Identification and ovarian developmental regulation of Insulin-like growth factor 3 in spotted scat (Scatophagus argus)

Li, Zhiyuan; Xilin, Ren; Guo, Yansu; Ru, Xiaoying; Tian, Changxu; Shi, Hongjuan; Jiang, Menghui; Zhu, Chunhua; Li, Guangli; Chen, Huapu

doi:10.1016/j.aqrep.2021.100866

Cited by 3 publications

(4 citation statements)

References 47 publications

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“…In our results, rdh12 expression was also decreased in the ZS group. Researchers have identified several genes that encode crucial enzymes in the steroidogenesis pathway, including cyp19a1a , cyp17a1 , and hsd17b1 , which could synthesize estradiol and progesterone, and play an important role in ovarian development and reproduction in fish ( Fang et al, 2019 ; Li et al, 2021 ). Transcriptomic analysis showed that cyp19a1a , cyp17a1 , and hsd17b1 were also downregulated in the ZS group compared to the NS group.…”

Section: Discussionmentioning

confidence: 99%

Effects of short-term water velocity stimulation on the biochemical and transcriptional responses of grass carp (Ctenopharyngodon idellus)

Shu,

Chen,

Xiao

et al. 2023

Front. Physiol.

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Since 2011, ecological operation trials of the Three Gorges Reservoir (TGR) have been continuously conducted to improve the spawning quantity of the four major Chinese carp species below the Gezhouba Dam. In particular, exploring the effects of short-term water velocity stimulation on ovarian development in grass carp (Ctenopharyngodon idellus) is essential to understand the response of natural reproduction to ecological flows. We performed ovary histology analysis and biochemical assays among individuals with or without stimulation by running water. Although there were no obvious effects on the ovarian development characteristics of grass carp under short-term water velocity stimulation, estradiol, progesterone, follicle-stimulating hormone (FSH), and triiodothyronine (T3) concentrations were elevated. Then, we further explored the ovarian development of grass carp under short-term water velocity stimulation by RNA sequencing of ovarian tissues. In total, 221 and 741 genes were up- or downregulated under short-term water velocity stimulation, respectively, compared to the control group. The majority of differentially expressed genes (DEGs) were enriched in pathways including ABC transporters, cytokine-cytokine receptor interaction, ECM-receptor interaction, and steroid hormone biosynthesis. Important genes including gpr4, vtg1, C-type lectin, hsd17b1, cyp19a1a, cyp17a1, and rdh12 that are involved in ovarian development were regulated. Our results provide new insights and reveal potential regulatory genes and pathways involved in the ovarian development of grass carp under short-term water velocity stimulation, which may be beneficial when devising further ecological regulation strategies.

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Section: Discussionmentioning

confidence: 99%

Effects of short-term water velocity stimulation on the biochemical and transcriptional responses of grass carp (Ctenopharyngodon idellus)

Shu,

Chen,

Xiao

et al. 2023

Front. Physiol.

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“…Oocyte development and maturation, essential processes for successful reproduction, play critical roles in artificial breeding in the aquaculture industry (41). Research has revealed that IGF3 can stimulate oocyte development and maturation in teleosts (24)(25)(26)(27). The molecular regulatory mechanism underlying IGF3-induced oocyte maturation remains unclear; accordingly, a RNA-seq analysis was performed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental methods are described in a previous study (25). In brief, the open reading frame of igf3 in S. argus was cloned.…”

Section: Experimental Treatment and Ethics Statementmentioning

confidence: 99%

“…IGF3 is a newly discovered member of the IGF family exclusively found in teleosts (23). It can stimulate ovarian development and maturation in teleosts (24)(25)(26)(27). Furthermore, the knockdown of igf3 in Cyprinus carpio via RNA interference significantly altered ncRNA expression profiles (28), indicating that ncRNAs are involved in IGF3-induced oocyte maturation; however, the molecular regulatory mechanism has not been determined.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of circRNA and miRNA expression profiles in IGF3-induced ovarian maturation in spotted scat (Scatophagus argus)

Guo²,

Ndandala³

et al. 2022

Front. Endocrinol.

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Insulin-like growth factor 3 (IGF3) induces ovarian maturation in teleosts; however, research on its molecular regulatory mechanism remains deficient. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in various biological processes, including reproduction. In this study, circRNAs and miRNAs involved in IGF3-induced ovarian maturation were evaluated in spotted scat (Scatophagus argus). In ovarian tissues, we identified 176 differentially expressed (DE) circRNAs and 52 DE miRNAs between IGF3 treatment and control groups. Gene Ontology (GO) enrichment analyses showed that host genes of DE circRNAs and target genes of DE miRNAs were enriched for various processes with a high degree of overlap, including cellular process, reproduction, reproductive process, biological adhesion, growth, extracellular region, cell junction, catalytic activity, and transcription factor activity. Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included cell adhesion molecules, ECM–receptor interaction, regulation of actin cytoskeleton, focal adhesion, cell cycle, Hedgehog signaling pathway, phosphatidylinositol signaling system, PI3K-Akt signaling pathway, Apelin signaling pathway, Notch signaling pathway, insulin signaling pathway, and Rap1 signaling pathway. A circRNA–miRNA–mRNA regulatory network was constructed, including DE genes involved in reproduction (e.g., oocyte maturation, oocyte meiosis, and ECM remodeling), such as ccnd2, hecw2, dnm2, irs1, adam12, and cdh13. According to the regulatory network and tissue distribution, we identified one circRNA (Lachesis_group5:6245955|6270787) and three miRNAs (novel_miR_622, novel_miR_980, and novel_miR_64) that may exert regulatory effects in IGF3-induced ovarian maturation in S. argus. Taken together, this study provides a novel insight into the molecular mechanisms by which IGF3 functions in ovaries and highlights the effects of circRNAs and miRNAs in reproduction in S. argus.

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Evolution and gene expression of matrix metalloproteinase gene family during gonadal development in Scatophagus argus

Zheng

2024

Am J Transl Res

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Identification and ovarian developmental regulation of Insulin-like growth factor 3 in spotted scat (Scatophagus argus)

Cited by 3 publications

References 47 publications

Effects of short-term water velocity stimulation on the biochemical and transcriptional responses of grass carp (Ctenopharyngodon idellus)

Effects of short-term water velocity stimulation on the biochemical and transcriptional responses of grass carp (Ctenopharyngodon idellus)

Analysis of circRNA and miRNA expression profiles in IGF3-induced ovarian maturation in spotted scat (Scatophagus argus)

Evolution and gene expression of matrix metalloproteinase gene family during gonadal development in Scatophagus argus

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