Identification, characterization, and expression of two novel P450 genes CYP6BQ21 and CYP6BQ22 from Dastarcus helophoroides (Coleoptera: Bothrideridae)

Li, Feifei; Wang, Haidong; Shaheen, Tayyab; Pei, Pei; Meng-lou, LI

doi:10.1016/j.aspen.2021.07.003

Cited by 1 publication

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…In previous studies, some fragments of CYP6 genes were identified and their expressions in different tissues, developmental stages, during aging and treatment with cypermethrin had been investigated, which indicated their possible roles in the development and pesticide stress of Dastarcus helophoroides. [14][15][16] Nevertheless, the absence of complete genomic information limits the further research on the stress resistance, growth and development, behavior and living habit of Dastarcus helophoroides. Along with the development of new sequencing technologies, the genome assemblies of the relative species and the host Anoplophora glabripennis of Dastarcus helophoroides have been obtained, which provides more information for phylogenetic and genome comparative analysis among them.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chromosome‐level genome assembly of Dastarcus helophoroides provides insights into CYP450 genes expression upon insecticide exposure

Zhang

Pei

Zhang

et al. 2022

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Dastarcus helophoroides is an important natural enemy of cerambycids, and is wildly used in biological control of pests. Nevertheless, the absence of complete genomic information limits the investigation of the underlying molecular mechanisms. Here, a chromosome-level of Dastarcus helophoroides genome is assembled using a combination strategy of Illumina, PacBio, 10x™ Genomics, and Hi-C. RESULTS: The final assembly is 609.09 Mb with contig N50, scaffold N50 and GC content of 5.46 Mb, 42.56 Mb and 31.50%, respectively, and 95.25% of the contigs anchor into 13 chromosomes. In total 14 890 protein-coding genes and 65.37% repeat sequences are predicted in the assembly genome. The phylogenetic analysis of single-copy gene families shared among 20 insect species indicates that Dastarcus helophoroides is placed as the sister species to clade (Nitidulidae+Curculionoidea +Chrysomeloidea) + Tenebrionoidea, and diverges from the related species ∼242.9 Mya. In total 36 expanded gene families are identified in Dastarcus helophoroides genome, and are functionally related to drug metabolism and metabolism of xenobiotics by cytochrome P450. Some members of CYP4 Clade and CYP6 Clade are up-regulated in Dastarcus helophoroides adults upon insecticide exposure, of which expressions of DhCYP4Q, DhCYP6A14X1 and DhCYP4C1 are significantly up-regulated. The silencing of the three genes leads to adults more sensitive to insecticide and increased knocked-down rate, which may indicate their critical roles in stress resistance and detoxication.CONCLUSION: Our study systematically integrated the chromosome-level genome, transcriptome and gene expression of Dastarcus helophoroides, which will provide valuable resources for understanding mechanisms of pesticide metabolism, growth and development, and utilization of the natural enemy in integrated control.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%