Identification and quantification of the glucose degradation product glucosone in peritoneal dialysis fluids by HPLC/DAD/MSMS

Mittelmaier, Stefan; Fünfrocken, Michael; Fenn, Dominik; Fichert, Thomas; Pischetsrieder, Monika

doi:10.1016/j.jchromb.2010.02.004

Cited by 37 publications

(44 citation statements)

References 32 publications

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“…Optimization of Incubation Conditions-HEPES was used as a buffer for derivatization because it was recently shown by Mittelmaier et al (25) to be a good medium for dicarbonyl derivatization by OPD. DHA (1), being the least reactive of the tested dicarbonyls, was used for optimization of incubation conditions.…”

Section: Resultsmentioning

confidence: 99%

Vitamin C Degradation Products and Pathways in the Human Lens

Nemet

Monnier

2011

Journal of Biological Chemistry

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Vitamin C and its degradation products participate in chemical modifications of proteins in vivo through non-enzymatic glycation (Maillard reaction) and formation of different products called advanced glycation end products. Vitamin C levels are particularly high in selected tissues, such as lens, brain and adrenal gland, and its degradation products can inflict substantial protein damage via formation of advanced glycation end products. However, the pathways of in vivo vitamin C degradation are poorly understood. Here we have determined the levels of vitamin C oxidation and degradation products dehydroascorbic acid, 2,3-diketogulonic acid, 3-deoxythreosone, xylosone, and threosone in the human lens using o-phenylenediamine to trap both free and protein-bound adducts. In the protein-free fraction and water-soluble proteins (WSP), all five listed degradation products were identified. Dehydroascorbic acid, 2,3-diketogulonic acid, and 3-deoxythreosone were the major products in the protein-free fraction, whereas in the WSP, 3-deoxythreosone was the most abundant measured dicarbonyl. In addition, 3-deoxythreosone in WSP showed positive linear correlation with age (p < 0.05). In waterinsoluble proteins, only 3-deoxythreosone and threosone were detected, whereby the level of 3-deoxythreosone was ϳ20 times higher than the level of threosone. The identification of 3-deoxythreosone as the major degradation product bound to human lens proteins provides in vivo evidence for the non-oxidative pathway of dehydroascorbate degradation into erythrulose as a major pathway for vitamin C degradation in vivo.

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Section: Resultsmentioning

confidence: 99%

Vitamin C Degradation Products and Pathways in the Human Lens

Nemet

Monnier

2011

Journal of Biological Chemistry

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“…Therefore, the simultaneous separation of the dicarbonyl intermediates of AGEs by reversed-phase chromatography using an ODS column is very difficult due to the adsorption on the stationary phase compounds. In a conventional study, the 3-deoxyglucosone in rat and human plasma samples were successfully determined by HPLC separation and FL, diode array detector (DAD) [15,20]. However, these derivatization reagents have a long derivatization time, and many admixture peaks.…”

Section: Determination Of the Intermediates Of Ages In Human Fingernailmentioning

confidence: 99%

Development of the High Sensitive Separation Analysis Method of Metabolites in Human Nail and its Application to the Diagnosis of Chronic Disease

Min

2014

Chromatography

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This review summarizes the possibility to diagnosing the chronic diseases and application of determination of metabolites of the noninvasive human nails. A target derivatization UPLC-ESI-MS method is a powerful tool for the analysis of bioactive compounds with high sensitivity and selectivity. We developed target derivatization UPLC-ESI-MS methods to analyze the biological trace components in human fingernail. Moreover, we have used these methods to detect the metabolites in human fingernails, such as DL-amino acids, polyamines and Advanced Glycation End products (AGEs). Through detecting the biological trace components in the real samples from human, we tested the possibility to use these new methods to diagnosing the chronic diseases, i.e., diabetes, lung cancer. Some applications utilizing these target derivatization methods for the analyses determination of metabolites in the human fingernails are also described in this paper.

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“…-Oxoaldehydes and type 2 diabetes Numerous techniques have been described for the quantification of the reactive -oxoaldehydes glyoxal (GO), methylglyoxal (MGO) and 3-deoxyglucosone (3-DG) including reversed phase liquid chromatography with fluorescence or UV detection [66][67][68][69][70][71] or liquid chromatography with mass spectrometry detection [72][73][74][75][76] . However, these techniques have several shortcomings such as large sample volume 67,69,72,73 , elaborate liquid-liquid extraction (LLE) or solid phase extraction (SPE) steps 66,67,[72][73][74][75] and long run-times 66,68,71 .…”

Section: Discussionmentioning

confidence: 99%

“…However, these techniques have several shortcomings such as large sample volume 67,69,72,73 , elaborate liquid-liquid extraction (LLE) or solid phase extraction (SPE) steps 66,67,[72][73][74][75] and long run-times 66,68,71 .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, interference of other fluorophores, e.g. nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), porphyrins, dityrosine and N'-formylkynurenine [68][69][70] , or changes in the absorption of ultraviolet (UV) excitation light due to hemodynamic and oximetric effects 71 may influence the relationship between (auto)fluorescence and AGE. Therefore, the interpretation of SAF data, as an estimate of AGEs, and the relationship with disease should be interpreted with care.…”

Section: The Early Beginning Of Age Analysis: Fluorescence Detectionmentioning

Identification and quantification of the glucose degradation product glucosone in peritoneal dialysis fluids by HPLC/DAD/MSMS

Cited by 37 publications

References 32 publications

Vitamin C Degradation Products and Pathways in the Human Lens

Vitamin C Degradation Products and Pathways in the Human Lens

Development of the High Sensitive Separation Analysis Method of Metabolites in Human Nail and its Application to the Diagnosis of Chronic Disease

The analysis of advanced glycation endproducts

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