Identification and in vitro production of Lactobacillus antagonists from women with or without bacterial vaginosis

Branco, Kelly Moreira Grillo Ribeiro; Nardi, Roberta De; Moreira, J. L.; Nunes, Álvaro Cantini; Farias, Luiz M.; Nicoli, Jacques R.; Carvalho, Maria Auxiliadora Roque de

doi:10.1590/s0100-879x2010007500013

Cited by 11 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, as described in other studies (Hellberg et al, 2001;Branco et al, 2010;Srinivasan et al, 2010), G. vaginalis frequency was higher in patients with BV when compared to healthy women. However, when present in patients with BV, Lactobacillus levels were similar to those found in healthy women, suggesting that a beneficial effect of these bacteria depends not only on their presence in high quantities but also on their quality in term of protective ability.…”

Section: Discussionsupporting

confidence: 83%

“…Recently, our laboratory reported the production of synergistic and/or antagonistic compounds by G. vaginalis (Teixeira et al, 2010). In a second study, we confirmed the importance of high population levels of Lactobacillus (essentially Lactobacillus crispatus) and the quality of these lactobacilli as producers of antagonistic substances for a healthy vaginal environment (Branco et al, 2010). These results showed that the quantity of beneficial bacteria as well as their qualities as producers of antagonistic compounds seem to be pivotal factors in understanding BV and the ecological role of these bacteria in the vaginal environment.…”

Section: Introductionsupporting

confidence: 63%

See 1 more Smart Citation

Characteristics of Lactobacillus and Gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice

Teixeira

Carvalho

Arantes

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo using a gnotobiotic animal model challenged with one of the more sensitive G. vaginalis isolates. A total of 24 isolates from women with and without BV were identified as G. vaginalis. A higher frequency (P,0.05) of this bacterium was observed in women with BV (56.7 %) when compared to healthy women (17.6 %). A total of 86 strains of Lactobacillus were obtained from healthy women and women with BV. Lactobacillus strains were more frequently present (P,0.05) in healthy women (97.5 %) than in women with BV (76.7 %). Lactobacillus crispatus was the predominating strain in both healthy women and women with BV. Lactobacillus jensenii, Lactobacillus johnsonii, Lactobacillus gasseri and Lactobacillus vaginalis were isolated with an intermediate frequency in the two groups. In vitro antagonism assays were performed using as indicators 17 reference strains and the G. vaginalis strains isolated from women with BV and from healthy women. Lactobacillus isolated from healthy women showed the higher antagonistic activity against all the indicator strains when compared with isolates from women with BV. Concerning the indicator strains, G. vaginalis found in women with BV was more resistant to the antagonism, particularly when Lactobacillus isolates from women with BV were used as producer strains. A high vaginal population level of G. vaginalis was obtained by intravaginal inoculation of germ-free mice, and this colonization was accompanied by vaginal histopathological lesions. A tenfold decrease in vaginal population level of G. vaginalis and a reduction of histological lesions were observed when the pathogenic challenge was performed in mice previously monoassociated with an L. johnsonii strain. Concluding, results of the present study suggest that progression of G. vaginalis-associated BV depends in part on a simultaneous presence of Lactobacillus populations with a low antagonistic capacity and of a G. vaginalis strain with a high resistance to this antagonism. The results could also explain why G. vaginalis is frequently found in the vaginal ecosystem of healthy women.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Introductionsupporting

confidence: 63%

Characteristics of Lactobacillus and Gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice

Teixeira

Carvalho

Arantes

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…johnsoni , and L . iners [ 2 – 4 ]. Similar microbiota were described for monkeys and rats [ 5 , 6 ], but the vaginal microbiota in cows remains relatively unknown.…”

