Identification and iterative combinatorial mutagenesis of a new naringinase‐producing strain,Aspergillus tubingensisMN589840

Abstract

Naringinase was mainly obtained by microbial fermentation, and mutagenesis was a major way for obtaining excellent mutants. The aim of this study was to screen out a high naringinase yielding mutant to enhance the potential application value of its industrialization and compare the effects of different mutagenic methods on the enzyme activity of the strain. A novel producing naringinase strain, Aspergillus tubingensis MN589840, was isolated from mildewed pomelo peel, later subjected to mutagenesis including UV, ARTP and UV‐ARTP. After five rounds iterative mutagenesis, the mutants U1, A6 and UA13 were screened out with 1448·49, 1848·71, 2475·16 U mg−1 enzyme activity, the naringinase productivity raised by 79·08, 123·56 and 206%, respectively. In addition, the naringinase activity of three mutants rose after each round of iterative mutagenesis. These results indicated that the mutagenesis efficiency of UV‐ARTP was higher than that of single ARTP, and both are better than UV. In summary, the iterative UV‐ARTP mutagenesis is an effective strategy for screening high naringinase‐producing strains.

Improvement of macrolactins production by the genetic adaptation of Bacillus siamensis A72 to saline stress via adaptive laboratory evolution

“…Previous study revealed that combined multiple mutagenesis strategy (e.g., UV mutagenesis, ARTP, or ALE) had higher efficiency than that of a single one [ 59 , 60 ]. In addition, the combination of ARTP mutagenesis and ALE has been successfully applied to breed desired phenotype of strains [ 61 , 62 ].…”

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

“…To address the bottleneck of reduced relative l ‐serine yield, Zhang et al, 106 performed ARTP mutagenesis based on engineered modified Corynebacterium glutamicum , which was found to substantially enhance l ‐serine yield. In addition, Xia et al, 107 performed mutagenesis with UV, ARTP, and UV‐ARTP, respectively, and found that the mutagenesis efficiency of UV‐ARTP was higher than that of a single ARTP and all were superior to that of UV (The yields of target products were increased by 79.08%, 123.56%, and 206% after UV, ARTP, and UV‐ARTP mutagenesis, respectively).…”

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

“…For instance, naringinase, an enzyme with wide applications in the food, beverage, and pharmaceutical industries, saw significant productivity increases through UV-ARTP mutagenesis in Aspergillus tubingensis MN589840. High-yield mutants U1, A6, and UA13 exhibited impressive productivity enhancements [ 88 ]. Furthermore, ARTP has been successfully utilized for the production of diverse bioenzymes, including tannase [ 89 , 90 ], raw starch-degrading enzymes [ 91 ], thermostable xylanase [ 92 ], cellulose [ 93 , 94 ] and L-asparaginase [ 95 ].…”