IDENTIFICATION AND QUANTIFICATION OF POLYPHENOLS IN <i>RHIZOMA SMILACIS CHINAE</i> BY HPLC/DAD/ESI-MS/MS

Guo, Yu-Xian; Shangguan, Xinchen; Zheng, Guodong; Wang, Wenjun

doi:10.1080/10826076.2012.720326

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Peaks 16 to 19 were identified as neoastilbin, isoastilbin, neoisoastilbin, and astilbin ( Figure 3). (C 21 H 21 O 11 -) (Zhang et al, 2013), respectively, using spiking experiments with standard compounds. The structural identification of isoastilbin was confirmed by NMR analysis ( Supplementary Material, Figures S3-S9).…”

Section: Flavanonesmentioning

confidence: 99%

Metabolomic Analysis, Fast Isolation of Phenolic Compounds, and Evaluation of Biological Activities of the Bark From Weinmannia trichosperma Cav. (Cunoniaceae)

Barrientos

Fernández-Galleguillos

Pastene

et al. 2020

Front. Pharmacol.

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Weinmannia trichosperma Cav. (Cunoniaceae) (local name, tineo; Mapuche names, madeń, mëdehue) is an endemic species of Chile and Argentina used in Mapuche traditional medicine in the treatment of chronic diarrhea, inflammation, and wound healing. This study focused on the isolation, analysis, and characterization of the biological activity of compounds and bark extracts from this plant for the first time. The infusion and tincture of the bark were characterized regarding antioxidant and important enzyme inhibitory activities, phenolics, and flavonoids content and UHPLC-ESI-OT-MS metabolite profiling. Twenty-five metabolites were detected in the medicinal infusion of W. trichosperma, three flavonols were isolated: isoastilbin, neoisoastilbin, and neoastilbin ((2R,3S)-, (2S,3R)-, and (2S,3S)-dihydroquercetin 3-O-alpha-L-rhamnoside) by countercurrent chromatography, and the isomers were quantified in the bark using a validated analytical HPLC methodology. The antioxidant properties were measured by ABTS, DPPH, FRAP, ORAC, and TEAC methods. The infusion displayed a strong DPPH and ABTS scavenging activity (IC 50 = 20.58 and 3.070 µg ml −1 , respectively) while a moderated effect was observed in the FRAP, ORAC, and ABTS assays. The infusion showed a content of phenolic and flavonoid compounds of 442.1 mg GAE g −1 and 15.54 mg QE g −1 , respectively. Furthermore, the infusion showed a good and promissory inhibitory activity (33.80%, 33.12%, and 82.86% for AChE, BuChE, and 5-hLOX, respectively) and isoastilbin (51.70%, 50.10%, and 34.29-80.71% for AChE, BuChE, and 5-hLOX, respectively). The biomolecules identified in this study support the traditional uses of this bark and the potential industrial interest from this Valdivian plant species.

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Section: Flavanonesmentioning

confidence: 99%

Metabolomic Analysis, Fast Isolation of Phenolic Compounds, and Evaluation of Biological Activities of the Bark From Weinmannia trichosperma Cav. (Cunoniaceae)

Barrientos

Fernández-Galleguillos

Pastene

et al. 2020

Front. Pharmacol.

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show abstract

“…[24] In another study, method by UPLC-MS with sunfire TM C 18 column (2.1mm x 50mm, 3.5 µm) and electrospray ionization technique using the mobile phase of acetonitrile and 0.3 percent acetic acid by eluting in gradient mode from 10 percent acetonitrile followed by 35 percent acetonitrile and then 10 percent acetonitrile for quantification in rat plasma has been reported. [25]…”

Section: Introductionmentioning

confidence: 99%

Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC

Subramanian¹,

Shakila²

2021

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Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C 18 column (shim-pack) of size 150 mm x 4.6 mm; 5µ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at R t 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 µg/ml, calibration curves were produced with a linear relationship R 2 > 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 µg/ml; quantification limits were 0.473 and 0.234 µg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RP-HPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides.

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The natural source, physicochemical properties, biological activities and metabolism of astilbin

Yang

2022

Critical Reviews in Food Science and Nutrition

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IDENTIFICATION AND QUANTIFICATION OF POLYPHENOLS IN RHIZOMA SMILACIS CHINAE BY HPLC/DAD/ESI-MS/MS

Cited by 5 publications

References 16 publications

Metabolomic Analysis, Fast Isolation of Phenolic Compounds, and Evaluation of Biological Activities of the Bark From Weinmannia trichosperma Cav. (Cunoniaceae)

Metabolomic Analysis, Fast Isolation of Phenolic Compounds, and Evaluation of Biological Activities of the Bark From Weinmannia trichosperma Cav. (Cunoniaceae)

Isolation, Simultaneous Quantification of Taxifolin and Taxifolin-3-O-rhamnoside and Validation by RPHPLC

The natural source, physicochemical properties, biological activities and metabolism of astilbin

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