1989
DOI: 10.1128/jb.171.7.3840-3845.1989
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Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator

Abstract: The xyl operator of Bacillus subtilis W23 was identified by deletion analysis of the xyl regulatory region as a 25-base-pair (bp) sequence located 10 bp downstream from the xyl promoter. The outer 10 bp of the xyl operator exhibit perfect palindromic symmetry, while 5 central bp are nonpalindromic. It was demonstrated that the penultimate base pair near the end of this sequence is important for repressor binding. In both strains, expression of xylose-utilizing enzymes appears to be negatively regulated at the … Show more

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Cited by 74 publications
(39 citation statements)
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“…As described above, the upstream region influences promoter activity and heat shock induction. The sequence upstream of P xylA , which is necessary for the full activity of this promoter (25), had no positive effect on the nfrA promoter (Fig. 5).…”
Section: Resultsmentioning
confidence: 99%
“…As described above, the upstream region influences promoter activity and heat shock induction. The sequence upstream of P xylA , which is necessary for the full activity of this promoter (25), had no positive effect on the nfrA promoter (Fig. 5).…”
Section: Resultsmentioning
confidence: 99%
“…The deduced amino acid sequence of XylR is homologous to that of XylR of Bacillus subtilis (17), Bacillus megaterium (26), Bacillus licheniformis (30), and Staphylococcus xylosus (31). The repressor function of XylR has been unambiguously demonstrated in these organisms (15,17,29,32).…”
mentioning
confidence: 87%
“…Different cis-acting catabolic control sequences have been proposed for a number of operons (7,15,20,36). Catabolite repression of xyl in B. megaterium is more efficient than that in B. subtilis W23 (8,15,16), thus facilitating the study of effects of other carbon sources repressing less effectively than glucose. …”
mentioning
confidence: 99%