Identification and Mapping of Amplified Fragment Length Polymorphism Markers Linked to Shell Color in Bay Scallop, Argopecten irradians irradians (Lamarck, 1819)

Abstract

Amplified fragment length polymorphisms (AFLP) were used to study the inheritance of shell color in Argopecten irradians. Two scallops, one with orange and the other with white shells, were used as parents to produce four F(1) families by selfing and outcrossing. Eighty-eight progeny, 37 orange and 51 white, were randomly selected from one of the families for segregation and mapping analysis with AFLP and microsatellite markers. Twenty-five AFLP primer pairs were screened, yielding 1138 fragments, among which 148 (13.0%) were polymorphic in two parents and segregated in progeny. Six AFLP markers showed significant (P < 0.05) association with shell color. All six loci were mapped to one linkage group. One of the markers, F1f335, is completely linked to the gene for orange shell, which we designated as Orange1, without any recombination in the progeny we sampled. The marker was amplified in the orange parent and all orange progeny, but absent in the white parent and all the white progeny. The close linkage between F1f335 and Orange1 was validated using bulk segregation analysis in two natural populations, and all our data indicate that F1f335 is specific for the shell color gene, Orange1. The genomic mapping of a shell color gene in bay scallop improves our understanding of shell color inheritance and may contribute to the breeding of molluscs with desired shell colors.

Construction of a high-density genetic map and QTL mapping for pearl quality-related traits in Hyriopsis cumingii

“…For instance, QTL mapping for growth traits and disease resistance has been conducted in the Pacific oyster2829; QTL mapping for growth and shell colour has been conducted in Argopecten irradians 3031; and QTL mapping for growth traits and sex-determination has been conducted in Chlamys farreri 7. In addition, for some pearl mussels, QTLs have been identified for growth and shell colour in the freshwater mussel H. cumingii 32, and QTLs for pearl quality and shell growth have been identified in Pinctada maxima 3334.…”

A Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis

“…The shell color was determined according to the color system developed by Elek and Adamkewicz [32] in this study. As previous studies indicated that shell color in the bay scallop is under single-gene control where white color is recessive [33], [34], the genotypes for orange-colored and white-colored parent were recorded as heterozygote and homozygote respectively.…”

Identification of Single-Locus PCR-Based Markers Linked to Shell Background Color in the Pacific Oyster (Crassostrea gigas)

“…This can significantly reduce the cost of producing breeding lines and can accelerate the breeding program greatly. Several genetic markers associated with shell color have been identified in aquaculture species such as bay scallop (Qin et al 2007), Pacific lion-paw scallop (Petersen et al 2012), and noble scallop (Yuan et al 2012). A SCAR marker which was well correlated with Pacific oyster shell pigmentation of light and dark was also identified, suggesting a major locus that is responsible for foreground pigmentation (Ge et al 2014).…”

“…The amplified fragment length polymorphism (AFLP) technique is a powerful marker system (Vos et al 1995), which has been used extensively for linkage mapping and gene/QTL mapping in marine organisms (Yu and Guo 2003;Qin et al 2007;Guo et al 2012;Yuan et al 2012). The bulked segregant analysis (BSA), developed by Michelmore et al (1991), has been proved to be an effective method for detecting markers that are tightly linked with genes in many aquatic species (Nakamura et al 2001;Araneda et al 2005Araneda et al , 2009Felip et al 2005;Liu et al 2011;Ge et al 2014;Wang et al 2014).…”