Identification and functional characterization of Trypanosoma brucei peroxin 16

Kalel, Vishal C.; Schliebs, Wolfgang; Erdmann, Ralf

doi:10.1016/j.bbamcr.2015.05.024

Cited by 28 publications

(32 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, a Pex16 homolog was recently identified in T. brucei and silencing of this glycosome protein results in glycosome abnormalities with accumulation of structures in the anterior part of the cells where ER exit sites are located (Kalel et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…The mechanisms of glycosome biogenesis are not fully understood, although comparison of components required for peroxisome biosynthesis has been useful in resolving potential contributing proteins (Galland and Michels ; Kalel et al. ). For example, homologs of 10 of the more than 58 described eukaryotic peroxin (Pex) proteins have been identified in T. brucei .…”

mentioning

confidence: 99%

See 1 more Smart Citation

Localization of a Trypanosome Peroxin to the Endoplasmic Reticulum

Bauer

McQueeney

Patel

et al. 2016

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Trypanosoma brucei is the causative agent of diseases that affect 30,000–50,000 people annually. Trypanosoma brucei harbors unique organelles named glycosomes that are essential to parasite survival, which requires growth under fluctuating environmental conditions. The mechanisms that govern the biogenesis of these organelles are poorly understood. Glycosomes are evolutionarily related to peroxisomes, which can proliferate de novo from the endoplasmic reticulum or through the growth and division of existing organelles depending on the organism and environmental conditions. The effect of environment on glycosome biogenesis is unknown. Here, we demonstrate that the glycosome membrane protein, TbPex13.1, is localized to glycosomes when cells are cultured under high glucose conditions and to the endoplasmic reticulum in low glucose conditions. This localization in low glucose was dependent on the presence of a C‐terminal tripeptide sequence. Our findings suggest that glycosome biogenesis is influenced by extracellular glucose levels and adds to the growing body of evidence that de novo glycosome biogenesis occurs in trypanosomes. Because the movement of peroxisomal membrane proteins is a hallmark of ER‐dependent peroxisome biogenesis, TbPex13.1 may be a useful marker for the study such processes in trypanosomes.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Localization of a Trypanosome Peroxin to the Endoplasmic Reticulum

Bauer

McQueeney

Patel

et al. 2016

J Eukaryotic Microbiology

View full text Add to dashboard Cite

show abstract

“…A considerable number of T. brucei homologs of mammalian and yeast peroxins involved in PMP insertion and matrix protein import have been identified and characterized, and shown to be involved in glycosome biogenesis (Kalel et al, 2015(Kalel et al, , 2019Banerjee et al, 2019;and reviewed in Galland and Michels, 2010;Gualdrón-López et al, 2013). Orthologous genes have been detected in the genome of T. cruzi and several of the proteins in its glycosomal proteome (Acosta et al, 2019).…”

Section: Biogenesis Of Glycosomesmentioning

confidence: 99%

Structure, Properties, and Function of Glycosomes in Trypanosoma cruzi

Quiñones

Acosta

Gonçalves

et al. 2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Glycosomes are peroxisome-related organelles that have been identified in kinetoplastids and diplonemids. The hallmark of glycosomes is their harboring of the majority of the glycolytic enzymes. Our biochemical studies and proteome analysis of Trypanosoma cruzi glycosomes have located, in addition to enzymes of the glycolytic pathway, enzymes of several other metabolic processes in the organelles. These analyses revealed many aspects in common with glycosomes from other trypanosomatids as well as features that seem specific for T. cruzi. Their enzyme content indicates that T. cruzi glycosomes are multifunctional organelles, involved in both several catabolic processes such as glycolysis and anabolic ones. Specifically discussed in this minireview are the cross-talk between glycosomal metabolism and metabolic processes occurring in other cell compartments, and the importance of metabolite translocation systems in the glycosomal membrane to enable the coordination between the spatially separated processes. Possible mechanisms for metabolite translocation across the membrane are suggested by proteins identified in the organelle's membrane-homologs of the ABC and MCF transporter families-and the presence of channels as inferred previously from the detection of channel-forming proteins in glycosomal membrane preparations from the related parasite T. brucei. Together, these data provide insight in the way in which different parts of T. cruzi metabolism, although uniquely distributed over different compartments, are integrated and regulated. Moreover, this information reveals opportunities for the development of drugs against Chagas disease caused by these parasites and for which currently no adequate treatment is available.

