Identification and Validation of Molecular Markers Associated with Pachymetra Root Rot and Brown Rust Resistance in Sugarcane Using Map- and Association-based Approaches

McIntyre, C. Lynne; Whan, Vicki; Croft, BJ; Magarey, R. C.; Smith, G. R.

doi:10.1007/s11032-005-7492-5

Cited by 31 publications

(19 citation statements)

References 29 publications

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“…QTL validation for different rust diseases caused by Puccinia sp. has been carried out in wheat (Khlestkina et al 2007;Schnurbusch et al 2004), barley (Castro et al 2003), and sugar cane (McIntyre et al 2005) to name a few. In all cases a major locus conferring resistance to the rust disease was found and its presence validated across unrelated pedigrees.…”

Section: Discussionmentioning

confidence: 99%

Positioning of the major locus for Puccinia psidii rust resistance (Ppr1) on the Eucalyptus reference map and its validation across unrelated pedigrees

Mamani

Bueno

Faria

et al. 2010

Tree Genetics & Genomes

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In this report the major locus for Puccinia psidii rust resistance, Ppr1, was positioned on the reference genetic map for Eucalyptus. Additionally, its position was validated by association genetics in a related and two unrelated pedigrees involving different Eucalyptus grandis resistant trees crossed to individuals of two other species, Eucalyptus tereticornis and Eucalyptus camaldulensis. These results are consistent with the hypothesis that Ppr1 controls a large proportion of the variation for rust resistance, strengthening its role as a major locus in Eucalyptus and providing its unequivocal genomic position on linkage group 3. A localized map with 19 microsatellite loci was built around Ppr1. Multiallelic profiles were observed at several mapped microsatellites suggesting recent tandem duplications in the genomic landscape surrounding Ppr1. Markers EMBRA125 and EMBRA1071 flank Ppr1 at 9.5% and 7% recombination, respectively, and were found to be in linkage equilibrium in a E. grandis breeding population, consistent with the expectations in outcrossed Eucalyptus. Their potential use for MAS will specifically be directed to identifying resistant offspring of P. psidii resistant parent trees that are heterozygous at Ppr1. In these circumstances, a significant amount of LD is expected to occur between specific alleles at flanking microsatellites and the resistance allele at Ppr1. Moreover, the positional information of Ppr1 paves the way for prospective undertakings in this genomic region with the upcoming availability of a draft genome for E. grandis.

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Section: Discussionmentioning

confidence: 99%

Positioning of the major locus for Puccinia psidii rust resistance (Ppr1) on the Eucalyptus reference map and its validation across unrelated pedigrees

Mamani

Bueno

Faria

et al. 2010

Tree Genetics & Genomes

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“…The first QTL mapping study in sugarcane was performed by Sills et al (1995). Other works studies making important contributions to QTL mapping include Ming et al (2001Ming et al ( , 2002a, Hoarau et al (2002), McIntyre et al (2005), da Silva and Bressiani (2005), Reffay et al (2005), Aitken et al (2006Aitken et al ( , 2008, Piperidis et al (2008), Pastina et al (2012), and Shing et al (2013). Specifically, Pastina et al (2012) proposed a mixed model for QTL mapping in sugarcane in order to include complex G 9 E interactions.…”

Section: Introductionmentioning

confidence: 99%

QTL mapping including codominant SNP markers with ploidy level information in a sugarcane progeny