Section: Introductionmentioning

confidence: 99%

Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis

et al. 2015

View full text Add to dashboard Cite

Understanding of microbial communities inhabiting cattle vaginal tract may lead to a better comprehension of bovine physiology and reproductive health being of great economic interest. Up to date, studies involving cattle microbiota are focused on the gastrointestinal tract, and little is known about the vaginal microbiota. This study aimed to investigate the vaginal microbiome in Nellore cattle, heifers and cows, pregnant and non-pregnant, using a culture independent approach. The main bacterial phyla found were Firmicutes (~40–50%), Bacteroidetes (~15–25%) and Proteobacteria (~5–25%), in addition to ~10–20% of non-classified bacteria. 45–55% of the samples were represented by only ten OTUs: Aeribacillus, Bacteroides, Clostridium, Ruminococcus, Rikenella, Alistipes, Bacillus, Eubacterium, Prevotella and non-classified bacteria. Interestingly, microbiota from all 20 animals could be grouped according to the respiratory metabolism of the main OTUs found, creating three groups of vaginal microbiota in cattle. Archaeal samples were dominated by the Methanobrevibacter genus (Euryarchaeota, ~55–70%). Ascomycota was the main fungal phylum (~80–95%) and Mycosphaerella the most abundant genus (~70–85%). Hormonal influence was not clear, but a tendency for the reduction of bacterial and increase of archaeal populations in pregnant animals was observed. Eukaryotes did not vary significantly between pregnant and non-pregnant animals, but tended to be more abundant on cows than on heifers. The present work describes a great microbial variability in the vaginal community among the evaluated animals and groups (heifers and cows, pregnant and non-pregnant), which is significantly different from the findings previously reported using culture dependent methods, pointing out the need for further studies on this issue. The microbiome found also indicates that the vaginal colonization appears to be influenced by the gastrointestinal community.

show abstract

“…Residual chloroform was allowed to evaporate, and Petri dishes were overlaid with 3.5 mL of a soft agar containing brain heart infusion (Acumedia, Neogen Co., Lansing, MI, USA), tryptone soy broth (Difco) supplemented with 0.5% yeast extract (Acumedia), or Ellinghausen–McCullogh–Johnson–Harris with Leptospira enrichment EMJH (Difco) inoculated with 0.2 mL of a 24 h culture of Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 19433, Pseudomonas aeruginosa ATCC 25853, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25723, Salmonella enterica serovar Typhimurium ATCC 14028, Leptospira interrogans serovar Icterohaemorrhagiae , or Listeria monocytogenes ATCC 15313. After incubating at 37°C for 24 h under aerobic or anaerobic conditions, depending on the indicator strain, the antagonistic activity was determined based on the presence of a growth inhibition zone, using the method of Tagg as modified by Branco et al ., (2010) [100]. …”

Section: Methodsmentioning

confidence: 99%

Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays

et al. 2017

View full text Add to dashboard Cite

Lactococcus lactis subsp. lactis NCDO 2118 was recently reported to alleviate colitis symptoms via its anti-inflammatory and immunomodulatory activities, which are exerted by exported proteins that are not produced by L. lactis subsp. lactis IL1403. Here, we used in vitro and in silico approaches to characterize the genomic structure, the safety aspects, and the immunomodulatory activity of this strain. Through comparative genomics, we identified genomic islands, phage regions, bile salt and acid stress resistance genes, bacteriocins, adhesion-related and antibiotic resistance genes, and genes encoding proteins that are putatively secreted, expressed in vitro and absent from IL1403. The high degree of similarity between all Lactococcus suggests that the Symbiotic Islands commonly shared by both NCDO 2118 and KF147 may be responsible for their close relationship and their adaptation to plants. The predicted bacteriocins may play an important role against the invasion of competing strains. The genes related to the acid and bile salt stresses may play important roles in gastrointestinal tract survival, whereas the adhesion proteins are important for persistence in the gut, culminating in the competitive exclusion of other bacteria. Finally, the five secreted and expressed proteins may be important targets for studies of new anti-inflammatory and immunomodulatory proteins. Altogether, the analyses performed here highlight the potential use of this strain as a target for the future development of probiotic foods.

show abstract

Identification and in vitro production of Lactobacillus antagonists from women with or without bacterial vaginosis

Cited by 11 publications

References 24 publications

Characteristics of Lactobacillus and Gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice

Characteristics of Lactobacillus and Gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice

Vaginal Microbiome Characterization of Nellore Cattle Using Metagenomic Analysis

Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays

Contact Info

Product

Resources

About