show abstract

“…The biogenesis of peroxisomes and glycosomes can involve new organelles that arise from pre-existing peroxisomal/glycosomal structures or de novo synthesis from the endoplasmic reticulum. However, in eukaryotes subject to environmental change, peroxisome composition can be modulated to allow metabolic adaptation, and this can be achieved by de novo loading from the ER (27) as well as the growth and division of existing glycosomes. The dynamics of environmental adaptation in procyclic form parasites have been analysed both during differentiation and upon exposure of procyclic forms to low and high glucose concentrations.…”

Section: Discussionmentioning

confidence: 99%

Positional dynamics and glycosomal recruitment of developmental regulators during trypanosome differentiation

Szöőr

Simon

Rojas

et al. 2019

Preprint

View full text Add to dashboard Cite

27Glycosomes are peroxisome-related organelles that compartmentalise the glycolytic enzymes 28 in kinetoplastid parasites. These organelles are developmentally regulated in their number 29 and composition, allowing metabolic adaptation to the parasite's needs in the blood of 30 mammalian hosts or within their arthropod vector. A protein phosphatase cascade regulates 31 differentiation between parasite developmental forms, comprising a tyrosine phosphatase, 32TbPTP1, that dephosphorylates and inhibits a serine threonine phosphatase TbPIP39 that 33 promotes differentiation. When TbPTP1 is inactivated, TbPIP39 is activated and during 34 differentiation becomes located in glycosomes. Here we have tracked TbPIP39 recruitment to 35 glycosomes during differentiation from bloodstream stumpy forms to procyclic forms. 36Detailed microscopy and live cell imaging during the synchronous transition between life 37 cycle stages revealed that in stumpy forms, TbPIP39 is located at a periflagellar pocket site 38 closely associated with TbVAP, that defines the flagellar pocket endoplasmic reticulum. 39 TbPTP1 is also located at the same site in stumpy forms, as is REG9.1, a regulator of 40 stumpy-enriched mRNAs. This site provides a molecular node for the interaction between 41 TbPTP1 and TbPIP39. Within 30 minutes of the initiation of differentiation TbPIP39 42 relocates to glycosomes whereas TbPTP1 disperses to the cytosol. Overall, the study 43 identifies a 'stumpy regulatory nexus' (STuRN) that co-ordinates the molecular components 44 of life cycle signalling and glycosomal development during transmission of Trypanosoma 45 brucei. 46 47 48 49 3 50 Importance 51 52 African trypanosomes are parasites of sub-Saharan Africa responsible for both human and 53 animal disease. The parasites are transmitted by tsetse flies and completion of their life cycle 54 involves progression through several development steps. The initiation of differentiation 55between blood and tsetse forms is signalled by a phosphatase cascade, ultimately trafficked 56 into peroxisome-related organelles called glycosomes that are unique to this group of 57 organisms. Glycosomes undergo substantial remodelling of their composition and function 58 during the differentiation step but how this is regulated is not understood. Here we identify a 59 cytological site where the signalling molecules controlling differentiation converge before the 60 dispersal of one of them into glycosomes. This coincides with a specialised ER site that may 61 contribute to glycosome developmental biogenesis or regeneration. In combination, the study 62 provides the first insight into the spatial co-ordination of signalling pathway components in 63 trypanosomes as they undergo cell-type differentiation. 64 65 66 4 67 Introduction 68The dynamic regulation of organelle biogenesis or composition often involves intimate 69 contact between the endoplasmic reticulum and the membrane of the target organelle (1). 70This enables the maturation and modification of organellar protein content, influ...

show abstract

Identification and functional characterization of Trypanosoma brucei peroxin 16

Cited by 28 publications

References 64 publications

Localization of a Trypanosome Peroxin to the Endoplasmic Reticulum

Localization of a Trypanosome Peroxin to the Endoplasmic Reticulum

Structure, Properties, and Function of Glycosomes in Trypanosoma cruzi

Positional dynamics and glycosomal recruitment of developmental regulators during trypanosome differentiation

Contact Info

Product

Resources

About