Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis

Liu, Jianbai; Wu, Han; Yi, Jiequn; Jiang, Dingxin; Zhang, Guren

doi:10.1371/journal.pone.0217493

Cited by 11 publications

(14 citation statements)

References 71 publications

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“…This approach was chosen because manipulation of primary metabolism in plants, including sugar complementation, results in pleiotropic effects [ 78 , 79 ]. Sugar perception in insects is highly conserved and mediated by Gr43a -like genes [ 49 , 50 , 53 , 54 , 80 – 83 ]. BLAST search of publicly available nucleotide sequences revealed a single putative Gr43a gene in the western corn rootworm, here named DvvGr43a ( Fig 3A ).…”

Section: Resultsmentioning

confidence: 99%

“…The full gene sequence can be retrieved from the National Center for Biotechnology Information (NCBI) data bank using the following accession number: XM_028279548.1. Then, we reconstructed evolutionary relationships between the identified DvvGr43a gene and several other putative Gr43a genes that have been functionally characterized as glucose, fructose, and/or sucrose receptors [ 49 , 50 , 53 , 54 , 80 – 83 ]. The evolutionary relationships were inferred using the Maximum Likelihood method based on the Le Gascuel 2008 model in MEGA7 using protein sequences [ 113 , 114 ].…”

Section: Methodsmentioning

confidence: 99%

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The plant metabolome guides fitness-relevant foraging decisions of a specialist herbivore

et al. 2021

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Plants produce complex mixtures of primary and secondary metabolites. Herbivores use these metabolites as behavioral cues to increase their fitness. However, how herbivores combine and integrate different metabolite classes into fitness-relevant foraging decisions in planta is poorly understood. We developed a molecular manipulative approach to modulate the availability of sugars and benzoxazinoid secondary metabolites as foraging cues for a specialist maize herbivore, the western corn rootworm. By disrupting sugar perception in the western corn rootworm and benzoxazinoid production in maize, we show that sugars and benzoxazinoids act as distinct and dynamically combined mediators of short-distance host finding and acceptance. While sugars improve the capacity of rootworm larvae to find a host plant and to distinguish postembryonic from less nutritious embryonic roots, benzoxazinoids are specifically required for the latter. Host acceptance in the form of root damage is increased by benzoxazinoids and sugars in an additive manner. This pattern is driven by increasing damage to postembryonic roots in the presence of benzoxazinoids and sugars. Benzoxazinoid- and sugar-mediated foraging directly improves western corn rootworm growth and survival. Interestingly, western corn rootworm larvae retain a substantial fraction of their capacity to feed and survive on maize plants even when both classes of chemical cues are almost completely absent. This study unravels fine-grained differentiation and combination of primary and secondary metabolites into herbivore foraging and documents how the capacity to compensate for the lack of important chemical cues enables a specialist herbivore to survive within unpredictable metabolic landscapes.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The plant metabolome guides fitness-relevant foraging decisions of a specialist herbivore

et al. 2021

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“…In Plutella xylostella , GR34 was identified to recognize the plant hormone brassinolide using a single gene‐expressing oocyte (Yang et al ., 2020). A similar electrophysiological response was also observed in the fructose receptors GR8 and GR43a from Spodoptera litura and Trichogramma chilonis , respectively (Liu et al ., 2019a, 2019b). In contrast, some GRs showed they needed a coexpression form for function.…”

Section: Discussionmentioning

confidence: 99%

A fructose receptor gene influences development and feed intake in Helicoverpa armigera

Dong

Yang

et al. 2021

Insect Science

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Gustatory receptors (GRs) are critical for multiple life activities of insects. Owing to the rapid development of genome and transcriptome sequencing, numerous insect GRs have been identified. However, the expression patterns and functions of these receptors are poorly understood. In this study, we analyzed the expression pattern of GRs in Helicoverpa armigera and found that the fructose receptor HarmGR9 was highly expressed in the foregut and abdomen. The function of HarmGR9 was identified using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9) system. Knockout of the HarmGR9 gene shortened the developmental period of the larval stages and increased food consumption in both larvae and adults. This study revealed the tissue distribution of sugar‐sense‐related receptors in H. armigera and thereby expanded the understanding of insect feeding regulation.

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“…This approach was chosen because manipulation of primary metabolism in plants results in pleiotropic effects (73,74). Sugar perception in insects is highly conserved and mediated by Gr43a-like genes (49,50,53,54,(75)(76)(77)(78). BLAST search of publicly available nucleotide sequences revealed a single putative Gr43a gene in the western corn rootworm, here named DvvGr43a (Fig.…”

Section: Independent Manipulation Of Benzoxazinoids and Sugars As Formentioning

confidence: 99%

“…The full gene sequence can be retrieved from the National Center for Biotechnology Information (NCBI) data bank using the following accession number: XM_028279548.1. Then, we reconstructed evolutionary relationships between the identified DvvGr43a gene and several other putative Gr43a genes that have been functionally characterized as glucose, fructose, and/or sucrose receptors (49,50,53,54,(75)(76)(77)(78). The evolutionary relationships were inferred using the Maximum Likelihood method based on the Le Gascuel 2008 model in MEGA7 (101,102).…”

Section: Identification Of Dvvgr43amentioning

confidence: 99%

Complex plant metabolomes guide fitness-relevant foraging decisions of a specialist herbivore

Machado

Theepan

Robert

et al. 2020

Preprint

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Plants produce complex mixtures of primary and secondary metabolites. Herbivores use these metabolites as behavioral cues to increase their fitness. However, how herbivores integrate primary and secondary metabolites into fitness-relevant foraging decisions in planta is poorly understood. We developed a molecular manipulative approach to modulate the availability of sugars and benzoxazinoid secondary metabolites as foraging cues for a specialist maize herbivore, the western corn rootworm. By disrupting benzoxazinoid biosynthesis in maize and sugar perception in the western corn rootworm, we show that sugars and benzoxazinoids act as distinct and dynamically integrated mediators of short-distance host finding and acceptance. While sugars improve the capacity of rootworm larvae to find a host plant and to distinguish post-embryonic from less nutritious embryonic roots, benzoxazinoids are specifically required for the latter. Host acceptance in the form of root damage is increased by benzoxazinoids and sugars in an additive manner. This pattern is driven by increasing damage to post-embryonic roots in the presence of benzoxazinoids and sugars. Benzoxazinoid- and sugar-mediated foraging directly improves western corn rootworm growth and survival. Interestingly, western corn rootworm larvae retain a substantial fraction of their capacity to feed and survive on maize plants in the absence of both cues. This study unravels fine-grained differentiation and integration of primary and secondary metabolites into herbivore foraging and documents how the capacity to compensate for the absence of important chemical cues enables a specialist herbivore to survive within unpredictable metabolic landscapes.

show abstract

Identification and functional characterization of D-fructose receptor in an egg parasitoid, Trichogramma chilonis

Cited by 11 publications

References 71 publications

The plant metabolome guides fitness-relevant foraging decisions of a specialist herbivore

The plant metabolome guides fitness-relevant foraging decisions of a specialist herbivore

A fructose receptor gene influences development and feed intake in Helicoverpa armigera

Complex plant metabolomes guide fitness-relevant foraging decisions of a specialist herbivore

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