Identification and Mapping of QTLs for Sulfur-Containing Amino Acids in Soybean (Glycine max L.)

Abstract

Soybean is a major source of high-quality protein for humans and animals. The content of sulfur-containing amino acids (SAA) in soybean is insufficient, which has become the main factor limiting soybean nutrition. In this study, we used the high-density genetic maps derived from Guizao 1 and Brazil 13 to evaluate the quantitative trait loci of cysteine (Cys), methionine (Met), SAA, glycinin (7S), β-conglycinin (11S), ratio of glycinin to β-conglycinin (RGC), and protein content (PC). In genetic map linkage analysis, the major and stable 44 QTLs were detected, which shared nine bin intervals. Among them, the bin interval (bin157-bin160) on chromosome 5 was detected in multiple environments as a stable QTL, which was linked to 11S, 7S, RGC, and SSA. Based on the analysis of bioinformatics and RNA-sequencing data, 16 differentially expressed genes (DEGs) within these QTLs were selected as candidate genes. These results will help to elucidate the genetic mechanism of soybean SAA-related traits and provide the basis for the gene mining of sulfur-containing amino acids.

Bioaccessibility and Digestibility of Proteins in Plant-Based Drinks and Cow’s Milk: Antioxidant Potential of the Bioaccessible Fraction

“…Soybean proteins have been reported to contain low levels of sulfuric amino acids. 49 Rutherfurd et al reported a slightly lower DIAAS (using the amino acid requirement pattern for the 0.5−3-y-old child) of 91% for amino acids in a soy protein isolate. 48 The quality of the essential amino acids in cow's milk and soy drinks is therefore considered excellent.…”

“…Also, the soy drink with a DIAAS of 100% for sulfur amino acids could still be considered an excellent protein source. Soybean proteins have been reported to contain low levels of sulfuric amino acids . Rutherfurd et al reported a slightly lower DIAAS (using the amino acid requirement pattern for the 0.5–3-y-old child) of 91% for amino acids in a soy protein isolate .…”

Natural variation of GmFATA1B regulates seed oil content and composition in soybean

“…The DNA polymorphism datasets were obtained from public data (Zhou et al, 2015; Fang et al, 2017; Liu et al, 2020; Lu et al, 2020; Torkamaneh et al, 2021; Qi et al, 2021b; Guo et al, 2022; Zhong et al, 2023) and used for genetic polymorphism analysis of GmFATA1B . For haplotype homozygosity analysis, the SNPs with minor allele frequency (maf) >5% and max missing <10% were used.…”

Folate Biofortification in Soybean: Challenges and Prospects

“…High-throughput genotyping methods, such as genotyping by sequencing (GBS), can be used to genotype a large number of accessions [102], while phenotyping methods can be used to measure the folate content of these accessions. Linkage mapping, genomewide association studies (GWAS) [103], and bulked segregant analysis-sequencing (BSA-Seq) [104] can then be used to identify quantitative trait loci (QTL) [105] or quantitative trait nucleotides (QTN) [106] that are associated with folate content. RNA-Seq can be combined with identified QTL or QTN and analysed using weighted gene co-expression network analysis (WGCNA) to group genes with similar expression patterns and identify candidate genes [107].…”