Identification and structural analysis of novel malathion-specific DNA aptameric sensors designed for food testing

Kadam, Ulhas Sopanrao; Trinh, Kien Hong; Kumar, Vikas; Lee, Keun Woo; Cho, Yuhan; Can, Mai-Huong Thi; Lee, Hyebi; Kim, Yujeong; Kim, Sundong; Kang, Jae-Hee; Kim, Jae‐Yean; Chung, Woo Sik; Hong, Jong Chan

doi:10.1016/j.biomaterials.2022.121617

Cited by 29 publications

(13 citation statements)

References 42 publications

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“…Interestingly, the G-quadruplex structure can be bound by some fluorescent dyes to produce strong fluorescence, such as Thioflavin T (ThT) ( Kadam et al, 2022 ; Trinh et al, 2022 ) and SGI ( Chen X. et al, 2018 ; Li, Tu & Luo., 2018). A simple SGI dye experiment was used to verify that Apt-M13 formed a G-quadruplex structure.…”

Section: Resultsmentioning

confidence: 99%

“…When the mass ratio of GO to aptamer was 200:1, the aptamer was adsorbed by GO which made the fluorescence quenched. Then, the affinity of the mutant aptamer Apt-M13 and the original aptamer sequence Interestingly, the G-quadruplex structure can be bound by some fluorescent dyes to produce strong fluorescence, such as Thioflavin T (ThT) (Kadam et al, 2022;Trinh et al, 2022) and SGI (Chen X. et al, 2018;Li, Tu & Luo., 2018). A simple SGI dye experiment was used to verify that Apt-M13 formed a G-quadruplex structure.…”

Section: Go-based Fluorescence Detection Of Aptamer Affinitymentioning

confidence: 99%

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Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Qin

Bubiajiaer

et al. 2022

Front. Bioeng. Biotechnol.

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Various biosensors based on aptamers are currently the most popular rapid detection approaches, but the performance of these sensors is closely related to the affinity of aptamers. In this work, a strategy for constructed high-affinity aptamer was proposed. By truncating the bases flanking the 59 nt dexamethasones (DEX) original aptamer sequence to improve the sensitivity of the aptamer to DEX, and then base mutation was introduced to further improve the sensitivity and selectivity of aptamers. Finally, the 33 nt aptamer Apt-M13 with G-quadruplex structures was obtained. The dissociation constant (Kd) was determined to be 200 nM by Graphene oxide (GO)-based fluorometry. As-prepared Apt-M13 was used for a label-free colorimetric aptamer sensor based on gold nanoparticles, the LOD was 3.2-fold lower than the original aptamer described in previous works. The anti-interference ability of DEX analogs is also further improved. It indicates that truncation technology effectively improves the specificity of the aptamer to DEX in this work, and the introduction of mutation further improves the affinity and selectivity of the aptamer to DEX. Therefore, the proposed aptamer optimization method is also expected to become a general strategy for various aptamer sequences.

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Section: Resultsmentioning

confidence: 99%

Section: Go-based Fluorescence Detection Of Aptamer Affinitymentioning

confidence: 99%

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Qin

Bubiajiaer

et al. 2022

Front. Bioeng. Biotechnol.

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“…In addition to high selectivity and sensitivity, triple–quadrupole mass spectrometers provide practical solutions for simultaneous determination of multiple pesticide residues [ 26 , 27 ]. For method-establishment of pesticides in group 1 using UPLC-MS/MS, the single standard solution of 1 mg L − 1 for each pesticide was directly infused into the ESI source at 10 µL min − 1 to acquire the parent ions, daughter ions, as well as the corresponding optimised cone voltage and collision energy (CE).…”

Section: Resultsmentioning

confidence: 99%

Method Validation for Multi-Pesticide Residue Determination in Chrysanthemum

Wang

et al. 2023

Molecules

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The chrysanthemum can be consumed in various forms, representing the “integration of medicine and food”. Quantitative analysis of multi-pesticide residues in chrysanthemum matrices is therefore crucial for both product-safety assurance and consumer-risk evaluation. In the present study, a simple and effective method was developed for simultaneously detecting 15 pesticides frequently used in chrysanthemum cultivation in three matrices, including fresh flowers, dry chrysanthemum tea, and infusions. The calibration curves for the pesticides were linear in the 0.01–1 mg kg−1 range, with correlation coefficients greater than 0.99. The limits of quantification (LOQs) for fresh flowers, dry chrysanthemum tea, and infusions were 0.01–0.05 mg kg−1, 0.05 mg kg−1, and 0.001–0.005 mg L−1, respectively. In all selected matrices, satisfactory accuracy and precision were achieved, with recoveries ranging from 75.7 to 118.2% and relative standard deviations (RSDs) less than 20%. The validated method was then used to routinely monitor pesticide residues in 50 commercial chrysanthemum-tea samples. As a result, 56% of samples were detected with 5–13 pesticides. This research presents a method for the efficient analysis of multi-pesticide residues in chrysanthemum matrices.

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“…These techniques guarantee that they can rapidly, accurately, and cost-effectively detect biologically related compounds through biorecognition and signal transduction [ 16 , 17 , 18 ]. A biosensor is an analytical tool that combines a biological component with a physicochemical detection transducer for detecting specific analytes, such as DNA and proteins [ 19 , 20 , 21 , 22 ]. Moreover, electrical and optical signals coupled with specialized biological responses involving isolated enzymes, tissues, organelles, or whole cells are used to detect chemical compounds or whole cells [ 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability

Koo

Kim

et al. 2022

Biosensors

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Redox reactions in live cells are generated by involving various redox biomolecules for maintaining cell viability and functions. These qualities have been exploited in the development of clinical monitoring, diagnostic approaches, and numerous types of biosensors. Particularly, electrochemical biosensor-based live-cell detection technologies, such as electric cell–substrate impedance (ECIS), field-effect transistors (FETs), and potentiometric-based biosensors, are used for the electrochemical-based sensing of extracellular changes, genetic alterations, and redox reactions. In addition to the electrochemical biosensors for live-cell detection, cancer and stem cells may be immobilized on an electrode surface and evaluated electrochemically. Various nanomaterials and cell-friendly ligands are used to enhance the sensitivity of electrochemical biosensors. Here, we discuss recent advances in the use of electrochemical sensors for determining cell viability and function, which are essential for the practical application of these sensors as tools for pharmaceutical analysis and toxicity testing. We believe that this review will motivate researchers to enhance their efforts devoted to accelerating the development of electrochemical biosensors for future applications in the pharmaceutical industry and stem cell therapeutics.

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Identification and structural analysis of novel malathion-specific DNA aptameric sensors designed for food testing

Cited by 29 publications

References 42 publications

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Method Validation for Multi-Pesticide Residue Determination in Chrysanthemum

Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability

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