2018
DOI: 10.3389/fcimb.2018.00152
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Identification and Validation of an Antivirulence Agent Targeting HlyU-Regulated Virulence in Vibrio vulnificus

Abstract: Antimicrobial resistance (AMR) in pathogens is the result of indiscriminate use of antibiotics and consequent metabolic/genetic modulation to evolve survival strategies and clonal-selection in AMR strains. As an alternative to antibiotic treatment, antivirulence strategies are being developed, not only to combat bacterial pathogenesis, but also to avoid emerging antibiotic resistance. Vibrio vulnificus is a foodborne pathogen that causes gastroenteritis, necrotizing wound infections, and sepsis with a high rat… Show more

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Cited by 23 publications
(23 citation statements)
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“…The EMSA results showed the precise targeting of the known master virulence factor, HlyU ( Figure 4 A–C). We established a G. mellonella (wax-worm) infection model for studying V. vulnificus pathogenesis in an earlier study [ 12 ]. In this model, 2′,4′-DHC protected ~50% wax-worm larvae from V. vulnificus infection ( Figure 4 D,D’).…”
Section: Discussionmentioning
confidence: 99%
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“…The EMSA results showed the precise targeting of the known master virulence factor, HlyU ( Figure 4 A–C). We established a G. mellonella (wax-worm) infection model for studying V. vulnificus pathogenesis in an earlier study [ 12 ]. In this model, 2′,4′-DHC protected ~50% wax-worm larvae from V. vulnificus infection ( Figure 4 D,D’).…”
Section: Discussionmentioning
confidence: 99%
“…All recombinant DNA techniques were performed using various molecular biology kits for plasmid and genomic DNA isolation (Intron Biotech, Seongnam, Korea), and gel purification of DNA (Cosmogenetech LaboPass, Gel extraction kit, Seoul, Korea) as per the manufacturers’ protocols. An expand long PCR amplification kit (Qiagen, Hilden, Germany) was used to amplify the long DNA cassette (P rtxA 1 :: luxCDABE_cm r , 7.997 kb) [ 12 ] harboring the P rtxA1 promoter tagged with luxCDABE genes and chloramphenicol acetyltransferase gene using specific primers ( Table S1 ). The ~8 kb long region was flanked with a λ-red recombinase site for the genomic integration as described earlier [ 28 ].…”
Section: Methodsmentioning
confidence: 99%
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