Identification and Functional Characterization of the Soybean GmaPPO12 Promoter Conferring Phytophthora sojae Induced Expression

Chai, Chun-Yue; Lin, Yanling; Shen, Danyu; Wu, Yuanyi; Li, Hongjuan; Dou, Daolong

doi:10.1371/journal.pone.0067670

Cited by 32 publications

(28 citation statements)

References 71 publications

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“…LUC ( Figure 6A) and GUS ( Figure 6B) activities also support this finding of OsPPO promoter induction with higher activity by ABA at 350 µM, while LUC ( Figure 7A) and GUS ( Figure 7B) activities remained unresponsive to MeJ treatments. Similar results were found in Glycine max PPO gene promoter, which showed 2.5-fold upregulation by ABA but not by MeJ (Chai et al, 2013). Moreover, banana PPO gene also remained unresponsive to MeJ (Gooding et al, 2001).…”

Section: Induction Of Osppo Transcript In Response To Aba and Mej Tresupporting

confidence: 82%

“…In some plant species PPO activity is strongly induced by insect attacks (Ruuhola et al, 2008). Using modified PPO expression in transgenic plants, a defensive role was established against insects (Barbehenn et al, 2007;Mahanil et al, 2008;Bhonwong et al, 2009) and pest infestations (Wang and Constabel, 2004;Richter et al, 2012;Chai et al, 2013). As anticipated, the downregulation or silencing of PPOs resulted in enhanced susceptibility to pathogens in transgenic tomato and potato lines (Thipyapong et al, 2004;Richter et al, 2012).…”

Section: Introductionmentioning

confidence: 83%

“…Chai et al (2013) reported the presence of MYB and MYC cis-regulatory elements in Glycin max PPO promoter, which was found to be ABA and Phytophtora inducible. Structural analysis of OsPPO promoter confirms the key motifs (DPB, MYB, MYC, WRKY, and W-boxes) for ABA and wound signaling.…”

Section: Signal Scan (Place) Shows Cis-regulatory Elements For Aba Anmentioning

confidence: 99%

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Characterization of rice polyphenol oxidase promoter in transgenic Arabidopsis thaliana

Akhtar¹,

Ejaz²,

Koiwa³

et al. 2017

Turk J Bot

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IntroductionPlants act in response to pathogens attack through activation of many mechanisms, ultimately resulting in reduction of growth and control of pathogens. Polyphenol oxidases (PPOs) are copper metalloproteins that function in plant defense but also cause significant postharvest agricultural losses (Aniszewski et al., 2008). PPO enzymes are inert in thaylakoid and become active upon release from the thylakoid by disruption such as wounding, senescence, and attack by insect pests or pathogens. PPOs catalyze oxygen-dependent oxidation of phenols to quinones (brown phenomenon). These black and brown quinone adducts formed by PPO enzymatic activity are renowned and the reason for interest in the postharvest physiology of many vegetable and fruit crops (Mayer and Harel, 1979;Friedman, 1997). These quinones are highly reactive intermediates that undergo secondary reactions and ultimately bring about oxidative browning that accompanies plant senescence, wounding, and responses to pathogens (Thipyapong et al., 2004). Therefore, PPO gene activity ultimately enables quinones and reactive oxygen species to cope with stresses. Due to prominent wound and pathogen inducibilities, PPOs are significant in biological studies (Thipyapong et al., 1997).PPO expression in transgenic plants provides a unique system to evaluate the involvement of PPO in plant disease resistance. The role of PPO in 'induced plant defense' has been validated in transgenic plants. PPO overexpressing lines showed increased resistance to bacterial pathogens (Li and Steffens, 2002;Richter et al., 2012). In some plant species PPO activity is strongly induced by insect attacks (Ruuhola et al., 2008). Using modified PPO expression in transgenic plants, a defensive role was established against insects (

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Section: Induction Of Osppo Transcript In Response To Aba and Mej Tresupporting

confidence: 82%

Section: Introductionmentioning

confidence: 83%

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Characterization of rice polyphenol oxidase promoter in transgenic Arabidopsis thaliana

Akhtar¹,

Ejaz²,

Koiwa³

et al. 2017

Turk J Bot

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“…Histochemical b-glucuronidase (GUS) assays and quantitative RT-PCR techniques were used following methods described previously (Jefferson, 1989;Chai et al, 2013). Nicotian tabacum elongation factor 1-a mRNA (AF120093.1) was used as the internal control.…”

Section: Promoter Activity Analysismentioning

confidence: 99%

Small interfering RNAs from bidirectional transcripts of GhMML3_A12 regulate cotton fiber development

et al. 2016

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SummaryNatural antisense transcripts (NATs) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved in gene regulation in plants. In this research, we investigated the function of small RNA derived from a NAT in fiber cell development.Using a map-based cloning strategy for the first time in tetraploid cotton, we cloned a naked seed mutant gene (N 1 ) encoding a MYBMIXTA-like transcription factor 3 (MML3)/GhMYB25-like in chromosome A12, GhMML3_A12, that is associated with fuzz fiber development.The extremely low expression of GhMML3_A12 in N 1 is associated with NAT production, driven by its 3 0 antisense promoter, as indicated by the promoter-driven histochemical staining assay. In addition, small RNA deep sequencing analysis suggested that the bidirectional transcriptions of GhMML3_A12 form double-stranded RNAs and generate 21-22 nt small RNAs. Therefore, in a fiber-specific manner, small RNA derived from the GhMML3_A12 locus can mediate GhMML3_A12 mRNA self-cleavage and result in the production of naked seeds followed by lint fiber inhibition in N 1 plants. The present research reports the first observation of gene-mediated NATs and siRNA directly controlling fiber development in cotton.

