Identification and mapping of a new leaf stripe resistance gene in barley (Hordeum vulgare L.)

Tacconi, G.; Cattivelli, Luigi; Faccini, N.; Pecchioni, Nicola; Stanca, A. M.; Valè, Giampiero

doi:10.1007/s001220000510

Cited by 36 publications

(36 citation statements)

References 16 publications

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“…The genotype for the Rdg2a locus was determined for all F 2 individuals (mediumresolution population) or for selected F 2 recombinants (highresolution population), by performing leaf stripe resistance assays on F 3 progeny, essentially as described by Tacconi et al (2001). Isolate Dg2, which is avirulent with respect to the resistance gene Rdg2a, was used to inoculate germinating seeds artificially.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, the Rdg2a-linked RAPD fragment SCQ-9 700 was cloned and sequenced, a specific pair of primers made, and the primers used to amplify and sequence the fragment from the mapping population parents, Thibaut and Mirco (Tacconi et al 2001). Since no sequence polymorphism was identified within the fragment, we recovered additional sequence flanking the original fragment, by using an adapter-mediated PCR method (Siebert et al 1995).…”

Section: Pcr Marker Developmentmentioning

confidence: 99%

“…Rdg1a, conferring complete resistance to a subset of P. graminea isolates, has been mapped on the long arm of barley chromosome 2 (2H) (Giese et al 1993; Thomsen et al 1997). Rdg2a, conferring complete resistance to the most virulent Italian isolate of P. graminea (Dg2), has been mapped to barley chromosome 1 (7H) (Tacconi et al 2001). Plants containing Rdg2a are almost immune to disease caused by avirulent isolates, exhibiting no brown stripes on the leaves.…”

Section: Introductionmentioning

confidence: 99%

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High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

et al. 2003

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The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F(2) population of 1,400 plants (Thibaut Rdg2axMirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein.

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Section: Methodsmentioning

confidence: 99%

Section: Pcr Marker Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

et al. 2003

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“…Apart from Rpg1, it contained five barley pic20 homologues, the NBS-LRR type RGA named B9 (Leister et al 1998), the pic15 locus containing RGAs pic15, pic25, pic25-1 and pic27 (Collins et al 2001), as well as several other RGAs (Brueggeman and Kleinhofs, unpublished). Recently Tacconi et al (2001) mapped a new barley leaf stripe resistance gene Rdg2a to chromosome 1(7H) 2.5 cM distal to the RFLP marker MWG2018 and 5.8 cM distal to RFLP locus ABG704. However, in the NABGMP Steptoe × Morex DHL mapping population MWG2018 mapped approximately 3.4 cM proximal to ABG704.…”

Section: Discussionmentioning

confidence: 99%

“…However, in the NABGMP Steptoe × Morex DHL mapping population MWG2018 mapped approximately 3.4 cM proximal to ABG704. Since both probes hybridized to several bands it is possible that one or both were mapped to a different locus by Tacconi et al (2001). Due to the genetic proximity, it is nevertheless possible that the barley pic20 homologues could represent candidate genes for Rdg2a.…”

Section: Discussionmentioning

confidence: 99%

A barley gene family homologous to the maize rust resistance gene Rp1-D

et al. 2002

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Many characterized plant disease resistance genes encode proteins which have conserved motifs such as the nucleotide binding site. Conservation extends across different species, therefore resistance genes from one species can be used to isolate homologous regions from another by employing DNA sequences encoding conserved protein motifs as probes. Here we report the isolation and characterization of a barley ( Hordeum vulgare L.) resistance gene analog family consisting of nine members homologous to the maize rust resistance gene Rp1-D. Five barley Rp1-D homologues are clustered within approximately 400 kb on chromosome 1(7H), near, but not co-segregating with, the barley stem rust resistance gene Rpg1; while others are localized on chromosomes 3(3H), 5(1H), 6(6H) and 7(5H). Analyses of predicted amino-acid sequences of the barley Rp1-D homologues and comparison with known plant disease resistance genes are presented.

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Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

et al. 2021

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Tibetan hulless barley (Hordeum vulgare L. variety nudum Hook. f.) is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Here, we compared the miRNA profiles before and after BLS in a BLS-sensitive ('Z1141') and tolerant ('Kunlun14') Tibetan hulless barley for the first time. A total of 36 conserved and 56 novel miRNAs were identified, of which 24 differentially expressed miRNAs were found in Z1141 and Kunlun14, and an additional 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among these genes, 131 were differentially expressed in normal and infected leaves of Kunlun14 and Z1141.Gene ontology (GO), pathway, and Blast analyses indicated 10 candidate target genes including seven functional genes and three transcription factors were involved in barley disease resistance. These functional genes were two members of the CYP450 genes, a RGA gene, a LIN gene, a SAM gene, a PSD gene, and a NDB gene; the three transcription factor genes were all from the WRKY family. These 10 candidate target genes were regulated by eight miRNAs (hvu-miR168-3p, hvu-miR171-5p, hvu-miR159b, hvu-miR156a, hvu-novel-91, hvu-novel-46, hvu-novel-52, and hvu-novel-11). According to quantitative real-time polymerase chain reaction (PCR) results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Thus, these miRNAs and their target genes can be used to improve BLS resistance in Tibetan hulless barley though breeding programs or genetic engineering.

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Identification and mapping of a new leaf stripe resistance gene in barley (Hordeum vulgare L.)

Cited by 36 publications

References 16 publications

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley

A barley gene family homologous to the maize rust resistance gene Rp1-D

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

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