Identification and functional characterization of the sulfate transporter gene GmSULTR1;2b in soybean

Ding, Yiqiong; Zhou, Xiaoqiong; Zuo, Li; Wang, Hui; Yu, Deyue

doi:10.1186/s12864-016-2705-3

Cited by 35 publications

(21 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further investigate potential roles for soybean NRAMP genes in nutrient homeostasis, GmNRAMPs were tested for alterations in expression in response to Fe or S deficiency. Two known marker genes, GmIRT (for low Fe) and GmSULTR1;2b (for low S) (Qin et al, 2012 ; Ding et al, 2016 ), were also applied to confirm the Fe or S deficiency in this study (Figures S2D,E ). As shown in Figures 6 , 9 GmNRAMP genes were significantly down-regulated by Fe deficiency.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

et al. 2017

View full text Add to dashboard Cite

The NRAMP (natural resistance-associated macrophage protein) family of genes has been widely characterized in organisms ranging from bacteria to yeast, plants, mice, and humans. This gene family plays vital roles in divalent metal ion transport across cellular membranes. As yet, comprehensive analysis of NRAMP family genes has not been reported for soybean. In this study, bioinformatics analysis was conducted to identify 13 soybean NRAMP genes, along with their gene structures, phylogenetic relationships, and transmembrane domains. Expression analysis suggests that GmNRAMP genes function in numerous tissues and development stages. Moreover, soybean NRAMP genes were differentially regulated by deficiencies of N, P, K, Fe, and S, along with toxicities of Fe, Cu, Cd, and Mn. These results indicate that GmNRAMP genes function in many nutrient stress pathways, and might be involved in crosstalk among nutrient stress pathways. Subcellular localization analysis in Arabidopsis protoplasts confirmed the tonoplast or plasma membrane localization of selected soybean NRMAP proteins. Protein-protein interaction analysis found that the networks of three GmNRAMP proteins which putatively interact with nodulin-like proteins, almost distinct from the network that is common to the other 10 soybean NRAMP proteins. Subsequent qRT-PCR results confirmed that these three GmNRMAP genes exhibited enhanced expression in soybean nodules, suggesting potential functions in the transport of Fe or other metal ions in soybean nodules. Overall, the systematic analysis of the GmNRAMP gene family reported herein provides valuable information for further studies on the biological roles of GmNRAMPs in divalent metal ion transport in various soybean tissues under numerous nutrient stresses and soybean-rhizobia symbiosis.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…20. Previously, Ding et al (2016) identified 28 putative sulfate transporter (SULTR) genes in the soybean genome, including a member of the SULTR3 subfamily near the locus on chr. 20.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Genomic Loci Associated with Soybean Shoot Tissue Macro and Micronutrient Concentrations

Dhanapal

Ray²,

Smith³

et al. 2018

The Plant Genome

View full text Add to dashboard Cite

The mineral composition of crop shoot tissues is important for yield formation and nutrient remobilization to seeds. The natural diversity that exists within crop species can be used to investigate mechanisms that define plant mineral composition and to identify important genomic loci for these processes. The objective of this study was to determine shoot mineral nutrient concentrations in genetically diverse soybean [ (L.) Merr.] genotypes and to identify genomic regions associated with concentrations of different nutrients in shoot tissue. The genotypes were grown at two locations in 2 yr and characterized for macronutrient (Ca, Mg, P, K, and S) and micronutrient (B, Cu, Fe, Mn, and Zn) concentrations in shoot tissues. Genome-wide association studies were conducted with 31,748 single nucleotide polymorphisms (SNPs) via a unified mixed model to identify SNPs associated with macro- and micronutrient concentrations. The number of putative loci identified for the macronutrients ranged from 11 for Ca to 20 for K. For the micronutrients, the number ranged from 10 for Mn to 24 for Fe. In addition to colocated loci for multiple nutrients, 22 individual SNPs were associated with more than one nutrient such that 11 different nutrient combinations were encompassed by these SNPs. Ultimately, the putative loci identified in this study will need to be confirmed and are expected to aid in the identification of new sources of variation for use in soybean breeding programs as well as for mechanistic studies aimed at understanding the regulation of mineral nutrient uptake, translocation, and shoot tissue concentrations.

show abstract

“…Among those dietary foods that have pharmacologically active metabolites, soybean ( Glycine max L.), which is commonly consumed in traditional Asian food, is a major source of oil and protein in the human and animal diet [4]. It is one of the natural substitutes for the treatment of a variety of hormone-dependent and independent disorders in humans [5].…”

Section: Introductionmentioning

confidence: 99%

Equol, a Dietary Daidzein Gut Metabolite Attenuates Microglial Activation and Potentiates Neuroprotection In Vitro

et al. 2017

View full text Add to dashboard Cite

Estrogen deficiency has been well characterized in inflammatory disorders including neuroinflammation. Daidzein, a dietary alternative phytoestrogen found in soy (Glycine max) as primary isoflavones, possess anti-inflammatory activity, but the effect of its active metabolite Equol (7-hydroxy-3-(4′-hydroxyphenyl)-chroman) has not been well established. In this study, we investigated the anti-neuroinflammatory and neuroprotective effect of Equol in vitro. To evaluate the potential effects of Equol, three major types of central nervous system (CNS) cells, including microglia (BV-2), astrocytes (C6), and neurons (N2a), were used. Effects of Equol on the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), Mitogen activated protein kinase (MAPK) signaling proteins, and apoptosis-related proteins were measured by western blot analysis. Equol inhibited the lipopolysaccharide (LPS)-induced TLR4 activation, MAPK activation, NF-kB-mediated transcription of inflammatory mediators, production of nitric oxide (NO), release of prostaglandin E2 (PGE-2), secretion of tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), in Lipopolysaccharide (LPS)-activated murine microglia cells. Additionally, Equol protects neurons from neuroinflammatory injury mediated by LPS-activated microglia through downregulation of neuronal apoptosis, increased neurite outgrowth in N2a cell and neurotrophins like nerve growth factor (NGF) production through astrocytes further supporting its neuroprotective potential. These findings provide novel insight into the anti-neuroinflammatory effects of Equol on microglial cells, which may have clinical significance in cases of neurodegeneration.

show abstract

Identification and functional characterization of the sulfate transporter gene GmSULTR1;2b in soybean

Cited by 35 publications

References 70 publications

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

Identification of Novel Genomic Loci Associated with Soybean Shoot Tissue Macro and Micronutrient Concentrations

Equol, a Dietary Daidzein Gut Metabolite Attenuates Microglial Activation and Potentiates Neuroprotection In Vitro

Contact Info

Product

Resources

About