et al. 2016

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Quantitative trait locus (QTL) mapping contributes to sugarcane (Saccharum spp.) breeding programs by providing information about the genetic effects, positioning and number of QTLs. Combined with marker-assisted selection, it can help breeders reduce the time required to develop new sugarcane varieties. We performed a QTL mapping study for important agronomic traits in sugarcane using the composite interval mapping method for outcrossed species. A new approach allowing the 1:2:1 segregation ratio and different ploidy levels for SNP markers was used to construct an integrated genetic linkage map that also includes AFLP and SSR markers. Were used 688 molecular markers with 1:1, 3:1 and 1:2:1 segregation ratios. A total of 187 individuals from a biparental cross (IACSP95-3018 and IACSP93-3046) were assayed across multiple harvests from two locations. The evaluated yield components included stalk diameter (SD), stalk weight (SW), stalk height (SH), fiber percentage (Fiber), sucrose content (Pol) and soluble solid content (Brix). The genetic linkage map covered 4512.6 cM and had 118 linkage groups corresponding to 16 putative homology groups. A total of 25 QTL were detected for SD (six QTL), SW (five QTL), SH (four QTL), Fiber (five QTL), Pol (two QTL) and Brix (three QTL). The percentage of phenotypic variation explained by each QTL ranged from 0.069 to 3.87 %, with a low individual effect because of the high ploidy level. The mapping model provided estimates of the segregation ratio of each E. A. Costa, C. O. Anoni, and M. C. Mancini have contributed equally to this work.Electronic supplementary material The online version of this article

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“…Há também mapas desenvolvidos a partir de populações derivadas ou envolvendo variedades comerciais ou clones elite como, por exemplo, S. officinarum "IJ76-514" (2n = 80) x "Q165" (2n ≈ 115) (AITKEN; JACKSON, McINTYRE, 2005; "Q117" x "MQ77-340" (REFFAY et al, 2005); "Q117" x Linhagem "74C42" (McINTYRE et al, 2005a); "SP80-180" x "SP80-4966" (GARCIA et al, 2006;OLIVEIRA et al, 2007); auto-fecundação da cultivar "R570" (2n = 107-115) (GRIVET et al, 1996;ASNAGHI et al, 2000;HOARAU et al, 2001;ROSSI et al, 2003);…”

Section: Aspectos Econômicos Do Agronegócio Sucroalcooleiro No Brasilunclassified

“…Utilizando esta abordagem em populações F 1 , diversos caracteres em cana-de-açúcar foram mapeados, incluindo caracteres relacionados a doenças, produção de massa e de açúcar (DAUGROIS et al, 1996;MUDGE et al, 1996;MING et al, 2001MING et al, , 2002bMING et al, , 2002cHOARAU et al, 2002;McINTYRE et al, 2005aMcINTYRE et al, , 2005bJACKSON;McINTYRE, 2006;RABOIN et al, 2006;AL-JANABI et. al., 2007;ALWALA et al, 2008;PIPERIDIS et al, 2008;PINTO et al, 2010).…”

Section: Aspectos Econômicos Do Agronegócio Sucroalcooleiro No Brasilunclassified

“…Esse fato tem sido confirmado em diversos estudos que identificaram grande número de alelos com pequenos efeitos sobre a variação fenotípica dos caracteres (SILLS et al, 1995;MING et al, 2001MING et al, , 2002bMING et al, , 2002cHOARAU et al, 2002;McINTYRE et al, 2005a;AL-JANABI et. al., 2007;PIPERIDIS et al, 2008;PINTO et al, 2010 O mapeamento por associação foi originalmente desenvolvido para mapear genes relacionados a doenças em humanos (KEREM et al, 1989).…”

Section: Aspectos Econômicos Do Agronegócio Sucroalcooleiro No Brasilunclassified

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Mapeamento genético de cana-de-açúcar (Saccharum spp.) por associação empregando marcadores SSR e AFLP

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Identification and Validation of Molecular Markers Associated with Pachymetra Root Rot and Brown Rust Resistance in Sugarcane Using Map- and Association-based Approaches

Cited by 31 publications

References 29 publications

Positioning of the major locus for Puccinia psidii rust resistance (Ppr1) on the Eucalyptus reference map and its validation across unrelated pedigrees

Positioning of the major locus for Puccinia psidii rust resistance (Ppr1) on the Eucalyptus reference map and its validation across unrelated pedigrees

QTL mapping including codominant SNP markers with ploidy level information in a sugarcane progeny

Mapeamento genético de cana-de-açúcar (Saccharum spp.) por associação empregando marcadores SSR e AFLP

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