Identification and quantification of components in extracts of Uncaria tomentosa by HPLC‐ES/MS

Abstract

The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as 'uña de gato', have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC-ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on flow injection ES/MS, and a more complete analytical technique using HPLC-MS.

Cat's claw oxindole alkaloid isomerization induced by common extraction methods

“…The analysis of POA and TOA transitions at m/z 369.3 → 337.1, and 385.4 → 353.4, respectively (Figure 1Sc and 1Sd, supplementary material), also allowed the recognition of each alkaloid class. 19 The identity of mitraphylline (1), isomitraphylline (2), pteropodine (5), and isopteropodine (6) was confirmed through co-elution with reference substances. In addition, speciophylline (3), uncarine F (4), rhyncophylline (7), and isorhyncophylline (8) followed a similar elution order to that formerly reported by other authors.…”

“…[20][21][22] Neither corynoxeine (9) nor isocorynoxeine (10) were detectable by HPLC-PDA or HPLC-MS/ MS, probably due to their low content in cat's claw bark. 19 …”

Macrophage colony stimulating factor: Not just for macrophages anymore! A gateway into complex biologies

“…This oxindole ring structure is naturally found within a herbal medicine (Cat's Claw, Una de Gato, Uncaria tomentosa) which has long been used by South Americans to treat various diseases, including cancer [190][191][192][193][194]. In Cat's Claw there are multiple derivatives of the oxindole ring (isopteropodine, pteropodine, isomitraphylline, rhynchophylliine, mitraphylline, hirsutine, gambirine, isorynchophylline and uncarine F) that are found in bark and roots of the plant source of these compounds.…”

Controlling concentration of bioactive components in cat's claw based products with a hybrid separation process

“…al. [27]. Prior to the chemical analysis, alkaloids were isolated by partition with chloroform and Na2CO3 solution.…”