Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant Huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection

Horvath, Christopher R.; Martos, Perry; Saxena, Praveen K.

doi:10.1016/j.chroma.2004.11.030

Cited by 117 publications

(69 citation statements)

References 25 publications

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“…Using LC-DAD, we observed the presence of all 10 analytes in S. baicalensis and S. tomentosa (Table 1). Roots of S. baicalensis contained high levels of baicalin (25.41 ± 0.10 lg mg -1 ) relative to stems (7.66 ± 0.01 lg mg -1 ) and leaves (4.03 ± 0.10 lg mg -1 ) ( Table 1), in agreement with previous studies showing that baicalin is predominantly found in roots of S. baicalensis (Horvath et al 2005;Makino et al 2008;Parajuli et al 2009). Direct comparisons of the levels quantified in this study with previous reports are difficult due to differences in the manner in which the levels were presented.…”

Section: Resultssupporting

confidence: 91%

Comparative analysis of bioactive phytochemicals from Scutellaria baicalensis, Scutellaria lateriflora, Scutellaria racemosa, Scutellaria tomentosa and Scutellaria wrightii by LC-DAD-MS

Islam

Downey

2010

Metabolomics

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Scutellaria is a geographically widespread and diverse genus of the Lamiaceae family of herbaceous plants commonly known as skullcaps. Scutellaria is used widely as an ethnobotanical herb for the treatment of various ailments ranging from cancers, cirrhosis, jaundice, hepatitis, anxiety and nervous disorders. We used (1) reverse-phase liquid chromatography coupled to a diode array detector (LC-DAD), and (2) multiple reaction monitoring (MRM) using mass spectrometry (LC-MS/MS) to quantify the levels of acteoside, scutellarin, scetellarein, baicalin, baicalein, wogonin, wogonoside, apigenin, chrysin, and oroxylin A in aqueous methanolic extracts of roots, shoots and leaves of S. baicalensis, S. lateriflora, S. racemosa, S. tomentosa and S. wrightii. Our results indicate that both methods (LC-DAD and LC-MS/MS) were robust for the detection of the 10 analytes from Scutellaria extracts although greater sensitivities were achieved using LC-MS/ MS in MRM mode. MRM enabled the detection of low levels of analytes which were otherwise undetected using LC-DAD. The baicalin content of S. wrightii roots were 5-fold higher than the commonly used S. baicalensis. Additionally, we also showed that leaves of both S. wrightii and S. tomentosa are good sources of scutellarin compared to S. baicalensis. Our data clearly demonstrated that previously uncharacterized species, S. wrightii and S. tomentosa are both good sources of flavonoids, particularly scutellarin, baicalin, wogonin and baicalein.

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Section: Resultssupporting

confidence: 91%

Comparative analysis of bioactive phytochemicals from Scutellaria baicalensis, Scutellaria lateriflora, Scutellaria racemosa, Scutellaria tomentosa and Scutellaria wrightii by LC-DAD-MS

Islam

Downey

2010

Metabolomics

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“…The storage stability of immobilized lipase was significantly better than the free form at 20°C within 20 days, and it retained about 55 % of the initial activity after being used for six times [35]. [36][37][38], compounds 1, 2, and 3 are identified as baicalin, wogonin, and oroxylin A, respectively.…”

Section: Synthesis and Characterization Of Lmnpsmentioning

confidence: 99%

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

et al. 2016

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Scutellaria baicalensis is a traditional Chinese medicinal plant possessing a wide variety of biological activities. In this work, lipase immobilized on magnetic nanoparticles (LMNPs) was used as solid phase extract absorbent for screening of lipase inhibitors from this plant. Three flavonoids were found to bind to LMNPs and were identified as baicalin, wogonin, and oroxylin A by liquid chromatography-mass spectrometry (HPLC-MS). Their IC 50 values were determined to be 229.22 ± 12.67, 153.71 ± 9.21, and 56.07 ± 4.90 μM, respectively. Fluorescence spectroscopy and molecular docking were used to probe the interactions between these flavonoids and lipase. All the flavonoids quenched the fluorescence of lipase statically by forming new complexes, implying their affinities with the enzyme. The thermodynamic analysis suggested that van der Waals force and hydrogen bond were the main forces between wogonin and lipase, while hydrophobic force was the main force for the other two flavonoids. The results from a molecular docking study further revealed that all of them could insert into the pocket of lipase binding to a couple of amino acid residues.

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“…Scutelaria baicalensis is an important medicinal herb with antibacterial and antiviral effects (Kim et al 1999) that has been used successfully to treat various human ailments, including fever, ulcer, cancer and inflammation (Horvath et al 2005). The extract of Scutellaria root has antimicrobial activity and is effective against many types of bacteria, such as Streptococcus, Mycobacterium and Pseudomonas (Tan and Vanitha 2004).…”

Section: Scutelaria Baicalensismentioning

confidence: 99%

The use of immunostimulating herbs in fish. An overview of research

Jeney

Yin

Ardó

et al. 2009

Fish Physiol Biochem

212

109

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The use of immunostimulants as an alternative to the drugs, chemicals and antibiotics currently being used to control fish diseases in fish culture is attracting the attention of many researchers. In this context, many have focused on the use of medicinal plant products as potential therapeutic measures for modulating the immune response and, specifically, on the use of herbs to prevent and control fish diseases. Medicinal plants (plant remedies) are a deeply rooted component of the cultural heritage of many people from diverse cultures and countries and are, as such, closely linked to the maintenance of good health. The aim of this paper is to review research currently being carried out on the herbs and herbal extracts that have been shown to modulate the immune system of fish. Special attention is given to the use of Chinese and Indian herbs.

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Identification and quantification of eight flavones in root and shoot tissues of the medicinal plant Huang-qin (Scutellaria baicalensis Georgi) using high-performance liquid chromatography with diode array and mass spectrometric detection

Cited by 117 publications

References 25 publications

Comparative analysis of bioactive phytochemicals from Scutellaria baicalensis, Scutellaria lateriflora, Scutellaria racemosa, Scutellaria tomentosa and Scutellaria wrightii by LC-DAD-MS

Comparative analysis of bioactive phytochemicals from Scutellaria baicalensis, Scutellaria lateriflora, Scutellaria racemosa, Scutellaria tomentosa and Scutellaria wrightii by LC-DAD-MS

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

The use of immunostimulating herbs in fish. An overview of research

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