Identification by high-performance liquid chromatography–diode array detection–mass spectrometry and quantification by high-performance liquid chromatography–UV absorbance detection of active constituents of Hypericum perforatum

Brolis, M.; Gabetta, Bruno; Fuzzati, Nicola; Pace, Roberto; Panzeri, F.; Peterlongo, F.

doi:10.1016/s0021-9673(98)00697-9

Cited by 199 publications

(149 citation statements)

References 4 publications

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“…The identification of the constituents was performed using combined HPLC-DAD analysis and HPLC-thermospray MS. performed using rutin as an external standard and consideration of each constituent and the relative response factor (RRF) with respect to rutin, as previously reported [13]. RRF was determined calculating the ratio between the average response factor for each compound and the average response factor of rutin at 270 nm.…”

Section: Hplc-dad Method Calibration and Methods Validationmentioning

confidence: 99%

“…The mobile phase was a five-step (Table 1) linear solvent gradient CH 3 CN/ CH 3 OH/H 2 O (pH 3.2, HCOOH) over a 60-min period at a flow rate of 1 mL/min. The eluent system applied was that reported by Brolis et al [13] which was slightly modified. The injected volume of sample was a 20 lL solution.…”

Section: Generalmentioning

confidence: 99%

“…To evaluate the constituent content, a HPLC analysis was performed using a method described in the literature [13] and modified for our experimental necessities. Quantification of the constituents was performed using rutin as an external standard and consideration of each constituent and the RRF with respect to rutin, as previously reported [13].…”

Section: Quantitative Analysismentioning

confidence: 99%

“…Commercial samples of these naphtho-dianthrones are available; however, the high price is a limiting factor for their application and hinders further pharmacological studies. Another important issue to be considered is the lack of hypericin or pseudohypericin international standards [13] that would permit quantitation of the extracts. Up to now the Brolis et al [13] method using rutin international standard is applied for quantitative analysis of the H. perforatum extracts.…”

Section: Introductionmentioning

confidence: 99%

“…Another important issue to be considered is the lack of hypericin or pseudohypericin international standards [13] that would permit quantitation of the extracts. Up to now the Brolis et al [13] method using rutin international standard is applied for quantitative analysis of the H. perforatum extracts. Therefore, it is essential to find methods of fast and quantitative separation of these substances.…”

Section: Introductionmentioning

confidence: 99%

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Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Karioti¹,

Vincieri

Bilia

2009

J of Separation Science

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Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid-liquid extraction and SEC Hypericin and pseudohypericin, the main naphthodianthrones present in Hypericum species are among the most promising natural products, but the research concerning their biological activities is hindered by their low content in the plant. In this paper a method for the rapid isolation of hypericin and pseudohypericin from Hypericum perforatum hydro-alcoholic dried extracts has been developed. Briefly, the method consists of a partition of the extract between organic and aqueous layers and further purification of the richest extract in naphthodianthrones with Sephadex LH-20 column chromatography. A final separation of hypericin from pseudohypericin was achieved using Sephadex LH-60 column chromatography. All partitions were carried out in triplicate and monitored by LC-MS and NMR analyses. The best results were obtained by successive extraction with n-hexane, Et 2 O and EtOAc. A three-step fractionation resulted in 98% content in total naphthodianthrones. To the best of our knowledge this is the first report on the separation of hypericin from pseudohypericin using size exclusion chromatography.

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Section: Hplc-dad Method Calibration and Methods Validationmentioning

confidence: 99%

Section: Generalmentioning

confidence: 99%

Section: Quantitative Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Karioti¹,

Vincieri

Bilia

2009

J of Separation Science

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High performance liquid chromatography/electrospray mass spectrometry ofHypericum perforatum extracts

Mauri

Pietta

2000

Rapid Commun. Mass Spectrom.

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Hypericum perforatum L. (St. John's Wort) is a widely distributed herbaceous perennial plant which has been well known as a medicinal plant since antiquity. In recent years, H. perforatum has received increasing attention for the treatment of depression and other neuralgic disorders. The main constituents of H. perforatum extract include flavonoids, naphthodianthrones, phloroglucinols, essential oils and xanthones. The present work reports the analysis of naphthodianthrones and phloroglucinols in H. perforatum extracts by means of high performance liquid chromatography (HPLC) coupled simultaneously to a diode array detector (DAD) and electrospray mass spectrometry (ESI-MS). Hypericin, pseudohypericin, hyperforin and adhyperforin were separated and identified on the base of their on-line UV and mass spectra. Quantitative analysis of hypericin derivatives in different extracts of H. perforatum using DAD and MS detectors was performed. In addition, direct infusion ESI-MS of H. perforatum extracts was applied to obtain rapid mass fingerprints of constituents present in the sample.

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Liquid chromatography of natural pigments

Cserháti

Forgács

Morais

et al. 2000

Biomed. Chromatogr.

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The newest results in the application of various liquid chromatographic techniques for the analysis of natural pigments in pure state and in complicated matrices are compiled. The methods employed for the separation and quantitative determination of the different pigment classes (flavonoids, coumarins, chlorophylls, etc.) are described and critically evaluated. The future trends are briefly discussed.

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Identification by high-performance liquid chromatography–diode array detection–mass spectrometry and quantification by high-performance liquid chromatography–UV absorbance detection of active constituents of Hypericum perforatum

Cited by 199 publications

References 4 publications

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

High performance liquid chromatography/electrospray mass spectrometry ofHypericum perforatum extracts

Liquid chromatography of natural pigments

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