Identification and Screening of Citrus Vein Phloem Degeneration (CVPD) on Brastagi Citrus Variety Brastepu (Citrus nobilis Brastepu) in North Sumatra Indonesia

Nurwahyuni, Isnaini; Napitupulu, Justin A.; Rosmayati, Rosmayati; Harahap, Urip

doi:10.5539/jas.v7n4p30

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…The research was conducted at the Laboratory of Plant Physiology and Plant Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, and in the forest area at Pakfak Bharat Regency, North Sumatra Indonesia. The research procedure consisted of field surveys, taking plant material from the field as explants, and micropropagation [14] [15]. Each part of this procedure is briefly described below.…”

Section: Methodsmentioning

confidence: 99%

“…Styrax benzoin plant has high economic potential because it produces sap containing bioactive compounds as raw materials for medicine and cosmetics. However, the cultivation of frankincense has not been carried out massively because of the difficulty in providing good quality seeds [13]. Plants that grow in forest areas are trees that grow naturally from seeds.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Sumatran Frankincense (Styrax Benzoin) Forest Plants Producing Bioactive Raw Materials for Medicine

Nurwahyuni

Situmorang

Sinaga

2023

Advances in Science and Technology

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Preservation of tropical forest plants that have high economic potential, such as Sumatran benzoin (Styrax benzoin Dryander), is urgently carried out through the provision of quality seeds. The purpose of this study was to propagate the Sumatran Frankincense plant to produce good quality seeds to meet the needs of seeds for forest conservation purposes. The micropropagation technique was carried out using explants from selected parent plants of very good quality. Micropropagation through the use of explants from several parts of the plant aims to produce seeds with the same incense production quality as the parent plant. Explants were selected from the young leaves of the shoots. The experiment was conducted in a completely randomized design with two factors: concentrations of NAA (0-3 mg/L) and BAP (0-3 mg/L). The combination of NAA and BAP in culture media under optimal conditions proved to be effective in inducing callus and plantlet growth. The success of micropropagation has the potential to produce seeds of the same excellent quality as the parent plant in large quantities in a relatively short time. This technique is expected to be able to meet the conservation needs of people's forests and national forests, and in the long term will increase the production of frankincense as a non-timber forest commodity as raw material for medicine.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Micropropagation of Sumatran Frankincense (Styrax Benzoin) Forest Plants Producing Bioactive Raw Materials for Medicine

Nurwahyuni

Situmorang

Sinaga

2023

Advances in Science and Technology

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“…Jumlah tunas dan akar terbanyak dihasilkan pada perlakuan kombinasi 0,05 mg/L NAA dan 0,31 mg/L BAP. Penelitian Nurwahyuni (2013) mengenai perbanyakan Citrus nobilis Brastepu menggunakan eksplan pucuk diperoleh bahwa konsentrasi 0,5 mg/L BAP efektif dalam memulai pertumbuhan pucuk. Penelitian lain oleh Tilaar & Tulung (2013) tentang kultur pucuk juga telah dilakukan pada tanaman brokoli.…”

Section: Pendahuluanunclassified

In Vitro Shoot Culture Growth of Pontianak Orange (Citrus nobilis var. microcarpa) with the Addition of Naphthalene Acetic Acid and 6-Benzyl Amino Purine

Nissa,

Zakiah,

Turnip

2023

JBT

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Pontianak siam orange (Citrus nobilis var. microcarpa) is an important agricultural commodity unique to Sambas Regency, West Kalimantan. The demand for Pontianak siam oranges has been steadily increasing over the years, yet the production of Pontianak oranges has not been able to meet the domestic demand. The limited availability of superior seedlings is one of the reasons for this situation. Therefore, efforts are needed to fulfill the demand for Pontianak siam oranges by producing a large quantity of superior seedlings in a short period. One of the strategies that can be employed is in vitro propagation. This research aims to observe the growth of Pontianak Siamese orange shoot explants after administration of NAA (Naphthalene Acetic Acid) and BAP (6-Benzyl Amino Purine). This research was conducted from January to June 2023 at the Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The study employed a Completely Randomized Design (CRD) with two treatment factors namely NAA concentrations with 4 levels (0; 0.25; 0.5; 0.75 mg/L), and BAP concentrations with 5 levels (0; 0.5; 1; 1.5; 2 mg/L). Parameters observed were the emergence time of shoot, shoot number, height of shoot, and the number of leaves. The results showed that single NAA, single BAP, and combination treatment of NAA and BAP significantly affected all growth parameters. Treatment with the addition of 0.25 mg/L NAA and 1 mg/L BAP (A1B2) was the treatment with the fastest shoot emergence time (2.80 days after planting), the largest number of shoots (2.60 shoots)the tallest shoots (1.50 cm), , and the greatest number of leaves (2.60 leaves).

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“…The productivity of mulberry plants can be increased by expanding the area of mulberry planting to saline areas that were previously unusable. The area of saline land in Indonesia is estimated to be around 440.300 ha [5].…”

Section: Introductionmentioning

confidence: 99%

Effect of salinity on glandular trichomes density, phenolic and flavonoid contents of Morus spp. accessions leaves from Indonesia

Wulandari,

Sulistyaningsih,

Suprayogi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The density of glandular trichomes and metabolite compounds in plants can increase under salt stress. Information about the glandular trichomes density described tolerance to salt stress however, there is no information about it in mulberry. This study aimed to obtain accessions of mulberry that are tolerant to NaCl concentrations based on cystolith trichome density, glandular trichome density, and phenolic and flavonoid content. Mulberry stem cuttings were planted using a factorial randomized block design with 3 replications. The first treatment used was seven mulberry accessions, which come from 5 regions in Indonesia (Bali, Makassar, Pati, Situbondo, and Bogor), and the second was the NaCl concentration (0.0%, 0.2%, 0.3%, and 0.4%). The results showed that the highest cystolith trichomes density was obtained in M2 accession (32.5 cells/mm2 from the highest NaCl concentration). The highest density of the glandular trichomes was obtained in the M4 accession (50 cells/mm2 from the highest NaCl concentration). The highest phenolic and flavonoid contents were obtained in the M4 accession (154.6 mg GAE/g extract for 0.3% concentration of NaCl) and M2 accession (619.56 mg QE/g extract for the highest concentration of NaCl), respectively. The study concluded that the M2 (accession from Bogor) is tolerant to salt stress.

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Identification and Screening of Citrus Vein Phloem Degeneration (CVPD) on Brastagi Citrus Variety Brastepu (Citrus nobilis Brastepu) in North Sumatra Indonesia

Cited by 6 publications

References 25 publications

Micropropagation of Sumatran Frankincense (Styrax Benzoin) Forest Plants Producing Bioactive Raw Materials for Medicine

Micropropagation of Sumatran Frankincense (Styrax Benzoin) Forest Plants Producing Bioactive Raw Materials for Medicine

In Vitro Shoot Culture Growth of Pontianak Orange (Citrus nobilis var. microcarpa) with the Addition of Naphthalene Acetic Acid and 6-Benzyl Amino Purine

Effect of salinity on glandular trichomes density, phenolic and flavonoid contents of Morus spp. accessions leaves from Indonesia

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