Identification and Optimization of Carbon Radicals on Hydrated Graphene Oxide for Ubiquitous Antibacterial Coatings

Li, Ruibin; Mansukhani, Nikhita D.; Guiney, Linda M.; Ji, Zhaoxia; Zhao, Yichao; Chang, Chong Hyun; French, Christopher T.; Miller, Jeff F.; Hersam, Mark C.; Nel, André E.; Xia, Tian

doi:10.1021/acsnano.6b05692

Cited by 186 publications

(182 citation statements)

References 47 publications

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“…Due to the fact that 1 O 2 species are highly reactive with an extremely short half-life within tens of nanoseconds, the distance allowing for 1 O 2 diffusion in cytoplasm is limited. [19] However, the highly oxidative nature of 1 O 2 species may cause immediate oxidation of cytoplasmic substances nearby, thus elevating the intracellular ROS level. In this respect, we studied the changes of ROS level of cells treated with IO-LAHP NPs using a 2′,7′-dichlorodihydrofluorescein diacetate probe (H 2 DCFDA).…”

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confidence: 99%

Activatable Singlet Oxygen Generation from Lipid Hydroperoxide Nanoparticles for Cancer Therapy

et al. 2017

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Reactive oxygen species (ROS) induced apoptosis is a widely practiced strategy for cancer therapy. Although photodynamic therapy (PDT) takes advantages of spatial-temporal control of ROS generation, the meticulous participation of light, photosensitizer, and oxygen greatly hinders the broad application of PDT as a first-line cancer treatment option. Here, we developed an activatable system enabling tumor-specific singlet oxygen (1O2) generation for cancer therapy, based on a Fenton-like reaction between linoleic acid hydroperoxide (LAHP) tethered on iron oxide nanoparticles (IO NPs) and the released iron(II) ions from IO NPs under acidic-pH condition. We show that the IO-LAHP NPs are able to induce efficient apoptotic cancer cell death both in vitro and in vivo through tumor-specific 1O2 generation and subsequent ROS mediated mechanism. This study demonstrates the effectiveness of modulating biochemical reactions as a ROS source to exert cancer death, which may pave the way to develop novel strategies for cancer therapy.

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confidence: 99%

Activatable Singlet Oxygen Generation from Lipid Hydroperoxide Nanoparticles for Cancer Therapy

et al. 2017

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“…The Image-iT Lipid Peroxidation Assay enables the detection of lipid peroxidation in live cells through oxidation of BODIPY 581/591 C11 reagent, which will display a shift in peak fluorescence emission from ~590 nm to ~510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation [19, 61]. These assays provide simple and sensitive approaches and can be adapted easily to high-throughput applications.…”

Section: Fundamental Elements To Establish a Predictive Toxicologimentioning

confidence: 99%

Creative use of analytical techniques and high-throughput technology to facilitate safety assessment of engineered nanomaterials

2018

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With the rapid development and numerous applications of engineered nanomaterials (ENMs) in science and technology, their impact on environmental health and safety should be considered carefully. This requires an effective platform to investigate the potential adverse effects and hazardous biological outcomes of numerous nanomaterials and their formulations. We consider predictive toxicology a rational approach for this effort, which utilizes mechanism-based in vitro high-throughput screening (HTS) to make predictions on ENMs' adverse outcomes in vivo. Moreover, this approach is able to link the physicochemical properties of ENMs to toxicity that allows the development of structure-activity relationships (SARs). To build this predictive platform, extensive analytical and bioanalytical techniques and tools are required. In this review, we described the predictive toxicology approach and the accompanying analytical and bioanalytical techniques. In addition, we elaborated several successful examples as a result of using the predictive approach.

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“…85 In addition, they could attach to cell membrane as a 2D planar material and induce membrane damage and cytotoxicity. 86 One possible approach to reduce ECN cytotoxicity is surface functionalization. Wang et al 83 demonstrated that coating multiwall CNTs with a tri-block copolymer (Pluronic F108) mitigates their toxicity by preventing the lysosomal membrane damage and subsequent inflammatory pathways.…”

Section: Carbonaceous Nanomaterialsmentioning

confidence: 99%

Facilitating Translational Nanomedicine via Predictive Safety Assessment

et al. 2017

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Extensive research on engineered nanomaterials (ENMs) has led to the development of numerous nano-based formulations for theranostic purposes. Although some nano-based drug delivery systems already exist on the market, growing numbers of newly designed ENMs exhibit improved physicochemical properties and are being assessed in preclinical stages. While these ENMs are designed to improve the efficacy of current nanobased therapeutic or imaging systems, it is necessary to thoroughly determine their safety profiles for successful clinical applications. As such, our aim in this mini-review is to discuss the current knowledge on predictive safety and structure-activity relationship (SAR) analysis of major ENMs at the developing stage, as well as the necessity of additional long-term toxicological analysis that would help to facilitate their transition into clinical practices. We focus on how the interaction of these nanomaterials with cells would trigger signaling pathways as molecular initiating events that lead to adverse outcomes. These mechanistic understandings would help to design safer ENMs with improved therapeutic efficacy in clinical settings.During the past decades, engineered nanomaterials (ENMs) have been widely used in biomedical applications, such as nanomedicine, bioimaging, and tissue engineering, because of their unique biophysicochemical properties.1,2 With regard to nanomedicine applications, ENMs could be formulated as drug carriers that deliver the therapeutic reagents within the human body and increase their bioavailability and blood circulation half-life.2 Moreover, these nanocarriers could be functionalized to deliver the drug specifically to the desired target tissues (e.g., tumors) and release the payload sustainably over long periods, thereby eliminating the necessity of multiple drug administration and reducing its cytotoxic side effects toward healthy cells.2 In addition to their implementation in drug delivery, ENMs could be used for diagnostic and imaging purposes that would help to monitor the disease and administer the treatment more accurately. 2Past research in nanomedicine has led to the design of various nanomaterial-based therapeutic and diagnostic systems that are being investigated in experimental stages (e.g., in vitro and in vivo) and clinical trials or are commercially available to the corresponding patients (Table 1). Most commercialized nanomaterial-based therapeutic reagents use lipids, proteins, and polymers that could self-assemble and biodegrade with few side effects.3 Because of high biocompatibility of lipids, several commercial liposome-drug formulations have been developed, mostly relying on polyethylene glycol (PEG)-conjugated lipids, such as DOXIL, AmBisome, Epaxal, and Inflexal V, and are under clinical trials focusing on various types of disease treatments. 4,5 Polymer-based nanoparticles (NPs) also present low toxicity, but their surface functionalization may affect their safety. The most commonly used materials include PEG, ploy(lactic-co-glycolic) acid (PLGA...

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Identification and Optimization of Carbon Radicals on Hydrated Graphene Oxide for Ubiquitous Antibacterial Coatings

Cited by 186 publications

References 47 publications

Activatable Singlet Oxygen Generation from Lipid Hydroperoxide Nanoparticles for Cancer Therapy

Activatable Singlet Oxygen Generation from Lipid Hydroperoxide Nanoparticles for Cancer Therapy

Creative use of analytical techniques and high-throughput technology to facilitate safety assessment of engineered nanomaterials

Facilitating Translational Nanomedicine via Predictive Safety Assessment

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