Identification and quantification of monomers released from dental composites using HPLC

Archegas, Lucí Regina Panka; Rached, Rodrigo Nunes; Ignácio, Sérgio Aparecido; Vasconcelos, Eliane Carvalho de; Ramos, Débora Toledo; Souza, E. M.

doi:10.1590/s1516-89132009000400009

Cited by 13 publications

(15 citation statements)

References 23 publications

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“…Thus, our aim was to reach LLOQs as low as possible and consequently sufficient sensitivity. In this sense, for BisEMA, BisGMA, BPA, CQ, HEMA, TEGDMA, and UDMA, the LLOQs reached using our method are lower or similar to the ones reported in literature . To the best of our knowledge, no data on the LLOQs for the other target compounds was available for comparison.…”

Section: Resultssupporting

confidence: 71%

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

et al. 2017

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Resin-based dental materials have raised debates concerning their safety and biocompatibility, resulting in a growing necessity of profound knowledge on the quantity of released compounds into the oral cavity. In this context, the aim of this study was to develop a comprehensive and reliable procedure based on liquid chromatography with mass spectrometry for the simultaneous analysis of various leached compounds (including bisphenol A based compounds) in samples from in vitro experiments. Different experiments were performed to determine the optimal analytical parameters, comprising mass spectrometry parameters, chromatographic separation conditions, and sample preparation. Four internal standards were used as follows: deuterated diethyl phthalate and bisphenol A (commercially available), and deuterated analogues of triethylene glycol dimethacrylate and urethane dimethacrylate (custom-made). The optimized method was validated for linearity of the calibration curves and the associated correlation coefficient, lower limit of quantification, higher limit of quantification, and intra- and interassay accuracy and precision. Additionally, the developed liquid chromatography with tandem mass spectrometry method was applied to the analysis of leaching compounds from four resin-based dental materials. The results indicated that this method is suitable for the analysis of different target compounds leaching from dental materials. This method might serve as a valuable basis for quick and accurate quantification of leached compounds from resin-based dental materials in biological samples.

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Section: Resultssupporting

confidence: 71%

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

et al. 2017

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“…In the time interval of 7 days, the orthodontic cements RMO and RS presented a larger quantity of MOs, when compared with the other groups. The higher level of intensity of these cells may be related to the presence and release (Ak, Alpoz, Bayraktar, & Ertugrul, ; Archegas et al, ) of the monomer BisGMA in these materials for this period of time. There are indications that this monomer may be capable of inducing a greater extent of recruitment and activation of MOs (Yamanaka et al, ).…”

Section: Discussionmentioning

confidence: 99%

Morphological and immunohistochemical analysis of the biocompatibility of resin‐modified cements

Mesquita

Santos

Godoy

et al. 2016

Microscopy Res & Technique

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The aim of this double-blind randomized study was to evaluate the biocompatibility of resin-modified glass ionomer cements (RMGIC) by means of morphological and immunohistochemical analyses. RMGICs were selected and divided into four groups: Group CK (Crosslink Orthodontic Band Cement); Group RS (Resilience Light Cure Band Cement) Group RMO (RMO Band Cement), Group TP (Transbond Plus Light Cure Band), and Group C (Control-polyethylene). The materials were implanted in rat subcutaneous tissues, randomly selected for this study. After time intervals of 7, 15, and 30 days the tissues were submitted to morphological analysis. In immunohistochemical analysis, the immuno-marking of antibody CD68 was evaluated. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn tests (p < .05). In the morphological analysis after 7 days, Groups RS, RMO and TP showed more intense inflammatory infiltrate (p = .004) and only Group RMO presented greater intensity of multinucleated giant cells (p = .027). In the immunohistochemical analysis, Groups RMO and RS were observed to present a larger quantity of CD68+ (p = .004) in the time interval of 7 days and only Group RMO presented statistically significant difference for this parameter after 15 days (p = .026). In the time interval of 30 days, Group RMO presented the largest quantity of multinucleated giant cells (p < .004). The RMGICS Crosslink and Transbond Plus provided significantly better tissue biocompatibility than the Resilience and RMO Cements.

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“…Their negative effects include irritation of the soft tissues such as mucosal or pulpal tissues (11), stimulation of bacterial growth, allergic and cytotoxic reactions, and genotoxic, mutagenic, and oestrogenic effects (12,13).…”

Section: Introductionmentioning

confidence: 99%

“…The leaching of residual monomers is also believed to contribute to the degradation of a resin or resin composites (11). The amount of leached monomers is thus, associated with the DC (at least to some extent), but not all residual monomers can be eluted out.…”

Section: Introductionmentioning

confidence: 99%

Degree of conversion and leached monomers of urethane dimethacrylate-hydroxypropyl methacrylate-based dental resin systems

Zhang

Puska

Botelho

et al. 2016

Journal of Oral Science

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Identification and quantification of monomers released from dental composites using HPLC

Abstract: The aim of this study was to detect and quantify the main residual monomers released from composites, using high performance liquid chromatography (HPLC). Discs were made with dental composites (Herculite XRV, Tetric Ceram and Filtek Z250)

Cited by 13 publications

References 23 publications

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

Morphological and immunohistochemical analysis of the biocompatibility of resin‐modified cements

Degree of conversion and leached monomers of urethane dimethacrylate-hydroxypropyl methacrylate-based dental resin systems

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