Identification and functional characterization of the siRNA pathway in Taenia crassiceps by silencing Enolase A

Guerrero-Hernández, Julio; Bobes, Raúl J.; García-Varela, Martìn; Castellanos-González, Alejandro; Laclette, Juan Pedro

doi:10.1016/j.actatropica.2021.106197

Cited by 2 publications

(2 citation statements)

References 66 publications

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“…Survival of this strain in natural conditions appears to be impractical. The role of 533 specific genes in the formation of the scolex could be approached through gene 534 silencing experiments on WFU cysts ( Guerrero-Hernández et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

Bobes

Estrada

Ríos-Valencia

et al. 2022

Front. Cell. Infect. Microbiol.

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Human cysticercosis by Taenia solium is the major cause of neurological illness in countries of Africa, Southeast Asia, and the Americas. Publication of four cestode genomes (T. solium, Echinococcus multilocularis, E. granulosus and Hymenolepis microstoma) in the last decade, marked the advent of novel approaches on the study of the host-parasite molecular crosstalk for cestode parasites of importance for human and animal health. Taenia crassiceps is another cestode parasite, closely related to T. solium, which has been used in numerous studies as an animal model for human cysticercosis. Therefore, characterization of the T. crassiceps genome will also contribute to the understanding of the human infection. Here, we report the genome of T. crassiceps WFU strain, reconstructed to a noncontiguous finished resolution and performed a genomic and differential expression comparison analysis against ORF strain. Both strain genomes were sequenced using Oxford Nanopore (MinION) and Illumina technologies, achieving high quality assemblies of about 107 Mb for both strains. Dotplot comparison between WFU and ORF demonstrated that both genomes were extremely similar. Additionally, karyotyping results for both strains failed to demonstrate a difference in chromosome composition. Therefore, our results strongly support the concept that the absence of scolex in the ORF strain of T. crassiceps was not the result of a chromosomal loss as proposed elsewhere. Instead, it appears to be the result of subtle and extensive differences in the regulation of gene expression. Analysis of variants between the two strains identified 2,487 sites with changes distributed in 31 of 65 scaffolds. The differential expression analysis revealed that genes related to development and morphogenesis in the ORF strain might be involved in the lack of scolex formation.

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Section: Discussionmentioning

confidence: 99%

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

Bobes

Estrada

Ríos-Valencia

et al. 2022

Front. Cell. Infect. Microbiol.

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“…RNAi was first identified in the nematode worm C. elegans and has since been widely applied to the molecular characterization and functional analysis of parasites of medical-veterinary importance [30]. Examples of its application include the characterization of enolase A in Taenia crassiceps [31], and a matrix metalloprotease 12A in Haemonchus contortus [32]. In this study, we utilized RNAi, an excellent approach for expediting research in cell and molecular biology, to explore the function of TsDNase II-7.…”

Section: Discussionmentioning

confidence: 99%

In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells

Wang¹,

Jin²,

Li³

et al. 2023

PLoS Negl Trop Dis

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Trichinella spiralis (T. spiralis) adult-specific deoxyribonuclease II-7 (TsDNase II-7), a member of the DNase II-like nuclease family with no DNase II activity, was identified in the excretory–secretory (ES) products of adult worms (AWs). However, its biological functions are still unclear. Our previous study revealed that TsDNase II-7 is located around the infection site in the intestinal tissue, speculating that it was involved in the T. spiralis invasion of host intestinal epithelial cells (IECs). This study aimed to use RNA interference to verify our speculation that TsDNase II-7 in 3-day old adult T. spiralis (Ad3) plays a role in intestinal invasion. TsDNase II-7-specific small interfering RNAs (siRNAs) were delivered into muscle larvae (MLs) to knockdown TsDNase II-7 expression by electroporation. Twenty-four hours later, the MLs transfected with 2 μM siRNA-841 exhibited decreased in TsDNase II-7 transcription and expression as compared to the control MLs. The knockdown of TsDNase II-7 expression did not affect ML viability, and the low expression of TsDNase II-7 still maintained in Ad3 recovered from TsDNase II-7-RNAi-ML infected mice, resulting in a weakened ability of Ad3 to invade intestinal epithelial cells (IECs). These results indicated that knockdown of TsDNase II-7 gene expression via RNA interference (RNAi) suppressed adult worm invasion and confirmed that TsDNase II-7 plays a crucial role during the intestinal phase of T. spiralis infections, which provided new candidate for vaccine development of T. spiralis.

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Identification and functional characterization of the siRNA pathway in Taenia crassiceps by silencing Enolase A

Cited by 2 publications

References 66 publications

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

The Genomes of Two Strains of Taenia crassiceps the Animal Model for the Study of Human Cysticercosis

In vitro knockdown of TsDNase II-7 suppresses Trichinella spiralis invasion into the host’s intestinal epithelial cells

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