Identification and sequence analyses of the granulin gene of Choristoneura fumiferana granulovirus

Abstract

The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined. The gene encodes a protein of 248 amino acids with a predicted Mr of 29.299 kDa. The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging from 76.7-80.5% for nucleotide sequences and 84.2-88.3% for amino acid sequences when compared to CfGV. The secondary structure of CfGV granulin protein, including the hydrophilic (polar) and hydrophobic (basic) regions, was predicted and found to be similar to other granulins. A very late baculovirus promoter motif, ATAAG, was found within the putative promoter region of the CfGV granulin gene.

Choristoneura fumiferana Granulovirus p74 Protein, a Highly Conserved Baculoviral Envelope Protein

“…In vivo production and purification of virus and DNA extraction Choristoneura fumiferana fourth-instar larvae were infected by ChfuGV using a virus-contaminated artificial diet (Forté et al, 1999). The virus isolation from infected larvae that was followed by the DNA extraction was carried out as previously described (Bah et al, 1997).…”

Identification and Characterization of a Conserved Baculoviral Structural Protein ODVP-6E/ODV-E56 from Choristoneura fumiferana Granulovirus

“…Next, the virus isolation from infected larvae by DNA extraction was carried out, as previously described (Bah et al, 1997).…”

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

“…The virus isolated from the infected larvae was carried out as previously described (Bah et al, 1997).…”