Identification and functional expression of tahA, a filamentous fungal gene involved in copper trafficking to the secretory pathway in Trametes versicolor

Uldschmid, Andreas; Engel, Michael; Dombi, Renate; Marbach, Karin

doi:10.1099/00221287-148-12-4049

Cited by 10 publications

(18 citation statements)

References 38 publications

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“…This method has been used in plants [28], fungi [29], worms [30], rats [31] and humans [32,33] and the involvement of these proteins in Cu + -homeostasis in their own organism was confirmed. This method has been used in plants [28], fungi [29], worms [30], rats [31] and humans [32,33] and the involvement of these proteins in Cu + -homeostasis in their own organism was confirmed.…”

Section: Discussionmentioning

confidence: 86%

Cd²⁺‐ or Hg²⁺‐binding proteins can replace the Cu⁺‐chaperone Atx1 in delivering Cu⁺ to the secretory pathway in yeast

Morin¹,

Cuillel²,

Lowe³

et al. 2005

FEBS Letters

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Copper delivery to Ccc2--the Golgi Cu+-ATPase--was investigated in vivo, replacing the Cu+-chaperone Atx1 by various structural homologues in an atx1-Delta yeast strain. Various proteins, displaying the same ferredoxin-like fold and (M/L)(T/S)CXXC metal-binding motif as Atx1 and known as Cu+-, Cd2+- or Hg2+-binding proteins were able to replace Atx1. Therefore, regardless of their original function, these proteins could all bind copper and transfer it to Ccc2, suggesting that Ccc2 is opportunistic and can interact with many different proteins to gain Cu+. The possible role of electrostatic potential surfaces in the docking of Ccc2 with these Atx1-homologues is discussed.

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Section: Discussionmentioning

confidence: 86%

Cd²⁺‐ or Hg²⁺‐binding proteins can replace the Cu⁺‐chaperone Atx1 in delivering Cu⁺ to the secretory pathway in yeast

Morin¹,

Cuillel²,

Lowe³

et al. 2005

FEBS Letters

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“…This result suggests the possibility that ctr1 expression is regulated by the presence of the metal. Regulation by copper has also been seen in the case of tahA gene of T. versicolor that codes for a copper chaperone protein (Uldschmid et al 2002). The expression of this gene is induced under elevated copper concentrations and repressed under copper starvation.…”

Section: Discussionmentioning

confidence: 89%

“…All copper transporters share structurally conserved domains such as three transmembrane regions, a variable number of methionines arranged as MxxM and MxM motifs at the N-terminal end, and a number of functionally important charged amino acids at the C-terminal end. In contrast to the case in ascomycetous fungi, such as S. cerevisiae, Candida albicans and Podospora anserina, copper homeostasis in basidiomycetes remains largely unexplored, and only two genes for P-type ATPases [ tahA (Uldschmid et al 2002) and ctaA (Uldschmid et al 2003)] involved in copper trafficking in the white rot fungus Trametes versicolor have been described.…”

Section: Introductionmentioning

confidence: 99%

Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter

et al. 2005

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Copper homeostasis is crucial for the maintenance of life. In lignin-degrading fungi, copper is essential for the phenol oxidase enzymes that provide this activity. In this paper we report the characterization of a gene (ctr1) coding for a copper transporter in the white rot fungus Pleurotus ostreatus. The gene was identified in a cDNA library constructed from 4-day-old vegetative mycelium grown in liquid culture. The results presented here demonstrate that: (1) ctr1 functionally complements the respiratory deficiency of a yeast mutant defective in copper transport, supporting the idea that the Ctr1 protein is itself a copper transporter; (2) transcription of ctr1 is detectable in P. ostreatus at all developmental stages and in all tissues (with the exception of lamellae), and is negatively regulated by the presence of copper in the culture medium; (3) ctr1 is a single-copy gene that maps to P. ostreatus linkage group III; and (4) the regulatory sequence elements found in the promoter of ctr1 are similar to those found in other copper-related genes described in other systems. These results provide the first description of a copper transporter in this white rot fungus and should be useful for further studies on copper metabolism in higher basidiomycetes.

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“…Uldschmid et al . confirmed the functions of ctaA in copper trafficking by overexpression and complementation of the corresponding S. cerevisiae ccc2 mutant (Uldschmid et al ., 2002; 2003).…”

Section: Resultsmentioning

confidence: 99%

“…To test whether CCC2 encodes a functional homologue in C. neoformans, mutant ST239D (ccc2) was comple-mented with its homologue ctaA from T. versicolor, a related basidiomycete. Uldschmid et al confirmed the functions of ctaA in copper trafficking by overexpression and complementation of the corresponding S. cerevisiae ccc2 mutant (Uldschmid et al, 2002;.…”

Section: Complementation Of the Ccc2 Mutation With The T Versicolor mentioning

confidence: 94%

Novel gene functions required for melanization of the human pathogen Cryptococcus neoformans

Walton

Idnurm

Heitman

2005

Molecular Microbiology

226

191

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SummaryThe ability to produce melanin is a key virulence factor in many fungal pathogens including the human basidiomycete pathogen Cryptococcus neoformans , a major cause of life-threatening infections among immunocompromised persons. Despite the significance of melanin biosynthesis in virulence of C. neoformans , the cellular and molecular processes involved in this pathway have not yet been fully elucidated. Here, we used Agrobacterium to isolate insertional mutants and screened 12 000 mutants to uncover genes involved in melanin production in C. neoformans. Four new mutant alleles of the wellknown melanin biosynthesis gene, LAC1 , which encodes laccase were identified, and the T-DNA was shown to have a possible predisposition for insertion into the promoters of genes, in particular LAC1. Melanization in C. neoformans is dependent on five additional genes identified in this screen encoding homologues of the copper transporter Ccc2, the copper chaperone Atx1, the chitin synthase Chs3, the transcriptional coactivator Mbf1 and the chromatinremodelling enzyme Snf5. Illumination of the molecular and genetic components of this virulence pathway reveals potential novel targets for drug development against C. neoformans and provides further insight into the intimate relationship between metal ion homeostasis and melanin biosynthesis.

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Identification and functional expression of tahA, a filamentous fungal gene involved in copper trafficking to the secretory pathway in Trametes versicolor

Cited by 10 publications

References 38 publications

Cd²⁺‐ or Hg²⁺‐binding proteins can replace the Cu⁺‐chaperone Atx1 in delivering Cu⁺ to the secretory pathway in yeast

Cd²⁺‐ or Hg²⁺‐binding proteins can replace the Cu⁺‐chaperone Atx1 in delivering Cu⁺ to the secretory pathway in yeast

Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter

Novel gene functions required for melanization of the human pathogen Cryptococcus neoformans

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Identification and functional expression of tahA, a filamentous fungal gene involved in copper trafficking to the secretory pathway in Trametes versicolor

Cited by 10 publications

References 38 publications

Cd2+‐ or Hg2+‐binding proteins can replace the Cu+‐chaperone Atx1 in delivering Cu+ to the secretory pathway in yeast

Cd2+‐ or Hg2+‐binding proteins can replace the Cu+‐chaperone Atx1 in delivering Cu+ to the secretory pathway in yeast

Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter

Novel gene functions required for melanization of the human pathogen Cryptococcus neoformans

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Cd²⁺‐ or Hg²⁺‐binding proteins can replace the Cu⁺‐chaperone Atx1 in delivering Cu⁺ to the secretory pathway in yeast

Cd²⁺‐ or Hg²⁺‐binding proteins can replace the Cu⁺‐chaperone Atx1 in delivering Cu⁺ to the secretory pathway in yeast