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“…For instance, tomato has seven PPOs, potato has six PPOs and soybean has eleven PPOs (Newman et al, 2011;Urbany et al, 2011;Tran et al, 2012;Chai et al, 2013). These features have complicated the search of polymorphisms between PPOs because amplification of a single isoform is difficult.…”

Section: Synteny and Orthologous Candidate Genes With Other Mapsmentioning

confidence: 99%

Genetics and Genomics of Cultivated Eggplants and Wild Relatives

Gramazio¹

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"Nothing in life is to be feared, it is only to be understood. Now is the time to understand more, so that we may fear less." Maria Salomea Skłodowska-Curie AgradecimientosAunque parezcan mucho menos, ya siete son los años que llevo en el COMAV. Los años pasan volando cuando el ambiente de trabajo es estimulante, constructivo, apasionante, gratificante, motivador, intrigante, creativo, integrador, cooperativo, productivo, positivo, divertido…, familiar.Todo empezó aquella tarde del 10 de noviembre del 2010, cuando Lola Raigón, a quien va mi eterna gratitud, me presentó la persona que iba a cambiar mi vida, Jaime Prohens.Jaime, la admiración, el respeto y la gratitud que siento por ti no se pueden expresar en unas pocas frases. Eres la persona que más me ha enseñado, motivado y animado en estos años. Has conseguido sacar de mí unas cualidades que ni siquiera había imaginado tener. Tu inteligencia, sabiduría, humildad, paciencia, hacen de ti un verdadero líder. Gracias por estar pendiente en cada momento, espero colaborar contigo durante muchos años más. Eres un ejemplo a seguir y fuente de inspiración y guía para mi y muchas personas.Mi formación doctoral no sería completa si no hubiera sido por tí, Santi. Intelligente, simpatíco, caótico, siempre de buen humor y "runner".Gracias por todo el conocimiento, las buenas ideas, las clases de Génomica, las risas y los videos "frikis" que has compartido conmigo. Gracias por alegrar nuestros días.Mariola, aunque burocráticamente no has podido ser mi co-directora, te agradezco el apoyo, motivación y alegría de estos años. Aunque ahora pasamos todo el día en los despachos, echo de menos los primeros años de laboratorio, cuando pasábamos horas y horas hablando de lo que fuera. Junto a Jaime y Santi, eres un pilar de "Los Berenjen@s".Gracias a mi "hermano mayor" Javi, por los muchos momentos de amistad pasados juntos, por tu inteligencia, por compartir tus experiencias, por tu buen gusto musical, tu escepticismo, por ser un gran amigo."Los Berenjen@s" se han ido multiplicando y muchas son las entradas que hemos recolectado en estos años alrededor del mundo. Me hubiera gustado escribir algo sobre cada uno de vosotros, pero necesitaría otro capítulo de tesis, así que os agradeceré de forma conjuntas.Gracias Abou, Adriana, Agustín, Alba E., Alba L., Alessio, Andrea, Arantxa, Arunika, Bianca, Brice, Camil, Carolina, Celia, Celine, Cristina, Dani, David, Dionís, Dora, Edgar, Elena C., Elena R., Elisa, Francesco, Giulio, Gloria, Ioana, Irene, Isa, Juan, Juan Pablo, Judith, Lahiru, Lara, Laura, Loles, Maria, Marieta, Michael, Muditha, Nacho, Nadia, Nirasha, Noelia, Orsi, Pelin, Prashant, Ramses, Ranil, Rory, Silia, Sorin, Trong, por haber aportado vuestro granito de arena para la realización de esta tesis y de colaboraciones varias. Todos, los que todavía estáis por aquí y los que se han ido, habéis dejado una huella en el laboratorio y en mí (Pido disculpa si se me ha olvidado alguien, sois muchos los que se han pasado por el labo).Gracias también a todos mis compañeros del COMAV,...

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Identification and Functional Characterization of the Soybean GmaPPO12 Promoter Conferring Phytophthora sojae Induced Expression

Cited by 32 publications

References 71 publications

Characterization of rice polyphenol oxidase promoter in transgenic Arabidopsis thaliana

Characterization of rice polyphenol oxidase promoter in transgenic Arabidopsis thaliana

Small interfering RNAs from bidirectional transcripts of GhMML3_A12 regulate cotton fiber development

Genetics and Genomics of Cultivated Eggplants and Wild Relatives

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