David D. Y.; Li, Bing Wu; Xiao, Yi; Wang, Dong; Miao, Shuying

doi:10.1016/j.fsi.2020.07.002

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…TGFβ signalling is an important signalling pathway potentially involved in sustaining ecdysteroid production and regulating the synthesis of 20E and juvenile hormone (Ishimaru et al 2016). In addition to promote moulting, both mTOR and TGFβ signalling pathways are also implicated in important growth and developmental processes; the former is an essential regulator of cell growth and proliferation (Zarogoulidis et al 2014; Hyun 2018) while the latter is involved in growth, development and innate immunity of invertebrates, including crustaceans (Li et al 2020).…”

Section: Ecdysone Signalling Pathway and Regulationmentioning

confidence: 99%

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Abdullah‐Zawawi

Afiqah‐Aleng

Ikhwanuddin

et al. 2021

Reviews in Aquaculture

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Ecdysone receptor (EcR) is the primary regulator of the ecdysteroid signalling pathway, a critical pathway that directly links to moulting. In addition, EcR also regulates growth, development, reproduction and regeneration in crustaceans. However, there remains a huge gap of knowledge between the detailed structure and functional role(s) of crustacean EcR compared to that of insects. Motif and phylogenetic analyses of publicly available crustacean EcR proteins revealed the evolutionary relationship of EcR in this subphylum and highlighted its conserved characteristics among crustaceans. The role of EcR in the regulation of essential physiological processes in crustaceans including moulting, chitin synthesis, general growth and development, gonadal and maturation, and limb regeneration was discussed based on the available literature. This essential moult-related nuclear receptor could serve as a useful molecular indicator of moulting and gonadal maturation, as a potential target for pesticide production against parasitic crustacean species in the aquaculture industry, as well as valuable bioindicators of environmental stressors.

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Section: Ecdysone Signalling Pathway and Regulationmentioning

confidence: 99%

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Abdullah‐Zawawi

Afiqah‐Aleng

Ikhwanuddin

et al. 2021

Reviews in Aquaculture

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“…The interaction between Smads and other signaling proteins mediates regulatory signals, controlling the expression of target genes, the translation of mRNA translation, and the regulation of cytoplasmic protein [ 8 , 9 , 10 , 11 ]. In recent years, the TGF-β superfamily has been found to play a critical role in the growth and development of shellfish [ 7 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Function of Bone Morphogenetic Protein 7 (BMP7) in the Pacific Abalone, Haliotis discus hannai

Huang

Zhou

Wang

et al. 2023

Genes

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Bone morphogenetic proteins (BMPs) play important roles in a lot of biological processes, such as bone development, cell proliferation, cell differentiation, growth, etc. However, the functions of abalone BMP genes are still unknown. This study aimed to better understand the characterization and biological function of BMP7 of Haliotis discus hannai (hdh-BMP7) via cloning and sequencing analysis. The coding sequence (CDS) length of hdh-BMP7 is 1251 bp, which encodes 416 amino acids including a signal peptide (1–28 aa), a transforming growth factor-β (TGF-β) propeptide (38–272 aa), and a mature TGF-β peptide (314–416 aa). The analysis of expression showed that hdh-BMP7 mRNA was widely expressed in all the examined tissues of H. discus hannai. Four SNPs were related to growth traits. The results of RNA interference (RNAi) showed that the mRNA expression levels of hdh-BMPR I, hdh-BMPR II, hdh-smad1, and hdh-MHC declined after hdh-BMP7 was silenced. After RNAi experiment for 30 days, the shell length, shell width, and total weight were found to be reduced in H. discus hannai (p < 0.05). The results of real-time quantitative reverse transcription PCR revealed that the hdh-BMP7 mRNA was lower in abalone of the S-DD-group than in the L-DD-group. Based on these data, we hypothesized that BMP7 gene has a positive role in the growth of H. discus hannai.

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“…In the presence of FSH, TGFβ1 is linked to the proliferation of mammalian follicular granulosa and increases the expression of LH receptors (LHR), the secretion of E 2 , and the production of follicular fluid [ 17 ]. In aquatic animals, TGFβ superfamily members are mainly involved in early embryonic development [ 18 ], immune regulation [ 19 , 20 ], and tissue regeneration [ 21 ]. The roles of members of the TGFβ superfamily in follicular development and atresia in fish are still unclear.…”

Section: Introductionmentioning

confidence: 99%

Upregulation of miR-33 Exacerbates Heat-Stress-Induced Apoptosis in Granulosa Cell and Follicular Atresia of Nile Tilapia (Oreochromis niloticus) by Targeting TGFβ1I1

Qiang

Tao

et al. 2022

Genes

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High temperature affects egg quality and increases follicular atresia in teleosts. The present study aimed to explore the regulated mechanism of ovary syndrome of Nile tilapia (Oreochromis niloticus) exposed to heat stress. To this end, we conducted histological and biochemical analyses and integrated miRNA-target gene analyses. The histochemical analyses confirmed that heat stress promoted the apoptosis of granulosa cell and therefore resulted in increased follicular atresia in the ovary. Heat stress led to the differential expression of multiple miRNAs (miR-27e, -27b-3p, -33, -34a -133a-5p, and -301b-5p). In a luciferase activity assay, miR-33 bound to the 3′-untranslated region (UTR) of the TGFβ1I1 (transforming growth factor-β1-induced transcript 1) gene and inhibited its expression. A TGFβ1I1 gene signal was detected in the granulosa cells of Nile tilapia by immunohistochemical analysis. Up-regulation of the miR-33 of tilapia at 6 d and 12 d exposed to heat (34.5 °C ± 0.5 °C) had significant down-regulation of the TGFβ1I1 expression of the gene and protein in tilapia ovaries. An miRNA-target gene integrated analysis revealed that miR-33 and TGFβ1I1 function in an apoptosis-related signal pathway. The signal transduction of the vascular endothelial growth factor (VEGF) family members VEGFA and its receptor (KDR) in the heat-stressed group decreased significantly compared with the control group. Transcript-levels of the Bax and Caspase-3 as apoptotic promotors were activated and Bcl-2 and Caspase-8 as apoptotic inhibitors were suppressed in the heat-stressed tilapia. These results suggest that heat stress increases the expression of miR-33, which targets TGFβ1I1 and inhibits its expression, resulting in decreased levels of follicle-stimulating hormone and 17β-estradiol and increased apoptosis by suppressing VEGF signaling, eventually inducing follicular atresia. In conclusion, our results show that the miR-33/TGFβ1I1 axis of Nile tilapia is involved in the follicular development of broodstock, and can suppress VEGF signaling to accelerate follicular atresia. Our findings demonstrate the suppressive role of miR-33 during oocyte development in Nile tilapia.

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Identification and functional analysis of transforming growth factor-β type III receptor (TβR3) from Scylla paramamosain: The first evidence of TβR3 involved in development and innate immunity in invertebrates

Cited by 6 publications

References 47 publications

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Recent development in ecdysone receptor of crustaceans: current knowledge and future applications in crustacean aquaculture

Molecular Characterization and Function of Bone Morphogenetic Protein 7 (BMP7) in the Pacific Abalone, Haliotis discus hannai

Upregulation of miR-33 Exacerbates Heat-Stress-Induced Apoptosis in Granulosa Cell and Follicular Atresia of Nile Tilapia (Oreochromis niloticus) by Targeting TGFβ1I1

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