Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Zhan, Hao; Du, Li-Lin; Dewer, Youssef; Niu, Changying; Li, Fengqi; Luo, Chen

doi:10.1016/j.heliyon.2021.e06427

Cited by 6 publications

(4 citation statements)

References 46 publications

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“…From these pieces of evidence, future projects may investigate the functional properties of DsuzOR19A2 expressed in at1a OSNs. To these attempts, D. suzukii cuticular hydrocarbons (Snellings et al, 2018;Wang et al, 2020), headspace collections (Kwadha et al, 2021;Cattaneo et al, 2022), or candidate sex pheromones, such as (Z)-7-tricosene (Zhan et al, 2021) or (Z)-9-tricosene (Lima et al, 2023), may be tested on this subunit in search of its main ligands.…”

Section: Discussionmentioning

confidence: 99%

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Pettersson,

Cattaneo

2024

Front. Mol. Biosci.

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In insects, antennal ionotropic receptors (IRs) and odorant receptors (ORs) are among the main sensors of olfactory cues. To functionally characterize the subunits from these receptors, the use of ab3A neurons from transgenic Drosophila melanogaster represented one of the most powerful tools, allowing the identification of ligands (deorphanization) and decrypting their pharmacological properties. However, further investigation is needed to shed light on possible metabotropic functionalities behind insect olfactory receptors and test potentials from the up-to-now-used empty neuronal systems to express subunits belonging to variegate receptor classes. In this project, we adopted the most updated system of Drosophila ab3A empty neurons to test various olfactory receptors, ranging from human ORs working as metabotropic G-protein coupled receptors to insect ionotropic IRs and ORs. Testing transgenic Drosophila expressing human ORs into ab3A neurons by single sensillum recording did not result in an OR response to ligands, but it rather re-established neuronal spiking from the empty neurons. When transgenic D. melanogaster expressed ionotropic IRs and ORs, both heterologous and cis-expressed IRs were non-functional, but the Drosophila suzukii OR19A1 subunit responded to a wide asset of ligands, distinguishing phasic or tonic compound-dependent effects. Despite the use of Drosophila ab3A neurons to test the activation of some metabotropic and ionotropic receptor subunits resulted non-functional, this study deorphanized a key OR of D. suzukii demonstrating its binding to alcohols, ketones, terpenes, and esters.

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Section: Discussionmentioning

confidence: 99%

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Pettersson,

Cattaneo

2024

Front. Mol. Biosci.

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“…The docking analysis of the interaction between Hh Or85b and the aggregation pheromone was not carried out, because we could not detect an appropriate template with reasonable homology. In insects, the recognition of pheromones often involves multiple odorant-binding proteins and chemoreceptors [ 49 , 51 , 52 , 53 ]. Therefore, in addition to the Hh CSP5 identified in this study, there may be other OBPs or CSPs responsible for the recognition and transport of aggregation pheromones in H. halys .…”

Section: Discussionmentioning

confidence: 99%

Exploration of Candidate Genes Involved in the Biosynthesis, Regulation and Recognition of the Male-Produced Aggregation Pheromone of Halyomorpha halys

Zhang

Dewer

et al. 2023

Insects

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The aggregation pheromone of the brown marmorated stink bug, Halyomorpha halys (Stål), is produced by adult males, and plays an important role in the behavioral regulation of H. halys. However, information on the molecular mechanisms underlying this pheromone’s biosynthesis is limited. In this study, HhTPS1, a key candidate synthase gene in the aggregation pheromone biosynthesis pathway of H. halys, was identified. Then, through weighted gene co-expression network analysis, the candidate P450 enzyme genes in the biosynthetic downstream of this pheromone and the related candidate transcription factor in this pathway were also identified. In addition, two olfactory-related genes, HhCSP5 and HhOr85b, involved in the recognition of the aggregation pheromone of H. halys, were detected. We further identified the key amino acid sites of HhTPS1 and HhCSP5 that interact with substrates by using molecular docking analysis. This study provides basic information for further investigations into the biosynthesis pathways and recognition mechanisms of aggregation pheromones in H. halys. It also provides key candidate genes for bioengineering bioactive aggregation pheromones necessary for the development of technologies for the monitoring and control of H. halys.

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“…In this study, MusiOBP1 and MusiCSP1 had good binding affinities with 1-NPN. Similar to research on DsOBP69a and DsOBP76a ( Zhan et al, 2021 ), after adding 1-NPN and the protein solution, we added 2–16 μmol/L odor molecules to determine the dissociation constants. Although at 16 μmol/L, the compound with the strongest binding affinity to proteins can only reduce the fluorescence intensity to < 60%, IC50 and Ki calculations can reflect the binding affinity of proteins with each odor molecule.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of megalurothrips usitatus (Bagnall) identifies olfactory genes with ligands binding characteristics of MusiOBP1 and MusiCSP1

Chen

Wang

et al. 2022

Front. Physiol.

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The olfactory system is an important component of insect behavior and is vital for survival and reproduction. However, the genomic characterization and molecular basis of the olfactory response of Megalurothrips usitatus remain relatively unknown. RNA sequencing-built developmental transcriptomes of nymphs, pupae, and adult M. usitatus were examined in order to establish the sequence-based background of M. usitatus olfactory responses. A total of 56,669 unigenes were annotated using GO, NR, Pfam, eggNOG, SwissProt, and KEGG. The number of differentially expressed genes between pupae and nymphs, males and nymphs, and females and nymphs were 10,498, 9,235, and 10,964, respectively. One odorant-binding protein (MusiOBP1) and one chemosensory protein (MusiCSP1) were selected from the transcriptome, and their full-length sequences were obtained using RACE PCR. The relative expression of MusiOBP1 was the highest in primordial females, whereas the relative expression of MusiCSP1 was the highest in primordial pupae. The strongest binding ability to the odor-binding protein MusiOBP1 was observed for β-citronellol. 3-Hydroxy-2-methyl-4-pyrone showed the strongest binding affinity to MusiCSP1. Our analysis suggests that MusiOBP1 and MusiCSP1 may play significant roles in mediating M. usitatus host recognition. This research will improve our knowledge of odorant-binding proteins and chemosensory proteins, which will in turn improve our understanding of insect olfactory systems.

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Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Cited by 6 publications

References 46 publications

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Heterologous investigation of metabotropic and ionotropic odorant receptors in ab3A neurons of Drosophila melanogaster

Exploration of Candidate Genes Involved in the Biosynthesis, Regulation and Recognition of the Male-Produced Aggregation Pheromone of Halyomorpha halys

Transcriptome analysis of megalurothrips usitatus (Bagnall) identifies olfactory genes with ligands binding characteristics of MusiOBP1 and MusiCSP1

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