Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars

Brande, Stijn Van den; Gijbels, M. J. M.; Wynant, Niels; Peeters, Paulien; Gansemans, Yannick; Nieuwerburgh, Filip Van; Santos, Dulce; Broeck, Jozef Vanden

doi:10.1016/j.cris.2022.100041

Cited by 3 publications

(1 citation statement)

References 106 publications

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“…IGRs have been proposed as insect control agents 22 as they harbour compounds that impede insect growth by interfering with chitin synthesis or mimicking the juvenile hormone (JH), 20-hydroxyecdsyone hormone and moulting hormone. 23 That is why this class of chemicals is known to kill insects more slowly than conventional insecticides. 24 IGRs interfere with the moulting process, metamorphosis processes, 25 normal growth and development, 26 resulting in abnormalities in the insect.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of chemosterility effect of different insect growth regulators on Bactrocera zonata population

et al. 2023

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The indiscriminate application of synthetic pesticides on horticultural plants for the management of Bactrocera zonata has both economic and environmental implications due to the biomagnification of harmful residues in humans via the food chain. This necessitates the use of some eco-friendly control measures such as insect growth regulators (IGRs) as an alternative. A laboratory experiment was laid out to determine the potential chemosterilant effect of five IGRs including pyriproxyfen, novaluron, lufenuron, buprofezin, and flubendiamide at six different concentrations on B. zonata after treating adult diet. Using the oral bioassay, B. zonata were fed on IGRs impregnated diet (50–300 ppm/5 mL diet) which was replaced with the normal diet after 24 h of feeding. Ten pairs of B. zonata were placed in a separate plastic cage accommodating an ovipositor attractant guava for eggs collection and calculation. An analysis of the result revealed that fecundity and hatchability were higher at a low dose and vice versa. Lufenuron at 300 ppm/5 mL of diet significantly decreased the fecundity rate (31.1%) as compared to pyriproxyfen, novaluron, buprofezin and flubendiamide, which had 39.3%, 39.3%, 43.8%, and 47.5%, respectively. The lowest hatchability (19.9%) was noted in lufenuron treated diet followed by pyriproxyfen, novaluron, buprofezin, and flubendiamide, which had 22.1%, 25.0%, 30.9%, and 31.6%, respectively. Furthermore, in a population of crosses between the lufenuron treated male and female, a significant decrease in fecundity (45.5%) and hatchability (51.7%) was noted as compared to other IGRs. Overall, this study identified the chemosterilant potential of lufenuron on the population of B. zonata which can be integrated for its management strategy.

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Section: Introductionmentioning

confidence: 99%

Evaluation of chemosterility effect of different insect growth regulators on Bactrocera zonata population

et al. 2023

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Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease

Santiago,

da Silva Bentes,

da Silva

et al. 2023

Sci Rep

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The growing interest in microRNAs (miRNAs) over recent years has led to their characterization in numerous organisms. However, there is currently a lack of data available on miRNAs from triatomine bugs (Reduviidae: Triatominae), which are the vectors of the protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease. A comprehensive understanding of the molecular biology of vectors provides new insights into insect-host interactions and insect control approaches, which are key methods to prevent disease incidence in endemic areas. In this work, we describe the miRNome profiles from gut, hemolymph, and salivary gland tissues of the Rhodnius prolixus triatomine. Small RNA sequencing data revealed abundant expression of miRNAs, along with tRNA- and rRNA-derived fragments. Fifty-two mature miRNAs, previously reported in Ecdysozoa, were identified, including 39 ubiquitously expressed in the three tissues. Additionally, 112, 73, and 78 novel miRNAs were predicted in the gut, hemolymph, and salivary glands, respectively. In silico prediction showed that the top eight most highly expressed miRNAs from salivary glands potentially target human blood-expressed genes, suggesting that R. prolixus may modulate the host’s gene expression at the bite site. This study provides the first characterization of miRNAs in a Triatominae species, shedding light on the role of these crucial regulatory molecules.

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Trophic microRNA: Post‐transcriptional regulation of target genes and larval development impairment in Plutella xylostella upon precursor and mature microRNA ingestion

Bardapurkar,

Binayak,

Pandit

2024

Insect Molecular Biology

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MicroRNAs (miRNAs) are post‐transcriptional gene regulators. In the miRNA pathway's cytoplasmic part, the miRNA is processed from a hairpin‐structured precursor to a double‐stranded (ds) mature RNA and ultimately to a single‐stranded mature miRNA. In insects, ingesting these two ds forms can regulate the target gene expression; this inspired the trophic miRNA's use as a functional genomics and pest management tool. However, systematic studies enabling comparisons of pre‐ and mature forms, dosages, administration times and instar‐wise effects on target transcripts and phenotypes, which can help develop a miRNA administration method, are unavailable due to the different focuses of the previous investigations. We investigated the impact of trophically delivered Px‐let‐7 miRNA on the lepidopteran pest Plutella xylostella, to compare the efficacies of its pre‐ and ds‐mature forms. Continuous feeding on the miRNA‐supplemented diet suppressed expressions of FTZ‐F1 and E74, the target ecdysone pathway genes. Both the pre‐let‐7 and mature let‐7 miRNA forms similarly downregulated the target transcripts in all four larval instars. Pre‐let‐7 and let‐7 ingestions decreased larval mass and instar duration and increased mortality in all instars, exhibiting adverse effects on larval growth and development. miRNA processing Dicer‐1 and AGO‐1's upregulations upon miRNA ingestion denoted the systemic miRNA spread in larval tissues. The scrambled sequence controls did not affect the target transcripts, suggesting the sequence‐specific targeting by the mature miRNA and hairpin cassette's non‐involvement in the target downregulation. This work provides a framework for miRNA and target gene function analyses and potentiates the trophic miRNA's utility in pest management.

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Identification and profiling of stable microRNAs in hemolymph of young and old Locusta migratoria fifth instars

Cited by 3 publications

References 106 publications

Evaluation of chemosterility effect of different insect growth regulators on Bactrocera zonata population

Evaluation of chemosterility effect of different insect growth regulators on Bactrocera zonata population

Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease

Trophic microRNA: Post‐transcriptional regulation of target genes and larval development impairment in Plutella xylostella upon precursor and mature microRNA ingestion

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